57results about How to "Achieve high density culture" patented technology

The present invention discloses a high density culture method and applications of probiotics. According to the present invention, a porous material is added to a culture medium containing a growth promoting factor for promoting the growth and the breeding of probiotics, a volume ratio of the culture medium to the porous material is 1:0.001-1:1000, and probiotics are inoculated in the culture medium and culturing is performed so as to achieve the high density culture of the probiotics; the obtained probiotics can be used for preparing food, food additives, medicines, feeds or environmental protection treatment, and other products; and the obtained high-density probiotics have advantages of good heat resistance, good acid resistance , good drug resistance and other performances, and have wide application prospects in food, medicines, feeds, environmental protection, and other fields.

The invention relates to a method for producing polypeptide antibiotics enramycin by the fermentation method, which pertains to the fields of agricultural biotechnology, industrial biotechnology and the fermentation engineering. The method is as follows: (1) slant culture: a Streptomyces sp.NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days; (2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days; (3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

The invention discloses bacillus subtilis cultured by biochar and a preparation method and an application thereof. The preparation method takes biochar as an adsorption carrier, such as wood chips, animal bones, plant rhizomes and other biochar, takes any kind of bacillus subtilis strains as a starting strain, and successively comprises the following steps: (1) test tube expanding culture; (2) liquid shake flask culture; (3) seed tank expanding culture; (4) fermentation culture; and (5) drying. The bacterial density of the bacillus subtilis prepared by the method can reach 5*10<13>-1*10<15> cfu / mL. The biological adsorption and the liquid fermentation are in organic combination, the bacterial density of the liquid fermentation is improved, no any additional equipment is added, continuous culture and semi continuous culture are not used, and the high-density culture of the bacillus subtilis is achieved; and the cultured bacillus subtilis can be used as a live bacterial agent of a biofertilizer or used as a live bacterial agent of the biofertilizer with other strains.

The invention discloses a production process for fermenting trichoderma spp by taking orange peels as raw materials, which belongs to the technical field of microorganisms. The production process comprises the following steps: (1) preprocessing: drying, grinding and sieving the orange peels; (2) mixing preprocessed orange peel powder with wheat bran and de-ionized water according to a certain ratio, and sterilizing by high-pressure steam; (3) inoculating trichoderma onto a sterilized material for solid-state fermentation; (4) drying a solid fermentation product to obtain a trichoderma preparation product. The trichoderma spp preparation produced by the process has capabilities of promoting plant growth and inducing plants to resist adversity and diseases, can be used for preventing and treating soil-borne diseases and promoting repair of plants in polluted soil, and provides a new way for resource utilization of the orange peels.

The invention discloses an efficient culture medium for chlorella. The efficient culture medium for the chlorella comprises the following components per liter: (1) major elements: 0.10-0.20g of sodiumnitrate, 0.01-0.04g of potassium dihydrogen phosphate, 0.02-0.08g of magnesium sulfate heptahydrate, 0.02-0.06g of urea, 0.01-0.04g of calcium chloride, 0.01-0.05g of sodium bicarbonate and 0.50-1.5gof sodium chloride; (2) microelements: 2.00-5.00mg of ferric citrate, 0.05-0.10mg of copper sulfate pentahydrate, 2.00-3.00mg of boric acid, 1.50-2.00 mg of tetrahydrate manganese chloride and 0.10-0.40 mg of sodium molybdate dihydrate; and the balance of seawater. The efficient culture medium for the chlorella can be directly added and applied, so that the chlorella can quickly absorb various nutrients in a liquid medium in a short time, thereby realizing high-density culture, and achieving faster growth rate and higher yield; at the same time, the cost of buying agar powder is saved, thereby reducing the production cost.

