The invention provides a culture
system and a culture method for efficient amplification
in vitro of autologous NK (Natural Killer) cells. The culture
system comprises a basic culture medium containing GT-T551,
interleukin IL-2,
interleukin 1L-12,
interleukin IL-15, interleukin IL-18, interleukin 1L-17, interleukin IL-22 and natural
traditional Chinese medicine substances. The culture method comprises the following steps of: collecting and separating autologous 40ml
peripheral blood mononuclear cells; resuspending the cells by a culture medium containing 10 percent of self
plasma; adding the cells into a
cell culture
bottle coated by CD3 antibodies or CD56 antibodiees; performing stimulation by a
cell factor combination A; putting the cells in a 37 DEG C and 5-percent-CO<2> culture tank to perform incubation and shaking; after 6 to 7 days, transferring the cells into a
cell culture bag to be cultured; complementally adding a culture medium containing a cell factor combination B; in the culture period, culturing the cells in a basic culture medium containing IL-2 every 3 to 4 days; and obtaining high-purity NK cells after the total culture time of 17 to 19 days. The culture
system and the culture method have the advantages that the NK cells can be amplified by more than 1000 times
in vitro; and the high-purity NK cells exceeding 5*10<9> can be obtained.