92 results about "Enramycin" patented technology

The invention relates to a preparation method of an enramycin premix, comprising the following steps of: carrying out high-pressure filtration / squeeze on enramycin fermentation liquor prepared by using a conventional method; separating the fermentation liquor; then carrying flash drying on an obtained enramycin filter cake and then crushing; adding a powdery carrier to fine mycelium powder according to the purity and the end-product requirement content of a dried product so as to regulate the concentration of the fine mycelium powder; evenly mixing to obtain a powdery enramycin premix; and pelleting the powdery enramycin premix into a granulated enramycin premix according to demands. The preparation process of the preparation method does not need to refine and extract enramycin, does not consume organic solvents and acid and base, and has less pollution on the environment, and the production cost is largely reduced; the content and the uniformity of the obtained product meet the quality standard requirements of imported veterinary drugs, and the stability of the product meets the specified requirements; moreover, dangerous solid wastes, such as antibiotic bacterium residues and the like, are not generated in the production and preparation process, thereby greatly reducing the pollution to the environment and the later-period management cost.

The invention provides a streptomyces fungicidious mutant strain FYFJ03 of which the collection number is CGMCC No.4113, the collection institution is China General Microbiological Culture Collection Center, and the collection date is August 25th, 2010. The invention also provides the application of the strain in the production of enramycin. In the mutant strain provided by the invention, threonine can be accumulated and the content of primer threonine for synthesizing the enramycin can be increased, so that the production level of the enramycin is improved.

The invention relates to compound feed for nutrient combo for milk pigs. The feed is prepared from the following raw materials: corn flour, expanded corn flour, whey powder, expanded soybean flour, peeled bean pulp flour, plasma protein flour, imported steam fish meal, cane sugar, fortide, zinc oxide, mountain flour, calcium hydrophosphate, L-Lysine hydrochloride, common salt, threonine, choline chloride, complex enzyme, enramycin, special premix compound M01 for MingWang pig farm, multidimensional D706 for pigs, antioxidant, sweetener, tryptophan, flavouring agent, bluestone, manganous sulfate, Zn-aminoacid, sodium selenite, calcinol, cobalt chloride, vitamin C powder and vitamin E powder. The compound feed has the advantages that the nutrition distribution is much more reasonable, the feeding effect is better, the compound feed can be conducive to the taste change and the physique enhancement of the milk pigs, enables the milk pigs to be large in size, to be uniform and to suffer less diseases, reduces the occurrence of yellow and white diarrhea and weaning syndrome, promotes the quick growth of the milk pigs, and can effectively increase the weight of weaning litter of the milk pigs.

The invention discloses a high-pressure liquid-phase preparation method for an enramycin standard sample, and the method comprises preparation of a fermented thallus test sample solution and preparation of a animal feed test sample solution, wherein the preparation of the fermented thallus test sample solution comprises the following steps of: suspending fermented thallus in an extraction solution, which is formed by mixing acetone, hydrochloric acid solution and water in a ratio of 20:1:21, in a ratio of 30-90 ml / g, disrupting cells by an ultrasonic disruptor or an ultrasonic machine to obtain an extraction solution containing enramycin, collecting a supernatant, filtering the supernatant by a filter membrane, and using the supernatant as the test sample solution for the future use; and the preparation of the animal feed test sample solution comprises the following steps of: performing reverse-phase high-performance liquid chromatography in corresponding conditions, collecting according to the retention time of the constituents, and then removing a solvent according to a conventional rotary evaporation process, thus obtaining the standard sample of the enramycin constituent. The high-pressure liquid-phase preparation method of the enramycin standard sample disclosed by the invention has the following advantages: 1, the pre-treatment is simple, fast, and easy to operate; 2, the collection method is simple; and 3, the purity of the prepared standard sample is high and up to more than 90%.

