32results about How to "Improve acid stress resistance" patented technology

The invention discloses a method for using the transcription factor Crz1p to regulate acid stress resistance of Toruula glabrata, belonging to the field of bioengineering. In the present invention, by deleting or overexpressing the Cgcrz1 gene of Torulopsis glabrata, the acid stress resistance of the strain is correspondingly reduced or improved. The present invention also compares the cell membrane fatty acid, sterol composition, ratio and permeability of the deletion mutant strain Cgcrz1Δ under acid stress. Acid stress resistance.

The invention discloses a glyceraldehyde-3-phosphate dehydrogenase and applications thereof, and belongs to the technical field of genetic engineering and microbial engineering. The glyceraldehydes-3-phosphate dehydrogenase with an amino acid sequence as shown in SEQ ID No.1 has the function of helping microorganisms produce triacylglycerol (TAG). By culturing the recombinant Mortierella alpina containing the glyceraldehydes-3-phosphate dehydrogenase in a shaking table for 7 days, the total fatty acid content and the polyunsaturated fatty acid content of the Mortierella alpina containing the glyceraldehydes-3-phosphate dehydrogenase can be increased by 12.56% and 12.79% respectively, compared with the Mortierella alpina containing no glyceraldehydes-3-phosphate dehydrogenase. The result provides abundant theoretical support for further improving the capability of oil-producing microorganisms such as Mortierella alpina to produce triacylglycerol (TAG) and further improving the capability of microorganisms to produce polyunsaturated fatty acid (PUFAs) through a genetic engineering means.

The invention discloses a lactic acid bacteria engineering bacterium with improved acid stress resistance and an application thereof, belonging to the technical fields of genetic engineering and microbial engineering. The present invention takes the gene encoding metal ABC transporter permease ZitP (metal ABC transporter permease) as the target gene, and uses lactic acid bacteria as the expression host to successfully construct a kind of protein that can be widely used in the preparation of food, medicine, feed and chemicals. Lactic acid bacteria engineering bacteria: The acid stress resistance of this lactic acid bacteria engineering bacteria has been significantly improved, up to 14.5 times higher than that of wild strains.

The invention discloses an acid stress resistant component and application thereof, and belongs to the field of bioengineering technology. By overexpression of CmhR gene derived from L. lactis NZ9000in L. lactis NZ9000, recombinant L. lactis NZ9000 (pNZ8148 / CmhR) with acid stress resistance remarkably enhanced is obtained. By stress for 3 h at pH 4.0, survival rate of the recombinant strain is 12.3 times higher than survival rate of a contrast. The invention also provides a method for raising acid stress resistance. The method of the invention has good industrial application value.