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Method for extracting enramycin

An extraction method and technology of enomycin, applied in the field of feed pharmaceutical additive manufacturing, can solve the problems of complicated operation, poor reproducibility, large product loss, etc., and achieve the effects of simple preparation process steps, good product stability, and little environmental pollution

Active Publication Date: 2012-03-28
浙江拜克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of complex operation, large product loss and poor reproducibility in the prior art, the present invention provides an extraction method of enmycin with simple process steps, low production cost, little environmental pollution and good reproducibility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Leaching

[0035] ① Soak 250g of dried entomycin residue in 500mL extractant for 20min. The extractant used is an acidic aqueous solution composed of 200mL ethanol and hydrochloric acid with a pH of 4.0. Stir and reflux at 50°C for 1hr, then filter;

[0036] ② Soak the filter cake in step ① with the same amount of the same extractant as in step ① for 30 minutes, stir and reflux at 50°C for 1 hour, and then filter;

[0037] 3. The filtrate in step 1. and 2. is combined to obtain the extract, and the total yield of the extraction step is 98.3%;

[0038] (2) concentrated

[0039] Concentrate the extract collected in step (1) to dryness under vacuum at 60°C;

[0040] (3) dissolve

[0041] Dissolve the solid obtained in step (2) in 250mL ethanol, and filter;

[0042] (4) concentrated

[0043] Concentrate the filtrate in step (3) to dryness in vacuum at 50°C;

[0044] (5) Water extraction

[0045] Dissolve the dry matter in step (4) in 250mL of water, f...

Embodiment 2

[0047] (1) Leaching

[0048] ① Soak 250g of dried entomycin residue in 2500mL extractant for 40min. The extractant used is an acidic aqueous solution composed of 1500mL methanol and sulfuric acid. The pH is 5.0. Stir and reflux at 80°C for 4hr, then filter;

[0049] ② Soak the filter cake in step ① with the same amount of the same extractant as in step ① for 30 minutes, stir and reflux at 80°C for 4 hours, and then filter;

[0050] ③ The filtrate in steps 1. and 2. is combined to obtain an extract, and the total yield of the extraction step is 98.5%;

[0051] (2) concentrated

[0052] Concentrating the extract in step (1) in vacuum at 80°C to dryness;

[0053] (3) dissolve

[0054] Dissolve the solid substance obtained in step (2) in 1250mL methanol, and filter;

[0055] (4) concentrated

[0056] Concentrate the filtrate in step (3) to dryness in vacuum at 70°C;

[0057] (5) Water extraction

[0058] Dissolve the dry matter in step (4) in 500mL of water, filt...

Embodiment 3

[0060] (1) Leaching

[0061] ① Soak 250g of dried entomycin residue in 1000mL extractant for 30min. The extractant used is an acidic aqueous solution composed of 500mL acetone and acetic acid. The pH is 6.0. Stir and reflux at 75°C for 2hr, then filter ;

[0062] ② Soak the filter cake obtained in step ① with the same amount of the same extractant as in step ① for 30 minutes, stir and reflux at 75°C for 2 hours, and then filter;

[0063] ③ The filtrate in steps 1. and 2. is combined to obtain an extract, and the total yield of the extraction step is 98.0%;

[0064] (2) concentrated

[0065] The extract collected in step (1) was concentrated and evaporated to dryness in vacuo at 70°C;

[0066] (3) dissolve

[0067] Dissolve the solid obtained in step (2) with 750mL methanol, and filter;

[0068] (4) concentrated

[0069] Concentrate the filtrate in step (3) in vacuo at 60°C and evaporate to dryness;

[0070] (5) Water extraction

[0071] Dissolve the dry su...

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PUM

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Abstract

The invention relates to a method for extracting enramycin. The method comprises the following steps of: soaking dried enramycin fungus dreg in an extracting agent; heating and refluxing; filtering; concentrating the filtrate under the vacuum condition until the filtrate is dried; dissolving the obtained substance; filtering; drying the filtrate by distillation under reduced pressure; dissolving the obtained solid substance; filtering; collecting filter cakes; and drying to obtain the enramycin. The method has simple steps; in the extracting process, all the organic solvents are easy to recycle; environmental pollution is light; and the obtained enramycin has high content and high reproducibility.

Description

technical field [0001] The invention relates to the field of feed drug additive manufacturing, in particular to an extraction method of enmycin. technical background [0002] Enramycin (Enramycin), also known as Enramycin, Anlemycin, and Duramycin, is a polypeptide antibiotic, fermented by actinomycetes, and is an organic base composed of amino acid molecules and fatty acid molecules. Among them, amino acid molecules form a cyclic polypeptide structure, and fatty acid molecules are located at the end of the polypeptide structure. According to the different terminal fatty acid molecules, it is divided into enmycin A (C 107 h 138 Cl 2 N 26 o 31 ), entomycin B (C 108 h 141 Cl 2 N 26 o 31 ) two components, enmycin is the mixture of these two components. Enmycin is easily soluble in dilute hydrochloric acid and dimethylformamide, soluble in methanol and ethanol, insoluble in acetone, insoluble in acetic acid, benzene, and chloroform, and is a new type of broad-spectrum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/14
Inventor 生英涛储消和黄海涛唐俊李大娟
Owner 浙江拜克生物科技有限公司
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