The invention provides enramycin dry suspension which contains 2 percent to 8 percent of enramycin, 20 percent to 60 percent of filler, 10 percent to 40 percent of flavoring, 5 percent to 30 percent of suspending aid and 1 percent to 10 percent of wetting agent. In addition, the invention provides a preparation method of the enramycin dry suspension. Compared with premix, when being used temporarily, the enramycin dry suspension can be dispersed into supensoid agent for oral taking only by being added with water and shaken; when being used, the enramycin dry suspension can become solution only by being added with water, the dispersion is uniform, and the defects when the premix is used are overcome; meanwhile, the specific surface area of the enramycin dry suspension is larger than that of the premix, the absorption is quick, the bioavailability is high and the cure rate is higher. Compared with the solution, the enramycin dry suspension is always in a dry state without water, so that the stability is more easily realized. In addition, the enramycin dry suspension is conveniently used, so that a lot of manpower and materials are saved. But a technology of preparing dry suspension with the enramycin is not yet reported.

The invention relates to a method for producing polypeptide antibiotics enramycin by the fermentation method, which pertains to the fields of agricultural biotechnology, industrial biotechnology and the fermentation engineering. The method is as follows: (1) slant culture: a Streptomyces sp.NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days; (2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days; (3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

The invention relates to a feed premix for yellow hair chickens. The feed premix is characterized by comprising the following raw materials in part by weight: 10-15 parts of composite vitamins, 100-120 parts of composite mineral substances, 125-150 parts of salt, 45-60 parts of choline chloride, 40-70 parts of prebiotics, 20-25 parts of coccidium-resistance drugs, 2-6 parts of complex enzyme, 25-60 parts of emulgator, 100-180 parts of lysine, 80-130 parts of methionine hydroxy analog-Ca, 10-20 parts of enramycin, 7-15 parts of antioxidants, 80-120 parts of lutein, 4-8 parts of canthaxanthin, and 50-350 parts of wheat middling. The feed premix has the advantages that the coloring of the yellow hair chickens is stable, and particularly in high-temperature summer, the good coloring of carcass can be ensured, and the coloring of feet diameters can reach more than 10 degrees of a Roche yolk color fan; a selected colorant mainly comprises the natural lutein extracted from marigold flowers, and according to the technology provided by the invention the application amount of the lutein and the canthaxanthin can be greatly reduced, thus playing a great role in protection of food safety; and the cost of pigments can be reduced greatly, and the competitiveness of yellow hair chicken production enterprises can be improved.

The invention discloses a strain for highly yielding enramycin, simultaneously provides a screening method of the strain, and provides application of the strain in enramycin production. The preserving number of the strain DCS067 for highly yielding enramycin is CGMCCONO: 6220, the preserving unit is China General Microbiological Culture Collection Center (CGMCC), and the preserving date is June 14, 2012. The DCS067 strain enables production rate of enramycin to be improved by 47.80%.

The invention discloses a method for reducing solvent use amount and extracting high-purity enramycin. The method comprises the following steps: performing heat treatment on enramycin fermentation liquor, filtering, centrifuging, and collecting wet mycelium; adding a methanol solution into the wet mycelium, regulating the PH, crushing, and collecting the liquor; regulating the PH of the liquor by using sodium hydroxide, adding a filter aid, performing suction filtration, leaching the filter residue, collecting the liquor and mixing with the filtrate; performing nanofiltration concentration and separation on the mixed solution, diluting the obtained concentrated solution, regulating the PH, filtering, and discoloring the filtrate; adsorbing the discolored solution by using a macroporous resin column, washing and eluting by using methanol or ethanol, collecting the eluant of the same substance, separating and purifying, concentrating and crystallizing, thereby obtaining the enramycin. By adoption of the method, the mycelium solution is crushed by utilizing a pulp refiner, the collected penetrating fluid subjected to nanofiltration concentration can replace deionized water and methanol for recycling, so that the methanol consumption is reduced, the production cost is reduced, the industrial wastewater is reduced, the high-purity enramycin is prepared, and the method is suitable for large-scale production.

