125results about How to "Increase multiple" patented technology

A laser scanning system for generating a laser scanning pattern in a scanning field, while amplifying the scan-angle multiplication factor of rotating mirrors employed therein. The laser scanning system employs rotatable laser scanning assembly having an axis of rotation and first and second rotating mirrors with normal vectors that are coplanar with each other and said rotational axis, and which form an acute angle substantially less than 90 degrees so as to provide a laser scanning assembly with a scan angle multiplication factor that is greater than 2.0. A cluster of stationary mirrors mounted about the first and second rotating mirrors, for sweeping a laser beam off the cluster of stationary mirrors after a laser beam has been reflected off the first rotating mirror, then reflected off the second rotating mirror, and then directed outwardly towards an array of pattern mirrors, so as to generate a resultant laser scanning pattern within the scanning field.

The protective film includes a transparent supporting substrate, and a protective layer that is formed on a surface of the supporting substrate and contains fluorine. The protective layer has an uneven surface structure obtained by periodically and continuously forming recesses and projections and the uneven surface structure satisfies relations of t≦390 / nb (nm) and h / t ≦0.4. Herein, t represents a period (nm) of the uneven surface structure, h represents a height (nm) which is a distance between a top of each of the projections and a bottom of each of the recesses, and nb represents a refractive index of the protective layer.

A high-sensitivity laminated type flexoelectric pressure sensor comprises a metal elastic element for measuring mechanical deformation caused by micro-pressure. A plurality of flexoelectric dielectric films are attached to the top of the metal elastic element. The upper surface of each flexoelectric dielectric film and the lower surface of the flexoelectric dielectric film are provided with an upper metal electrode and a lower metal electrode respectively, each upper metal electrode and the corresponding lower metal electrode are separated through an insulating layer, the upper metal electrode of each flexoelectric dielectric film and the lower metal electrode of the flexoelectric dielectric film are respectively connected with a lead for outputting a measured load signal, each flexoelectric dielectric film together with the upper metal electrode, the lower metal electrode and the insulating layer is arranged in a shell, one end of each lead is led out of the shell, an insulating gasket is arranged between the metal elastic element and the shell, and a pressure channel for executing pressure is arranged at the lower portion of the metal elastic element. According to the high-sensitivity laminated type flexoelectric pressure sensor, pressure exerted on the films is measured through the linear relation between the exerted pressure and the stress gradient of mechanical deformation in the metal elastic element, output loads are improved through laminating, sensitivity is improved, and pressure can be accurately and easily measured.

The invention discloses a lymphocyte culture fluid and culture method thereof and application therefor, the culture fluid is adding ATP and coenzyme A in completely culture fluid containing basic culture fluid RPMI-1640, albumin, L-glutamine, glucose, baking soda and HEPES. The culture method includes steps: (1) preparing culture fluid; (2) suspending cell in culture fluid prepared by step (1); (3) activating cell; (4) washing activated cell by the culture fluid prepared by step (1); (5) collecting cell. The culture fluid of the invention cultures cell with higher amplification times and longer generation time.

Automotive vehicle including a radar transceiver each including a heterodyne active IMPATT multiplier module arranged to receive a signal from a VCO, a first balance mixer arranged to receive a signal from a VCO, a second balance mixer arranged to receive a signal from a receive antenna and the IMPATT multiplier module and derive a first intermediate frequency signal, a first amplifier for amplifying the output of the second balance mixer and providing the amplifier output to the first balance mixer, and a second amplifier for amplifying the output of the first balance mixer. The vehicle also includes a processor which receives output from the second amplifier of each transceiver and generates a control signal for controlling one or more vehicular components based on the output from the second amplifier(s). The components can be part of a collision avoidance system, blind spot monitoring system and the like.

