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Method for producing Chinese caterpillar fungus mycelium powder through fermentation

A technology for cordyceps sinensis mycelium and cordyceps sinensis fungus is applied in the field of fermentation and production of cordyceps sinensis mycelium powder, which can solve problems such as low mycelium yield, reduce production cost, solve low yield, save manpower and material resources and equipment occupancy rate Effect

Inactive Publication Date: 2019-02-19
马兰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the bottleneck of the industrial production of Cordyceps sinensis is mainly the lower mycelium yield

Method used

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  • Method for producing Chinese caterpillar fungus mycelium powder through fermentation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of slant seeds: DCH slant medium: 5.0% sucrose, 0.3% yeast powder, 1.0% tryptone, 1.0% agar powder, with an inorganic ion solution of pH 5.6-6.0 (0.5 grams of magnesium sulfate, 1 gram of potassium dihydrogen phosphate , 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate, and 1000 ml of distilled water). After sterilizing and cooling down, the activated Cordyceps sinensis strain is inoculated on the slant medium, and cultured at 15-18° C. for 35 days. The strain is selected from the Industrial Microorganism Culture Collection Management Center CICC, No. 14088.

[0037] Preparation of shake flask seeds: shake flask medium: glucose 1.0%, corn flour 2.5%, molasses 1.0%, potato 2.0%; live silkworm chrysalis 6.0%, soybean sprout powder 0.5%, yeast powder 0.2%, bran 1.0%; sulfuric acid Magnesium 0.01%, potassium dihydrogen phosphate 0.1%, and the rest is water...

Embodiment 2

[0045] Preparation of slant seeds: DCH slant medium: 5.0% sucrose, 0.3% yeast powder, 1.0% tryptone, 1.0% agar powder, with an inorganic ion solution of pH 5.6-6.0 (0.5 grams of magnesium sulfate, 1 gram of potassium dihydrogen phosphate , 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate, and 1000 ml of distilled water). The activated bacteria were inoculated on the slant medium and cultured at 15-18°C for 33 days. The strain is selected from the Industrial Microorganism Culture Collection Management Center CICC, No. 14088.

[0046] Preparation of shake flask seeds: shake flask medium: glucose 1.5%, corn flour 1.5%, molasses 1.5%, potato 3.0%; live silkworm chrysalis 4.0%, soybean sprout powder 1.0%, yeast powder 1.0%, bran 1.5%; sulfuric acid Magnesium 0.02%, potassium dihydrogen phosphate 0.05%, and the rest is water. First cut potatoes into small pieces, cut silkworm...

Embodiment 3

[0054] Preparation of slant seeds: DCH slant medium: 5.0% sucrose, 0.3% yeast powder, 1.0% tryptone, 1.0% agar powder, with an inorganic ion solution of pH 5.6-6.0 (0.5 grams of magnesium sulfate, 1 gram of potassium dihydrogen phosphate , 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate, and 1000 ml of distilled water). The activated bacteria were inoculated on the slant medium and cultured at 15-18°C for 34 days. The strain is selected from the Industrial Microorganism Culture Collection Management Center CICC, No. 14088.

[0055] Preparation of shake flask seeds: shake flask medium: glucose 1.5%, corn flour 2.0%, molasses 1.0%, potato 5.0%; live silkworm chrysalis 5.0%, soybean sprout powder 1.0%, yeast powder 0.4%, bran 1.5%; sulfuric acid Magnesium 0.01%, potassium dihydrogen phosphate 0.02%, and the rest is water. First cut potatoes into small pieces, cut silkworm...

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Abstract

The invention relates to the technical field of biological fermentation, and discloses a method for producing Chinese caterpillar fungus mycelium powder through fermentation. The method comprises thespecific steps that by means of slant strain culture, shake flask seed culture, first-stage seed tank and second-stage seed tank culture and fermentation tank culture, a mature fermentation solution is placed in tanks according to 80%-90% v / v of the fermentation volume, then a fermentation medium is added into the tanks until the original fermentation volume is reached, culture continues to be carried out, and 6-8 tanks are continuously cultured in the same way. According to the method, the defects that when the Chinese caterpillar fungus mycelium powder is produced through liquid fermentation, the period is long, and the yield is low are overcome, and the method is practical to industrialization of Chinese caterpillar fungus mycelium.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for fermenting and producing cordyceps sinensis mycelium powder. Background technique [0002] Cordyceps sinensis lives in alpine meadows at an altitude of 3000-5200 meters on the Qinghai-Tibet Plateau, and is a precious Chinese herbal medicine. Cordyceps sinensis is a complex of insects and bacteria formed after the larvae of Lepidoptera bat moths are parasitized by Ophiocordyceps sinesis. The relationship between Cordyceps sinensis and fungus is that after the Cordyceps fungus infects bat moth larvae, it gradually converts the nutrients in the larvae into mycelium to fill the entire insect body tissue, and keeps the insect shape unchanged for several months in the low-temperature and dry soil in winter (winter worm). When the temperature and humidity are suitable in summer, rod-shaped fruiting bodies (subseats) grow from the head of the insect body an...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 马兰陈大伟刘小朋
Owner 马兰
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