The invention discloses a method for producing enramycin by fermentation. Cabicidin streptomyces avermitilis is used as a fermentation strain in the method; and water is supplied to the fermentation solution in the fermentation process of constant temperature culture in a fermentation medium, so that the dissolved oxygen quantity of the fermentation solution is improved, and the fermentation level of the enramycin is improved. The method is simple and feasible; and after a proper amount of water is supplied at proper time in the fermentation culture process, the fermentation level of the enramycin can be improved by about 130 percent.

The invention discloses a method for preparing enramycin premix. The method comprises the steps that the temperature of enramycin fermentation liquor is firstly increased to 70-75 DEG C, then complex antioxidants of which the mass accounts for 0.5-1.0% of that of the enramycin fermentation liquor and filter aid of which the mass accounts for 0-12% of that of the enramycin fermentation liquor are added, and the mixture is stirred fully and evenly; frame filter and flash drying are performed on the evenly stirred mixture, auxiliary materials are added in the mixture for dilution, and then the enramycin premix is prepared. The method is simple, easy to operate and low in cost, and compared with finished enramycin premix which is not preprocessed, the enramycin premix has the advantages that the degradation degree is obviously weakened and the stability is obviously improved.

The invention provides a high-performance liquid chromatography determination method of enramycin, comprising the following steps of: preparing a buffering salt solution; processing a sample; processing a standard product; feeding the sample; collecting a map and recording a peak area; processing data; calculating area and the like. The high-performance liquid chromatography determination method of the enramycin has the advantages that: 1: the sample processing is simple; 2: a detection step is simple and has low requirements on staff; and the method mainly depends on instrument analysis, simplicity and easiness of learning and easiness of popularization; 3: a detection period is short: the time for feeding and injecting the sample is 30 minutes and a result can be represented in the day of detecting; and 4: the relative deviation of the detected result is small: the method requires that the relative deviation of results of two times of parallel determination on the same sample by the same operator is not more than 2%.

The invention relates to a synthesizing method of a complete antigen of enramycin, belonging to the technical field of organic chemistry and immunochemistry. The synthesizing method comprises the following steps of: coupling the enramycin as a hapten with a carrier protein BSA (Bovine Serum Albumin) by using a glutaraldehyde method; and determining the coupling ratio of coupling substances by using a spectrophotometry. The invention successfully synthesizes an artificial antigen of the enramycin, adopts succinct and effective synthesizing step, is completely used for enzyme immnunoassay and provides a technological base for the fast detection, the easy and convenient operation and the cost reduction of related enzyme immnunoassay.

The invention relates to a new enramycin-producing strain and a high-yielding mutant strain thereof. In the invention, streptomyces obtained by separating marine environment is screened by using a primer designed by aiming at the conserved domains of lipopeptide antibiotics synthetic genes, and the new enramycin-producing strain of lipopeptide antibiotics is obtained through homology comparison. The invention also utilizes a ribosome engineering technology to breed mutant strains which has increased yield of enramycin. The invention provides a new strain source for fermenting and producing the enramycin, and the mutant strains can be used for industrial production.

The invention relates to a method for extracting enramycin. The method comprises the following steps of: soaking dried enramycin fungus dreg in an extracting agent; heating and refluxing; filtering; concentrating the filtrate under the vacuum condition until the filtrate is dried; dissolving the obtained substance; filtering; drying the filtrate by distillation under reduced pressure; dissolving the obtained solid substance; filtering; collecting filter cakes; and drying to obtain the enramycin. The method has simple steps; in the extracting process, all the organic solvents are easy to recycle; environmental pollution is light; and the obtained enramycin has high content and high reproducibility.

