The invention discloses a technology for producing
glutathione by fermenting
saccharomyces cerevisiae and belongs to the field of industrial microbial
fermentation. The technology for producing
glutathione by fermenting
saccharomyces cerevisiae comprises the following steps: utilizing an improved O-media
fermentation medium to ferment for 16h, and then adding molasses and corn liquor at
constant speed of 1.5g / L / h and 0.4g / L / h; after the
cell density reaches a certain degree, adding a certain amount of KMnO4 slow-released grains and
sodium citrate into the
fermentation liquor; fermenting for 120h so that the
cell wet weight in the fermentation liquor reaches up to 121.4g / L and the GSH accumulated volume reaches up to 5.78g / L. According to the invention, the
cell density of the fermentation liquor is promoted through the improvement for the O-media fermentation medium and the flow adding of the molasses and corn liquor; the
oxidative stress for
yeast cells is maintained and meanwhile the influence thereof on the
yeast growth is relieved through the addition of oxidized
stimulant KMnO4 slow-released grains;
intracellular ATP level is improved through the addition of the energy auxiliary matter
sodium citrate, and finally, the accumulated volume of
glutathione is obviously improved and the production cost is lowered.