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Rapid culture method of chrysophyceae

A culture method and technology of golden algae, applied in the field of rapid cultivation of golden algae, can solve the problems of low culture density, slow growth rate of culture, difficulty in cultivating golden algae biomass to meet the requirements of industrial processing and application, and achieve energy saving, The effect of stable efficiency

Pending Publication Date: 2019-05-28
德默特生物科技(珠海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth rate of photosynthetic culture of golden algae is very slow, and the culture density is also very low, so it is difficult to quickly obtain golden algae biomass that can be processed and utilized industrially
According to the current culture technology, the culture speed of golden algae biomass is difficult to meet the requirements of industrial processing and application

Method used

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  • Rapid culture method of chrysophyceae
  • Rapid culture method of chrysophyceae
  • Rapid culture method of chrysophyceae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Chrysophytes were cultured in a 7.5 L, New Brunswick (NBS) BioFlo310 fermenter.

[0058] Cultivated in a 7.5L fermenter with this inventive method, the highest cell number can reach 2.88×10 8 Each mL, the cell biomass can reach 56g / L.

[0059] Step 1: Photoautotrophic pre-cultivation of seeds

[0060] (1) Prepare the seed culture medium according to Table 1, divide it into 1L Erlenmeyer flasks with a volume of 300mL, and sterilize at a high temperature of 121°C for 30 minutes.

[0061] (2) Inoculation, algae species Poteriochromonas malhamensis CMBB-01 was provided by the Institute of Hydrology, Chinese Academy of Sciences, Wuhan, with an inoculation volume of 10 mL.

[0062] (3) Culture for 7 days, culture temperature 25-28 ℃, light intensity 30-50 μmol photons m -2 the s -1 . When the seed color changes from golden yellow to dark brown, the dry weight can reach 2-3g / L.

[0063] Step 2: Fermentation tank culture

[0064] (1) Prepare fermenter culture medium a...

Embodiment 2

[0079] Chrysophytes were cultured in a 125 L, New Brunswick (NBS) BioFlo610 fermenter.

[0080] The enlarged culture is carried out on a 125L fermenter with the method of the invention, and the biomass of the golden algae cells can reach 45 g / L after 96 hours of culture.

[0081] Step 1: Seed Preparation

[0082] After cultivating 96h among the embodiment 1, by figure 1It can be seen that it is in the logarithmic phase and is suitable for vaccination. The golden algae at this time are taken out as the algal species of the present embodiment.

[0083] Step 2: Fermentation tank culture

[0084] (1) Prepare fermenter medium according to Table 2 (except ammonium chloride), calibrate the pH electrode, prepare 32 L per tank, fill 28 L, sterilize at 121 °C for 30 min. The mass ratio of carbon / nitrogen in the medium is 28:1.

[0085] (2) After the sterilization is completed, cool down, adjust the temperature to 28 ℃, rotate at 50 rpm, and ventilate for 4.0 L min -1 After that, c...

Embodiment 3

[0105] According to the culture method of Example 1, cultivate in a 7.5L fermenter, and set up the experimental group (tank 1 and tank 3) with vitamin addition and the control group (tank 2 and tank 4) without vitamin addition, as shown in the following table:

[0106]

[0107] The culture method, condition and operating steps of this embodiment are all carried out with reference to embodiment 1, and the experimental results are shown in image 3 . Depend on image 3 The statistical results show that:

[0108] ① by image 3 It can be seen that vitamin B was added to tank 1 and tank 3 at 44 h 1 : 0.375 mg / L, B H : 0.25mg / L, B 6 : 0.125 mg / L and B 12 : After 0.125 mg / L (vitamin concentration after adding to the culture system), the growth rate began to be significantly faster than tank 2 and tank 4. The fermenter of tank 1 has an average rate of 32.22×10 in 48-120 h 5 (one per mL / h), the average rate of tank 2 fermenter is 7.4×10 5 (each mL -1 h -1 ), tank 1 is 4....

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Abstract

The invention relates to a rapid culture method of chrysophyceae. The culture method is heterotrophic culture; a culture medium contains a carbon source and a nitrogen source; the carbon source is glucose; the nitrogen source is a mixed carbon source composed of an organic nitrogen source and an inorganic nitrogen source; the organic nitrogen source is selected from one or more of liver extract powder, beef extract, yeast extract powder and peptone; the inorganic nitrogen source is ammonium nitrogen or urea. In a culture process, a material supplementation culture medium is supplemented into afermentation tank; the temperature of a culture system in the fermentation tank is controlled to be 20 to 30 DEG C, the pH (Potential of Hydrogen) is 5.0 to 8.0, the dissolved oxygen concentration is10 to 60 percent and the concentration of glucose is stabilized to be 0 to 2g / L; a nitrogen-containing material supplementation culture medium is supplemented from the beginning of the culture to a first moment, and a nitrogen-free material supplementation culture medium is supplemented from the first moment to a second moment or the ending of the culture; a basic culture medium contains vitaminB1, BH, B6 or B12 or the vitamin B1, BH, B6 or B12 is added into the fermentation tank in a culture process. According to the method provided by the invention, after the culture is carried out for 96to 170h, the highest biomass can reach 56g / L and the growth of the chrysophyceae is remarkably accelerated after the vitamin B1, BH, B6 or B12 is added.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a rapid cultivation method for golden algae. Background technique [0002] Golden algae, also known as golden brown algae, are mostly distributed in fresh water and also in sea water. Golden algae cells are rich in nutrients, among which golden algae laminarose has a wide range of biological activities, and has a certain preventive effect on gout, hyperlipidemia, kidney disease, and early and middle stage renal failure; and the fucoxanthin contained in golden algae has multiple Biological effects, including weight loss, anti-oxidation, neuroprotection, anti-tumor, anti-inflammation, hypoglycemic / lipidemia, liver protection, anti-angiogenesis, eye protection and other pharmacological effects, so it is used as medicine, skin care and beauty products and health care products widely used in the market. Golden algae is also a good fish bait and has a wide range of use...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 陈剑平马明洋王谝龚迎春金虎胡强
Owner 德默特生物科技(珠海)有限公司
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