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An acid-resistant flocculation industrial Saccharomyces cerevisiae strain and its construction method

A Saccharomyces cerevisiae strain and technology of Saccharomyces cerevisiae, which are applied in the field of acid-resistant flocculating industrial Saccharomyces cerevisiae strains, can solve the problems such as the inability to effectively improve the genetic properties and performance of the strains after fusion, and achieve the effect of improving performance

Active Publication Date: 2019-01-04
SICHUAN UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that most of the bacterial strains fused by the existing fusion technology can only effectively have the genetic traits of both parents, but cannot effectively improve the performance of the genetic traits of the strain after fusion; provide a strain that can effectively have the genetic traits of both parents, and simultaneously An acid-resistant flocculation-resistant industrial Saccharomyces cerevisiae strain that is superior to the parent strain in ethanol production, glucose consumption and consumption rate, and provides a construction method for the strain

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  • An acid-resistant flocculation industrial Saccharomyces cerevisiae strain and its construction method
  • An acid-resistant flocculation industrial Saccharomyces cerevisiae strain and its construction method
  • An acid-resistant flocculation industrial Saccharomyces cerevisiae strain and its construction method

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Embodiment 1

[0038] An acid-resistant flocculation industrial Saccharomyces cerevisiae strain, said Saccharomyces cerevisiae ( Saccharomyces cerevisiae The SEB4) strain is preserved in the China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC11324. The specific construction method of the Saccharomyces cerevisiae strain SEB4 is as follows:

[0039] 1) Construction of auxotrophic mutant bacteria

[0040] 1.1) Take Saccharomyces cerevisiae Saccharomyces cerevisiae IR-2 and Saccharomyces cerevisiae Saccharomyces cerevisiae SEB1 was the parent, and the fusion son RHZ-1 was obtained through cell fusion.

[0041] The SEB1 strain in this example has the ability to produce ethanol and is preserved in the China General Microorganism Culture Collection and Management Center with the preservation number CGMCC11321. IR-2 strain: isolated from a fermented food in Indonesia, with ethanol production ability and flocculation, the source is recor...

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Abstract

The invention discloses an acid-resistant flocculation industrial Saccharomyces cerevisiae strain and its construction method, which solves the problem that most of the strains fused by the existing fusion technology can only effectively have the genetic traits of both parents, but cannot effectively improve the genetic traits of the fused strains. . The invention includes that the strain of Saccharomyces cerevisiae SEB4 is preserved in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC11324. The invention also provides the construction method of the Saccharomyces cerevisiae SEB4. In the present invention, the fused Saccharomyces cerevisiae SEB4 strain not only has the genetic traits of both parents, but also has the good flocculation and fermentation ability of the parent fusion son No. 1, and the good acid resistance of the parent SEB2; and its ethanol production and glucose consumption rate. were superior to the parental strains.

Description

technical field [0001] The invention relates to a yeast strain, in particular to an acid-resistant flocculation industrial Saccharomyces cerevisiae strain and a construction method of the strain. Background technique [0002] Biofuel ethanol is considered to be an important energy source that can replace traditional fuel oil. Saccharomyces cerevisiae is the main microorganism used in the industrial production of bioethanol, and the optimum pH value of general strains is 4-5. When the pH was higher or lower than this range, the growth and fermentative ability of the strain decreased. However, large-scale industrial production requires good control of bacterial contamination, and it is an effective measure to reduce the pH value of the fermentation system as much as possible. In addition, lignocellulosic biomass is an important raw material for the production of fuel ethanol, and the first step in its utilization requires pretreatment with dilute acid. For ethanol fermentat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12N15/04C12N13/00C12R1/865
Inventor 汤岳琴木田建次苟敏孙照勇
Owner SICHUAN UNIV
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