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Use of composition in prevention or treatment of neurodegenerative diseases

A neurodegenerative and composition technology, applied in the field of prevention or treatment of neurodegenerative diseases, composition, can solve problems such as incurable, highly disabling, and incompletely clear pathogenesis

Active Publication Date: 2020-07-31
BGI INST OF APPLIED AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For many years, PD has been a hot research area in the medical field because of its high prevalence, high disability and inability to cure, but the pathogenesis of PD has not yet been fully clarified.

Method used

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  • Use of composition in prevention or treatment of neurodegenerative diseases
  • Use of composition in prevention or treatment of neurodegenerative diseases
  • Use of composition in prevention or treatment of neurodegenerative diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Cell culture and PD cell model establishment

[0101] Experimental materials: Human neuroblastoma cells (SH-SY5Y) were purchased from the Kunming Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences.

[0102] Cell culture:

[0103] The medium was RPMI-1640 medium containing 10% fetal bovine serum, and the culture condition was 5% CO 2 , 37 ℃ constant temperature.

[0104] Modeling and administration methods:

[0105] SH-SY5Y cells were seeded in 96-well plates (1×10 4 per hole), placed in 5% CO 2 , and incubated in a 37°C incubator. After 4 hours of cell attachment, the medium was discarded and replaced with a serum-free medium containing (or not) 100 μmol / L lipoic acid (LA) or 20-50% w / w sacha inchi oil (SIO) in the incubator After incubation for 1 h, add with (or without) 2mmol / L MPP + Incubate in serum-free medium for 24 h.

[0106] MTT assay for cell viability:

[0107] SH-SY5Y cells were seeded in 96-well plates (1×10 4 pe...

Embodiment 2

[0119] Effects of different concentrations of lipoic acid and / or Sacha Inchi oil on cell viability:

[0120] SH-SY5Y cells were seeded in 96-well plates (1×10 4 per hole), placed in 5% CO 2 , and incubated in a 37°C incubator. After 4 hours of cell attachment, the medium was discarded, and (1) containing MPP at a concentration of 0, 1, 2, 5mmol / L was added + or (2) serum-free medium containing 0, 12.5, 25, 50, 100 μmol / L LA or 20-50% w / w SIO, incubated in an incubator 24h; or (3) serum-free medium containing (or not containing) 0, 12.5, 25, 50, 100 μmol / L LA or 20-50% w / w SIO, after incubation in the incubator for 1 hour, add containing (or not Including) 2mmol / L MPP + Incubate in serum-free medium for 24 h. Discard the old medium, add 100 μl of MTT solution containing 0.5 mg / mL to each well, and incubate in the incubator for more than 4 hours. Remove the culture medium, add 100 μl of DMSO to each well to dissolve the blue-purple crystals, place the 96-well plate at 37°C...

Embodiment 3

[0124] Effects of different concentrations of lipoic acid and / or Sacha Inchi oil on mitochondrial membrane potential (Δψ):

[0125] SH-SY5Y cells were seeded in 96-well plates (1×10 4 per hole), placed in 5% CO 2 , and incubated in a 37°C incubator. After 4 hours of cell attachment, the medium was discarded and replaced with a serum-free medium containing (or not containing) 100 μmol / L LA and 20% or 50% w / w SIO. After incubation in the incubator for 1 hour, adding containing (or Excluding) 2mmol / L MPP + Incubate in serum-free medium for 24 h. After the incubation, add JC-1 fluorescent staining solution with a concentration of 10 μg / mL, and incubate in the incubator for 1 h. Finally, the fluorescence value was detected in a full-wavelength multifunctional microplate reader (green light excitation wavelength 485nm, emission wavelength 538nm; red light excitation wavelength 485nm, emission wavelength 585nm), and the final mitochondrial membrane potential result was presented ...

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Abstract

The invention provides a pharmaceutical composition and application of the composition in prevention or treatment of neurodegenerative diseases. The pharmaceutical composition comprises sacha inchi oil and / or lipoic acid, and is used for at least one of the following functions: preventing and / or treating neurodegenerative diseases, increasing the survival rate of nerve cells, reducing apoptosis ofnerve cells, increasing the mitochondrial membrane potential of nerve cells, protecting mitochondrial functions and increasing the ATP level in nerve cells. The pharmaceutical composition is good ineffect of preventing and / or treating neurodegenerative diseases, is small in side effect and is capable of effectively increasing the survival rate of nerve cells, effectively protecting mitochondrialmembrane potential of the nerve cells and effectively protecting the energy metabolism level of the nerve cells.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular, the invention relates to the use of the composition in preventing or treating neurodegenerative diseases. Background technique [0002] In aging and neurodegenerative diseases, inflammation, oxidative stress, and decreased mitochondrial function are common features of such neurological diseases, such as Alzheimer's disease, Parkinson's disease, Huntington's disease, etc. Oxidative damage can lead to energy conversion system disorders in mitochondria, which in turn damages mitochondrial functional activity. [0003] The pathogenesis of neurodegenerative diseases often involves mitochondrial dysfunction, which is further exacerbated by the downregulation of cytoplasmic glutaredoxin-1, which is responsible for regulating the redox state of voltage dependent anion channel (VDAC) and maintaining mitochondrial integrity. Phenomenon. [0004] Parkinson's Disease (PD) is a common neurodegenera...

Claims

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Application Information

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IPC IPC(8): A61K36/47A61P25/28A61P25/16A61K31/385
CPCA61K36/47A61K31/385A61P25/28A61P25/16A61K2300/00
Inventor 宋歌邢杰代晓霜冷冰峰李雪静
Owner BGI INST OF APPLIED AGRI
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