The invention provides a method for preparing an Enramycin A and B hydrochloride mixture. The method comprises the steps that: (1) a fermentation broth comprising Enramycin A and B is separated, such that mycelium is obtained; (2) an extraction liquid with pH regulated to 2.0-4.5 is mixed with the mycelium, wherein the pH is regulated by using hydrochloric acid; ultrasonic crushing is carried out for 1-3h; the mixture is stirred for 12-24h, such that a suspension liquid is obtained; (3) the suspension liquid obtained in the step (2) is sufficiently settled, and filtering is carried out, such that a crude extraction liquid comprising Enramycin A and B hydrochloride is obtained; (4) the crude extraction liquid obtained in the step (3) is subjected to reduced-pressure vacuum concentration under a temperature of 30-40 DEG C, until the volume of the liquid is 1 / 10-1 / 20 of an original volume, such that a concentrate liquid is obtained; (5) the pH of the concentrate liquid obtained in the step (4) is regulated to 8.0-9.0, and the Enramycin A and B hydrochloride is sufficiently dissolved; filtering is carried out, such that mother liquid for column processing is obtained; (6) the mother liquid obtained in the step (5) is processed by using macroporous absorption resin column, such that collected liquid comprising Enramycin A and B hydrochloride mixture is obtained; and (7) the collected liquid obtained in the step (6) is subjected to reduced-pressure vacuum concentration, such that the volume of the liquid is 1 / 50-1 / 100 of an original volume; and lyophilization is carried out, such that the Enramycin A and B hydrochloride mixture is obtained.

The invention discloses a preparation method of an enramycin premix. The method comprises the specific steps that: an eramycin fermentation broth is subjected to plate frame pres filtration, flash drying, and crushing; a carrier is added, and the mixture is well mixed; and granulation, drying, and sieving are carried out, such that the enramycin premix is obtained. According to the invention, granulation rate and yield of the obtained eramycin premix are greatly improved, titer stability is substantially improved, and effective component wasting is reduced. The process is simple to operate, and cost is greatly reduced. The method is suitable for industrial production, and is environment-friendly.

The invention relates to a method for fermenting enramycin. According to the method, one or more amino acids, such as arginine, phenylalanine, aspartic acid, threonine and lysine, and salts of the amino acid(s) are added into an enramycin fermentation liquid. The method can be used for greatly increasing the yield of enramycin, and is applicable to industrial production.

The invention discloses a method of improving stability of a finished enramycin premixing agent. The method comprises the steps: after the fermentation cultivation of enramycin is finished, inactivating fermentation broth at high temperature, cooling and respectively performing pre-treatment according to chitosan solution and sodium polyacrylate solution; and performing plate-frame filtering and spray drying to the processed fermentation broth to obtain the finished premixing agent. Compared with the present enramycin production technology, the fermentation broth is pre-treated and the drying manner is adjusted, so that the quality of the finished enramycin is changed. The method of improving stability of the finished enramycin premixing agent is strong in operability and low in cost; compared with the non-pretreated finished products or the finished products pre-treated in the other manners, the finished enramycin premixing agent obtained by the method provided by the invention is non-degradable basically and the stability of the finished product is increased during storage. The stability problems of the finished enramycin premixing agent during transportation, storage and usage are solved.

The invention relates to the technical field of veterinary drugs, and aims to provide a micro-emulsion containing enramycin and a preparation method thereof. The micro-emulsion containing enramycin is a water-in-oil type micro-emulsion formed by mixing enramycin micro-emulsion concentrate and water in a volume ratio of (1:50)-(1:1000). The method for preparing the micro-emulsion containing enramycin comprises the following steps: mixing a surfactant and an oil phase to obtain a mixed liquid a, adding enramycin into a co-surfactant, mixing to obtain a mixed liquid b, and mixing the mixed liquid a and the mixed liquid b to obtain the enramycin micro-emulsion concentrate; dropwise adding the concentrate into water, and stirring to obtain the micro-emulsion containing enramycin. According to the micro-emulsion, the water-in-oil micro-emulsion is adopted as a carrier of a hydrophobic medicine at good drug dispersion, is an ideal carrier of insoluble medicines, can be used for prolonging the releasing time of water soluble medicines to play a sustained release effect. The preparation method is simple in production process, convenient to prepare and low in cost.

