33 results about "Acid tolerant" patented technology

Isolated and / or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof are provided. Further provided are methods of at least partially degrading xylotriose, xylobiose, and / or arabinofuranose-substituted xylan using isolated and / or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof.

A dental composition compatible with acidic dental primers / adhesives, the dental composition comprising (A) 5% (w / w) to 90% (w / w) of at least one polymerizable monomer having at least one ethylenically unsaturated vinyl, acrylate, or methacrylate group; (B) 0.01% (w / w) to 10% (w / w) of at least one compound of structurewhere each of R1, R2, and R3 may be the same or different and is independently selected from H, alkyl, alkoxyl, aryl, acyl, allyl, pyridyl, —OH, alkenyl, aralkyl, cycloalkyl, and heterocyclic structure containing O, S, or N; (C) 0.01% (w / w) to 10% (w / w) of at least one hydroperoxide compound with at least one hydroperoxide groups attached to a tertiary carbon; (D) 5% (w / w) to 95% (w / w) of at least one finely divided filler; (E) 0% (w / w) to 25% (w / w) of one or more components selected from a solvent, a photoinitiator, a stabilizer, and / or an antimicrobial agent. One embodiment of the invention is a dental cementation or core buildup kit that has enhanced compatibility between the acidic primer / adhesive and the cement or core buildup material, and therefore enhanced adhesion to a tooth. The kit includes (I) an acidic primer / adhesive composition having a pH less than 4.5; and (II) a cement or core buildup material having the above described composition comprising components (A)-(E). Another embodiment of the invention is a method for adhering a prosthetic device to a tooth to restore the function and / or appearance of a diseased tooth. Another embodiment of the invention is a method of providing a temporary cement, crown and / or bridge, inlay, onlay, endodontic sealer, and / or an endodontic filling material, using the inventive composition.

Disclosed are a novel tactic acid bacterium, Lactobacillus sakei Probio-65, and the use thereof. The L. sakei Probio-65 strain has acid tolerance, bile acid tolerance and antibiotic resistance, inhibits the growth of harmful pathogenic microorganisms in the body and the intestine of animals, and has immunuenhancing activity. In particular, the novel strain inhibits the growth of Staphylocccus aureus, which is known to be a factor aggravating atopic dermatitis. Thus, the novel strain is useful for preventing or treating atopic dermatitis and allergy-related disorders. Also, the novel strain stabilizes intestinal microflors by inhibiting the abnormal proliferation of harmful microorganisms in the intestine. The L. sakei Probio-65 strain or a culture thereof is useful in pharmaceutical, feed, food, and cosmetic compositions.

Disclosed are a novel tactic acid bacterium, Lactobacillus sakei Probio-65, and the use thereof. The L. sakei Probio-65 strain has acid tolerance, bile acid tolerance and antibiotic resistance, inhibits the growth of harmful pathogenic microorganisms in the body and the intestine of animals, and has immunuenhancing activity. In particular, the novel strain inhibits the growth of Staphylocccus aureus, which is known to be a factor aggravating atopic dermatitis. Thus, the novel strain is useful for preventing or treating atopic dermatitis and allergy-related disorders. Also, the novel strain stabilizes intestinal microflors by inhibiting the abnormal proliferation of harmful microorganisms in the intestine. The L. sakei Probio-65 strain or a culture thereof is useful in pharmaceutical, feed, food, and cosmetic compositions.

A novel Lactobacillus plantarum and use thereof are disclosed, which fall within the technical field of microorganisms. The Lactobacillus plantarum tlj-2014 is deposited in China General Microbiological Culture Collection Center (CGMCC) under CGMCC Accession No. 9405. With the strain, the lactic acid production rate can be up to 35 g / L / d, and the concentration of lactic acid after 71 hrs of fermentation is up to 95 g / L. The Lactobacillus plantarum is acid tolerant, survives well at pH 1.80, can degrade the nitrite quickly with a decomposition capability of up to 9.8 mg / h / kg, and is tolerant to 1% bile salt. When the strain is used in production of pickles, the nitrite concentration is less than 5 mg / kg throughout the entire fermentation process, which is far below the content specified in the national standard GB2714-2003.

The present invention relates to a method for the modification of calcium carbonate fillers to improve their acid-tolerant property and to make their effective use in papermaking of high-lignin-content deinked pulp derived from recycled newspaper. It has been found that modification of calcium carbonate fillers using the combination of a calcium salt with a weak acid can improve the acid-tolerant property of the fillers, and the optical properties of the filled paper, such as brightness, can also be effectively improved.

A process for producing lactic acid which includes incubating acid-tolerant homolactic bacteria in nutrient medium to produce a fermentation broth with high levels of free lactic acid is provided. An isolated acid-tolerant homolactic bacteria capable of producing high levels of free lactic acid is also provided.

The invention discloses a heat-resistant and acid-resistant alpha-amylase gene engineering bacterium recombinase and an application method thereof, belonging to the technical field of food biology. The method comprises the following steps: gene engineering recombination, transformation, fermentation process optimization and enzyme activity determination. The heat-resistant and acid-resistant alpha-amylase gene engineering bacterium recombinase disclosed by the invention has the advantages of high enzyme activity, high tolerable temperature, wide tolerable pH value and convenience in use.

A multi-layer membrane structure including a crosslinked polymeric membrane, such as a crosslinked polyvinyl sulfate membrane or a crosslinked copolymer polyvinyl sulfate and polyvinyl alcohol membrane, is provided. The membrane structure is suitable for use in an acid environment, and is suitable for recovering acid from a feed mixture comprising acid, hydrocarbons and water.