A ranging apparatus 1 comprises a laser light emitter 3 for emitting pulsed laser light, a reflected light receiver 4 for receiving reflected light, a distance computer 10 for finding the distance from the elapsed time until the reflected light is received, and a distance display 8 for displaying this distance. The distance computer 10 has a counter 11 for counting the frequency when the reflected light satisfies a specific condition, a table production component 12 for producing a frequency distribution table corresponding to distance by adding up the counts, a distance determiner 13 for determining as the distance to the object of measurement the point when the frequencies in the frequency distribution table exceed a threshold, and a distance selector 15 for selecting a specific distance when a plurality of distances are determined, and displaying this distance on the distance display 8. As a result, when a plurality of distances are calculated, these distances can be appropriately displayed, improving the flexibility and functionality.

The invention relates to the field of gene engineering and cytobiology, in particular to a preparation method for efficiently amplifying NK cells by utilizing trophoblasts. The construction method comprises the following steps: (1) constructing a pHR-mbIL-21 plasmid vector, a pHR-4-1BBL plasmid vector and a pHR-MICA plasmid vector; (2) preparing recombinant lentiviruses by using pHR-mbIL-21, pHR-4-1BBL and pHR-MICA plasmid vectors respectively; (3) preparing K562-mbIL-21-4-1BBL-MICA trophoblasts; (4) extracting PBMC cells; and (5) carrying out in-vitro amplification on NK cells. The inventionprovides the preparation method of NK cells. The method employs the step of independently constructing plasmid vectors for expressing mbIL-21, 4-1BBL and MICA molecules, K562 is infected with recombinant lentivirus, the NK cells can be prepared, the preparation method overcomes the defects in the prior art, the K562 cells simultaneously expressed by IL-21, 4-1BBL and MICA molecules are used as trophoblasts for amplification culture of the NK cells, the amplification multiple of the NK cells reaches up to 890 times, the purity of the prepared NK cells reaches 92.2%, and the repeatability of theamplification multiple between different PBMC cells is good.

The invention discloses a preparation method of CIK (cytokine-induced killer) cells. The preparation method includes the steps of 1, separating single nuclear cells from blood; 2, mixing single nuclear cells suspension with fibrin gel, disposing a mixture in a CD3McAb and RetroNectin pre-coated culture bottle, and adding a medium containing PPP and IFN-Gamma; 3, on a second day, adding IL-1Alpha and IL-2 for continual culturing, stopping culturing until the single nuclear cells are converted into CIK cells. The preparation method has the advantages that the fibrin gel is a polymer network system capable of absorbing a great amount of water and extremely similar to extracellular matrix in structure, an in-vivo cell growth microenvironment can be simulated so that sufficient growth space for cells and a spatial structure supporting inter-cell connection are provided, amplification times of the cells are significantly increased, and the obtained cells are more active.

The invention relates to the technical field of biomedical science, in particular to a method for increasing the yield of immune cells. The method for increasing the yield of the immune cells comprises the steps of dilution of human peripheral blood and separation, frozen preservation, resuscitation and induction and amplification culture of the immune cells. By extracting and preserving healthy cells and conducting induction and amplification culture at a later stage, the yield of the immune cells is greatly increased; by means of a specific cell frozen preservation solution adopted in the frozen preservation process, the activity of the immune cells can be effectively maintained; laminarin is added in the induction and amplification culture process, the cells which are high in amplification times and higher in purity can be obtained, and therefore the yield of the immune cells is significantly increased.

The invention relates to the biotechnology field, in particular to CIK cell culture fluid, a CIK cell culture method and application of lentinan in CIK cell culture. The CIK cell culture fluid comprises interferon-gamma, CD3 stimulated monoclonal antibodies, interleukin-2, the lentinan and serum-free basal culture media. When the CIK cell culture fluid with the lentinan is used for CIK cell culture, PBMCs (peripheral blood mononuclear cells) can generate lymphocyte activation factors and release helper T cell factors to promote CIK cell multiplication, so that obtained CIK cells have large amplification multiple, high killing activity and high cell surface antigen content. Experiment results show that after 14-day CIK cell culture by the CIK cell culture fluid, the amplification multiple of the CIK cells is larger than 300, in-vitro killing rate (effector-target ratio being 40:1) of the CIK cells is larger than (80+ / -2)%, and the number of CIK cell surface antigens (CD3+ and CD56+) is larger than 48%.