Method for reducing solvent use amount and extracting high-purity enramycin

Abstract

The invention discloses a method for reducing solvent use amount and extracting high-purity enramycin. The method comprises the following steps: performing heat treatment on enramycin fermentation liquor, filtering, centrifuging, and collecting wet mycelium; adding a methanol solution into the wet mycelium, regulating the PH, crushing, and collecting the liquor; regulating the PH of the liquor by using sodium hydroxide, adding a filter aid, performing suction filtration, leaching the filter residue, collecting the liquor and mixing with the filtrate; performing nanofiltration concentration and separation on the mixed solution, diluting the obtained concentrated solution, regulating the PH, filtering, and discoloring the filtrate; adsorbing the discolored solution by using a macroporous resin column, washing and eluting by using methanol or ethanol, collecting the eluant of the same substance, separating and purifying, concentrating and crystallizing, thereby obtaining the enramycin. By adoption of the method, the mycelium solution is crushed by utilizing a pulp refiner, the collected penetrating fluid subjected to nanofiltration concentration can replace deionized water and methanol for recycling, so that the methanol consumption is reduced, the production cost is reduced, the industrial wastewater is reduced, the high-purity enramycin is prepared, and the method is suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for extracting and purifying enramycin, in particular to a method for extracting high-purity enramycin which reduces the use of solvents. Background technique [0002] Enramycin is an organic base composed of 17 amino acid molecules and fatty acid molecules of 13 different types. Among them, the amino acid molecule constitutes a cyclic polypeptide structure, and the fatty acid is located at the end of the polypeptide structure. According to the different types of terminal fatty acids, it is divided into enramycin A and enramycin B, and enramycin is a mixture of these two components. Enramycin hydrochloride is a white crystalline powder with a molecular weight of about 2500 and a melting point of 238-245°C. It is easily soluble in dimethyl sulfoxide, soluble in methanol and aqueous ethanol, hardly soluble in acetone, and insoluble in benzene. , Chloroform. [0003] Enramycin has high stability in feed and can be stor...

AI Technical Summary

Problems solved by technology

However, in the traditional technology, enramycin is extracted by immersion extraction and methanol solution as a solvent, the product yield is between 85.0% and 87.0%, and the product purity is between 97.2% and 98.5%.

It can be seen that the yield and purity of enramycin products are relatively low, and a large amount of methanol solution needs to be used in production, the production cost is high, and the environment is also affected to a certain extent.