The invention relates to a strain of streptomyces albidus AAE89, which is named as Streptomyces albidus AAE89 in Latin. The strain is preserved in China General Microbiological Culture Collection Center on October 12, 2020, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC 20880; the streptomyces albidosus AAE89 provided by the invention has stronger acid resistance, and compared with an original strain, the streptomyces albidosus AAE89 has the advantages that the dry weight of a thallus is increased by 46.92% after the streptomyces albidosus AAE89 is cultured for 48 hours under the condition that the initial pH is 4.0, and the survival rate of the thallus is increased by 1.81 times after the thallus is stressed for 48 hours under the condition that the pH is 3.0.

A novel Lactobacillus plantarum and use thereof are disclosed, which fall within the technical field of microorganisms. The Lactobacillus plantarum tlj-2014 is deposited in China General Microbiological Culture Collection Center (CGMCC) under CGMCC Accession No. 9405. With the strain, the lactic acid production rate can be up to 35 g / L / d, and the concentration of lactic acid after 71 hrs of fermentation is up to 95 g / L. The Lactobacillus plantarum is acid tolerant, survives well at pH 1.80, can degrade the nitrite quickly with a decomposition capability of up to 9.8 mg / h / kg, and is tolerant to 1% bile salt. When the strain is used in production of pickles, the nitrite concentration is less than 5 mg / kg throughout the entire fermentation process, which is far below the content specified in the national standard GB2714-2003.

A crosslinked polymeric membrane, such as a crosslinked polyvinyl sulfate membrane or a crosslinked copolymer polyvinyl sulfate and polyvinyl alcohol membrane, is provided. The membrane is suitable for use in an acid environment, and is suitable for recovering acid from a feed mixture comprising acid, hydrocarbons and water.

The invention discloses an oxygen-resistant, acid-resistant, and high-sugar-resistant Propionibacterium acidogen, which is classified as Propionibacterium acidigens ( Propionibacterium acidipropionici ) L1124, deposit number CGMCC No.14869. The bacterial strain of the invention has strong tolerance to oxygen, acid and osmotic pressure; and can overcome the problems of long growth cycle and low yield of anaerobic bacteria in the fermentation process.

The invention belongs to the field of biotechnology, and in particular relates to a sugar-resistant and acid-resistant β-glucosidase and its production. The β-glucosidase is produced from Aspergillus niger AN-4, and the preservation number is CGMCC No.14139. The average shake flask fermentation activity of β-glucosidase resistant to high sugar concentration produced by Aspergillus niger AN-4 is above 523U / mL; It is relatively stable within the range; thermal stability is good, the optimum reaction temperature range is 45-65°C, and it still has 75% activity at 75°C for 1 hour; and it has good glucose tolerance. It has good effect in cellulose degradation, flavoring agent and other applications, and can be widely used in food, medicine, chemical industry and other industries.

The invention relates to the field of bioengineering, particularly discloses a signal recognition particle subunit shown in SEQ ID No:1 and / or application of a nucleotide sequence encoding the signal recognition particle subunit to preparation of acid-resistant bifidobacterium, and further discloses a recombinant expression plasmid containing the nucleotide sequence and the acid-resistant bifidobacterium containing the signal recognition particle subunit and / or the nucleotide sequence encoding the signal recognition particle subunit and / or the acid-resistant bifidobacterium of the recombinant expression plasmid. According to the technical scheme, a gene encoding the signal recognition particle subunit shown in SEQ ID No:1, preferentially a plasmid P18P containing the gene is converted into bifidobacterium which is preferred as bifidobacterium CGMCCNo.2265, and the gene can resistant to acid with the pH being 1, the preferential pH being 2 and the optimal pH being 3.5.

A super-resolution imaging dye for acid-fast bacteria and its synthesis method and application. The specific molecular structure of the dye is rhodamine spiramide substituted with succinimide active ester as the basic structural unit, and its structural formula is shown in (1): The super-resolution imaging dye for acid-fast bacteria not only has acid-resistant performance, but also retains the visible light activation performance. Therefore, this kind of acid-fast bacteria super-resolution imaging dye can be applied in super-resolution imaging technology without interference from acidic environment.

The invention discloses a method for improving the acid stress resistance of T. glabrata, belonging to the field of biological engineering. The present invention constructs a gene deletion mutant strain Cgade12Δ by transforming T. glabrata, measures the intracellular ATP level of the constructed strain, and analyzes the organic acid tolerance of the strain through a plate growth experiment and cell activity. After the deletion of the gene CgADE12 was found, under 0.2% acetic acid stress, the intracellular ATP level of the strain decreased, the growth ability under acetic acid stress increased, and the acetic acid tolerance was enhanced.

The invention discloses an acid-resistant flocculation industrial Saccharomyces cerevisiae strain and its construction method, which solves the problem that most of the strains fused by the existing fusion technology can only effectively have the genetic traits of both parents, but cannot effectively improve the genetic traits of the fused strains. . The invention includes that the strain of Saccharomyces cerevisiae SEB4 is preserved in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC11324. The invention also provides the construction method of the Saccharomyces cerevisiae SEB4. In the present invention, the fused Saccharomyces cerevisiae SEB4 strain not only has the genetic traits of both parents, but also has the good flocculation and fermentation ability of the parent fusion son No. 1, and the good acid resistance of the parent SEB2; and its ethanol production and glucose consumption rate. were superior to the parental strains.