The invention provides a production method of an encephalitis B vaccine, which comprises the following steps: adding Vero seed cells to a 300-liter bioreactor, and carrying out perfusion culture together with a microcarrier; after the perfusion culture is carried out for 5-6 days, depositing the microcarrier and the cells, replacing a culture medium with a main medium, vaccinating encephalitis B viruses, and continuously carrying out the perfusion culture; and collecting a virus culture solution, filtering, inactivating and purifying the virus culture solution. By using the method, the density of the cells for production is improved; the production capacity is improved greatly; the large-scale and high-density culture is achieved; the difference between batches of products and the instability of a culture period are eliminated; the quality of the products is guaranteed to be stable and uniform; the product quality is improved; the difficult problems of the amplification culture process of the bioreactor are solved; the high-density and large-scale culture of mammalian cells by using the bioreactor is achieved.

The invention discloses a method for large-scale high-density suspension culture of a cell 293-high yielding adenovirus. The method comprises the steps of firstly, carrying out serum-free 293SFM culture, then, starting CD culture medium culture to produce a virus, combining a perfusion technology and a cutoff technology, and meanwhile, controlling temperature changes of cell culture and viral infection. According to the method, the enlarging of cell culture density is achieved, the volume of cell culture is reduced, subsequent labor intensity is lowered, the yield of the virus is increased, the cost is reduced, the cell death loss of a cutoff process is reduced, the quality is uniform and stable during culture, a production process is close in link and easy in control, and thus, stable quality of batches is guaranteed.

The invention discloses an animal cell hollow fiber culture system and a cell culture method thereof. The system comprises a hollow fiber cylinder, a liquid storage system, circulating systems (an inner circulating system and an outer circulating system) and a detection control system. The hollow fiber cylinder performs culture medium circulation through the inner circulating system; and the outercirculating system is used for cell inoculation and cell digestion, uniform distribution of cells among hollow fibers is achieved through circulation of a peristaltic pump in the outer circulating system, and the cell inoculation uniformity is improved. Conditions similar to in-vivo growth are established, and the culture effect is good. An alternate positive pressure circulating system (mode) isadopted to guarantee alternate replacement of nutrient components in hollow fiber inner and outer systems, and the problems that hollow fibers are blocked and the dead volume of a nutrient solution cannot be replaced are also avoided. Meanwhile, the system is of a closed design, is good in biological safety, can effectively control pH, dissolved oxygen and temperature in the culture process, andis suitable for animal cell culture in vaccine and antibody production.

The invention provides a lactic acid bacteria fermentation medium for feed and a preparation method thereof, belonging to the technical field of microorganisms, wherein the fermentation medium comprises whey protein hydrolysate obtained by acid hydrolysis of whey protein with inulin. The preparation method of fermentation medium comprises: preparing whey protein hydrolysate: carrying out acid hydrolysis on whey protein added with inulin; and mixing the resulting whey protein hydrolysate, yeast powder, glucose, buffer salt, Mg<2+> water-soluble compound, Fe<2+> water-soluble compound, and MnSO4. As that fermentation medium of the invention can prevent the damage of oxygen to the feed lactic acid bacteria, more ATP is provided for the feeding lactic acid bacteria, the growth requirement ofthe fermentation medium for the feed lactic acid bacteria is prolonged, the acid stress resistance and the propagation quantity of the feeding lactic acid bacteria are improved, and high-density culture is realized. The whey protein hydrolysate prepared by the preparation method of the invention comprises D Type amino acids and flavoring amino acids.

The invention discloses a method of yeast fermentation coproduction ergot sterol and glutathion, utilizing conspecific yeast bacterial fermentation to produce simultaneous two kinds of metabolite-ergot sterol and glutathion, the utilization bacterial of fermentation is Saccharomyces cerevisiae, candida utilis, candida tropicalis or engineering bacterial reconstructed by mutagenesis and gene engineering, the utilization yeast culture medium contain the carbon source such as glucose, cereal mash, sucrose molasses and so on, the nitrogen source such as maize milk, yeast powder, protease leather, carbonyldiamide, ammonia and so on, inorganic salt and the metallic ion that the yeast need. The invention utilizes fermentation coproduction ergot sterol and glutathion, simultaneously, accomplish high-density culture of the yeast cell, and make the biomass of yeast expression coproduction come up to the standard of the high level in individual fermentation ergot sterol and glutathion, full utilization culture medium substrate, increasing availability ratio of raw material, decreasing the cost of manufacture.