The invention discloses an optimized process for producing enramycin through fermenting streptomyces. In the method, the formation of the enramycin is promoted through optimizing components of a culture medium, adding inorganic salt and low-molecular active substances (amino acid), disturbing the metabolic network of the streptomyces, and enhancing a formation approach of the target product; and the culture process is optimized through transforming the fermentation process and adopting a feed culture method. The optimized process for the fermentation of the enramycin, provided by the invention, is mainly used for greatly improving the fermentation potency of the enramycin and increasing the utilization rate of equipment and raw materials through optimizing the components of the culture medium and the feed process.

A creep feed is a special high-nutrition feed for feeding suckling pigs in one week after the suckling pigs are born and in 1-2 weeks after the suckling pigs are weaned, and the creep feed is commonly known as a milk replacer or artificial milk and is a feeder prepared for replacing full milk. Main raw materials of the creep feed are byproducts of the milk industry. The invention relates to a creep feed which consists of extruded corn, fermented soybean meal, whey powder, whey protein meal, imported steamed fish meal, cane sugar, membrane protein meal, plasma protein powder, plant essential oil, compound vitamins, composite amino acids, mineral substances, salt, colistin sulfate, enramycin, sodium selenite, a flavoring agent, an acidifying agent, probiotics and the like. According to the creep feed, animal protein raw materials are used as much as possible, the fermented soybean meal is used for replacing conventional soybean meal, the extruded corn is used for replacing conventional corn and simultaneously, the acidifying agent and the probiotics are added, so anti-nutritional factors in the raw materials are farthest reduced, the palatability and digestibility of the feed are improved, the appetite of the suckling pigs is stimulated, food intake is increased, growth of intestinal tracts is promoted, immunity is enhanced, the diarrhea rate of the suckling pigs in a creep stage is reduced, the survival rate of nursery pigs is increased, weaning stress is reduced and finally, targets of successful creeping and increment of daily gain are achieved.

The invention discloses an ecologic and nutritive crab feed. The ecologic and nutritive crab feed is prepared by using the following raw materials, by weight, 18-20 parts of soybean protein peptide, 1-2 parts of Coriander Leaf essential oil, 5-8 parts of mushroom seasoning oil, 2-4 parts of ethyl maltol, 2.1-3.2 parts of gelatin, 14-17 parts of betel nut, 2-4 parts of an irritation agent, 0.3-0.5 parts of enramycin, 19-23 parts of shredded squid, 16-18 parts of preserved apricot, 16-21 parts of silver carp meat, 15-17 parts of orange, 5-8 parts of lemon, 8-10 parts of bee pollen, 0.3-0.5 parts of Radix Astragali, 0.2-0.4 parts of broadleaf holly leaf, 0.1-0.4 parts of Rhizoma Atractylodis Macrocephalae, 3-6 parts of an attractant, and a proper amount of water. The ecologic and nutritive crab feed has the advantages of reduction of the pollution to culturing environment, attraction of crab feeding, improvement of the irritability of crabs, fast growth and the like.

The invention discloses a method for producing enramycin by fermentation. Cabicidin streptomyces avermitilis is used as a fermentation strain in the method; and water is supplied to the fermentation solution in the fermentation process of constant temperature culture in a fermentation medium, so that the dissolved oxygen quantity of the fermentation solution is improved, and the fermentation level of the enramycin is improved. The method is simple and feasible; and after a proper amount of water is supplied at proper time in the fermentation culture process, the fermentation level of the enramycin can be improved by about 130 percent.

The invention relates to a method for producing enramycin by fermenting. According to the method, bactericidin streptomycete FYFJ03 is taken as a fermenting strain; during a fermenting process for constant temperature culturing, different sugar concentrations are fed under different stages; the dissolved oxygen of fermentation liquor is improved; and the fermentation level of the enramycin is increased. The method is simple and easy to carry out. After the fermentation is performed according to the method during the fermenting culturing process, the fermentation level of the enramycin is increased by about 20%.

