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Method for simultaneous production of ergosterol and glutathione by yeast fermentation

A technology for ergosterol and yeast fermentation, applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of low utilization rate of raw materials and high cost, achieve improved utilization rate, reduced production cost, and promoted product synthetic effect

Active Publication Date: 2006-10-11
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In summary, what the existing technology does is to use yeast culture to produce ergosterol or glutathione alone, which has problems such as low raw material utilization and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: Cultivate Candida tropicalis to produce ergosterol and glutathione

[0015] Use a 5L general-purpose fermenter to prepare a 3L fermentation medium. The medium formula: glucose 40g / L, corn steep liquor 45g / L, wort juice 20g / L, magnesium sulfate 4.5g / L, dipotassium hydrogen phosphate 12g / L, diphosphate Potassium hydrogen 12g / L, Zn 2+ 14ppm, Fe 2+ 、Cu 2+ , Mn 2+ 6ppm each, sterilized in real tank at 121°C for 30 minutes. After the medium temperature is down to 30°C, inoculate by 10% (volume ratio of seed liquid and fermented liquid) inoculum, adjust the ventilation ratio to be 1.3VVM, and the stirring speed is 400rpm, start fermentation, ferment for 12 hours, the fermented liquid The temperature control range is 20-35°C. At this time, the ethanol concentration in the fermentation broth is reduced to 50g / L, and the concentration of glucose, molasses and corn steep liquor is added at a concentration of 700g / L, and the flow acceleration rate is adjusted to m...

Embodiment 2

[0016] Embodiment 2: Cultivate Candida utilis to produce ergosterol and glutathione

[0017] Use a 5L general-purpose fermenter for fermentation. Prepare 3L fermentation medium, medium formula: glucose 60g / L, corn steep liquor 30g / L, wort juice 10g / L, diammonium hydrogen phosphate 7g / L, magnesium sulfate 6g / L, dipotassium hydrogen phosphate 10g / L, phosphoric acid Potassium dihydrogen 20g / L, Zn 2+ 15ppm, Fe 2+ 、Cu 2+ , Mn 2+ 2ppm each, sterilized in real tank at 121°C for 30 minutes. After the medium temperature drops to 30°C, inoculate with an inoculation amount of 10% (the volume ratio of seed liquid to fermentation liquid), adjust the ventilation ratio to 1.0VVM, and the stirring speed to 300rpm to start fermentation. The temperature control range of the fermented liquid is 20-35 DEG C, ferment for 13 hours, at this moment, the concentration of ethanol in the fermented liquid is down to 45g / L, start feeding glucose, corn steep liquor and molasses aqueous solution with a...

Embodiment 3

[0018] Example 3: Cultivating Saccharomyces cerevisiae to produce ergosterol and glutathione

[0019] Use a 5L general-purpose fermenter for fermentation. Prepare 2.5L fermentation medium, medium formula: glucose 60g / L, corn steep liquor 44g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 3g / L, Zn 2+ 12ppm, Fe 2+ 、Cu 2+ , Mn 2+4ppm each), and sterilized in real tank at 121°C for 30 minutes. After the medium temperature drops to 30°C, inoculate with an inoculation amount of 10% (volume ratio of seed liquid to fermentation liquid), adjust the ventilation ratio to 1.0VVM, and the stirring speed to 200rpm to start fermentation. The temperature control range of the fermentation broth is 20-35°C. From the 10th hour after fermentation, the glucose solution with a concentration of 600g / L and the corn steep liquor solution with a concentration of 100g / L are added. The fermentation proceeds to 22 hours, and the ethanol concentration drops to 17g / L, at this time, the flow ...

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PUM

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Abstract

The invention discloses a method of yeast fermentation coproduction ergot sterol and glutathion, utilizing conspecific yeast bacterial fermentation to produce simultaneous two kinds of metabolite-ergot sterol and glutathion, the utilization bacterial of fermentation is Saccharomyces cerevisiae, candida utilis, candida tropicalis or engineering bacterial reconstructed by mutagenesis and gene engineering, the utilization yeast culture medium contain the carbon source such as glucose, cereal mash, sucrose molasses and so on, the nitrogen source such as maize milk, yeast powder, protease leather, carbonyldiamide, ammonia and so on, inorganic salt and the metallic ion that the yeast need. The invention utilizes fermentation coproduction ergot sterol and glutathion, simultaneously, accomplish high-density culture of the yeast cell, and make the biomass of yeast expression coproduction come up to the standard of the high level in individual fermentation ergot sterol and glutathion, full utilization culture medium substrate, increasing availability ratio of raw material, decreasing the cost of manufacture.

Description

technical field [0001] The invention relates to a high-density culture of yeast cells and a method for producing ergosterol and glutathione through the yeast cells, belonging to the field of biological fermentation engineering. Background technique [0002] Both ergosterol and glutathione are important medical and health products. At present, it is the most common method to produce ergosterol or glutathione by fermentation at home and abroad, that is, to produce ergosterol or glutathione by cultivating Yeast gets the product. In order to improve the level of products produced by this method, people have selected (or constructed) strains with strong synthetic ability and high intracellular content, screened and optimized the medium formula, established and optimized fermentation control, improved and improved downstream engineering technology, etc. To improve productivity and quality. For example, Wenck used Aspergillus to produce ergosterol, and the ergosterol content acco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12P21/02C12R1/85
Inventor 谭天伟王玺尚飞
Owner BEIJING UNIV OF CHEM TECH
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