The invention relates to a method for producing polypeptide antibiotics enramycin by the fermentation method, which pertains to the fields of agricultural biotechnology, industrial biotechnology and the fermentation engineering. The method is as follows: (1) slant culture: a Streptomyces sp.NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days; (2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days; (3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

The invention relates to a high-density and large-scale diatom carrier culture technique which comprises the steps that many kinds of seaweed meal serve as carriers; 0.001-50% of seaweed meal is added to seawater; an appropriate amount of nutritive salt is added; a diatom seed solution is inoculated; natural or artificial light is adopted; the diatom is cultured at a natural temperature of 30 DEGC for 2-30 days; and ventilation or agitation is performed appropriately in a culture process. Diatom is attached to the surface of seaweed, grows and propagates. After the culture, a seaweed and diatom mixture is separated and obtained by separation methods such as natural settling, filtering and centrifugation, contains more than thirty million diatom cells per gram in wet weight, and can serve as fresh and alive fish bait to be fed directly. The separated seawater can be used for the culture next time directly after the nutritive salt, the seaweed meal and the diatom seed solution are added. The culture technique is convenient and easy to implement, and is applicable to reactor culture and large-scale pond culture of benthic diatom; high-density fresh and alive diatom biomass can be obtained; and the alive diatom cells serve as feed to be fed, and can exert a function of improving a substrate environment.

The invention provides a positive displacement conveying tube type photobioreactor which is composed of a positive displacement transmission system, a photosynthetic reaction system and supporting accessories.The positive displacement transmission system is composed of a gear, a transmission shaft, a motor, a sealing plug, a chain, an inlet cone and an upper water tube, wherein the gear is driven to rotate by the motor, an alga solution in the upper water tube is conveyed into the photosynthetic reaction system in a sectioned and sealed mode through the sealing plug via chain transmission, and a power source is provided for circulation of the alga solution.The photosynthetic reaction system comprises photosynthetic reaction tube sections, a water distribution trough, a water trough, a connecting tube and a water outlet tube.Unit combination rotors are installed in the photosynthetic reaction tube sections, and a reaction site is provided for a photosynthetic reaction of microalgae.The supporting accessories include a motor rack, a gear supporting plate and a supporting frame, and support and fix the whole system.According to the positive displacement conveying tube type photobioreactor, microalgae cells are protected against harm of pump shearing force, and high-density culture of microalgae can be easily achieved; the number of the photosynthetic reaction tube sections connected to the water distribution trough can be adjusted according to flow, the flow speed and the culture scale, the structure is flexible, and adaptability is high.

The invention discloses a pipeline type photobioreactor for culturing microalgae in multiple nutrition modes. The pipeline type photobioreactor comprises a plurality of groups of reaction units and acirculating unit, wherein each reaction unit comprises front and rear rows of transparent pipelines; the circulating unit comprises a buffer tank and a circulating pump, wherein the buffer tank comprises a tank body, a cover body and a heating jacket, a heating device is also arranged in the heating jacket, and a pH sensor and a temperature sensor are arranged in the tank body; a discharge headerpipe is arranged at the bottom of the tank body, the discharge header pipe communicates with an inlet of the circulating pump, an outlet pipeline of the circulating pump is divided into feeding branches, and the feeding branches communicate with feeding ports of the reaction units; a material collecting pipeline is arranged on each feeding branch; a discharge hole of each reaction unit communicates with the circulating main pipe through a discharge pipeline; the circulating header pipe communicates with the buffer tank; a discharge branch pipe is also arranged on the discharge header pipe; a breather valve, an inoculation port, a carbon dioxide pipeline, a nutritive salt supplementing pipeline and a turbidity testing pipeline are arranged on the cover body; and the turbidity testing pipeline communicates with a turbidity detection device.