The invention relates to a method for detecting enramycin residue in a meat product, belonging to the technical field of food detection. The invention aims at providing a low-detection limit method for detecting enramycin residue in the meat product. The method for detecting enramycin residue in a meat product comprises the following steps: a. respectively adding an extracting solution of a meat product to be detected and an enramycin standard solution with series concentration into micropores of a pre-coated enramycin antigen; b. adding an enramycin antibody into each micropore, uniformly mixing and incubating; c. adding horse radish peroxidase HRP-goat anti-rabbit (IgG) into each micropore, uniformly mixing and incubating; d. respectively adding a substrate solution into each micropore, and developing; e. respectively adding a stopping solution into each micropore to stop the reaction, and reading an OD450 value of each pore by using a microplate reader; and f. contrasting a curve regression equation obtained by the enramycin standard solution, and calculating the enramycin content in the meat product to be detected.

The invention relates to a method for breeding a high-yield enramycin strain. The method comprises the following steps: determining the short gene of a rate-limiting enzyme coding gene in the enramycin biological synthesizing process; constructing a thiostrepton resistance gene integrating vector; mutagenizing the modified strains, and screening a thiostrepton high-resistance strain from the modified strains; and carrying out genetic manipulation on the screened high-resistance strain, and restoring the tsr open reading frame to the original endX gene to obtain the strain which is an enramycinhigh-yield mutant strain. The method combines advantages of a traditional mutation breeding technology and a modern molecular biological method to realize quick and efficient screening of the enramycin high-yield strain. The method is applicable to screening of other antibiotic high-yield strains.

The invention relates to an artificially-synthesized vitreoscilla hemoglobin gene. The nucleotide sequence of the gene is shown in SEQ ID No.1, and the gene is marked as vgbL. The invention further provides a corresponding genetically-engineered strain for producing enramycin. The invention further provides a method for constructing the genetically-engineered strain for producing the enramycin. The artificially-synthesized vitreoscilla hemoglobin gene, the strain and the method have the advantages that the nucleotide sequence of the novel vitreoscilla hemoglobin gene vgbL which can be efficiently expressed in streptomyces is obtained, and the gene is artificially synthesized and transferred into fungicidal streptomyces ATCC21013; compared with an original wild strain, by means of the genetically-engineered strain, the yield of the enramycin is increased by 16.6%, and therefore, it can be seen that the vgbL can become an effective means of increasing the yield of antibiotics in variousstreptomyces.

The invention provides a purifying method of enramycin. The purifying method comprises the steps of regulating the pH value of a flash powder mixture of methanol and enramycin by using hydrochloric acid to be acidic to obtain an enramycin extracting solution; then, regulating the pH value of the enramycin extracting solution to be neutral, and sequentially eluting by taking column-layer chromatographic silica gel as a separating medium and taking petroleum ether, ethyl acetate, butyl acetate and methanol as eluants to remove impurities; next, eluting enramycin by using a methanol solution of hydrochloric acid to obtain an eluant containing enramycin; and carrying out rotary evaporation and drying after regulating the pH value of the eluant containing enramycin to be neutral to obtain enramycin. By using the method provided by the invention, the impurities can be effectively removed, and defects in the existing purifying method of enramycin can be overcome; experiments prove that the content of enramycin can be up to more than 98% if enramycin is prepared by using the method provided by the invention.

The invention relates to the field of processing of feed drugs, in particular to a preparation method of an enramycin premixing agent. The preparation method comprises the following steps of filtering an enramycinfermenting liquid by a plate frame; performing flash evaporation and drying on an obtained filter cake, so as to obtain enramycin mycelium; diluting the mycelium and auxiliary material according to ratio, and sufficiently stirring; uniformly stirring the mycelium diluted liquid, and drying, so as to obtain the enramycin premixing agent, wherein the weight ratio of enramycin mycelium and auxiliary material is 4 to 6, and the added auxiliary material is glycerides. The enramycin premixing agent prepared by the preparation method has the advantages that the degrading degree of the particle finished product is obviously decreased, and the stability is obviously improved.