The invention discloses a high-affinity human
monoclonal antibody against
yellow fever virus and application thereof, and belongs to the technical field of medicines. The high-affinity human
monoclonal antibody against
yellow fever virus is prepared by the following steps: taking E
protein of
yellow fever virus expressed in
Escherichia coli as an
antigen; screening memory B cells which can specifically bind to the E
protein of yellow fever virus from PBMCs of a convalescent patient by performing
flow sorting, thereby further obtaining variable-region fragments of an
antibody; connecting the variable-region fragments of the antibody with a
constant region to an
expression vector; carrying out expression in mammalian cells, and carrying out purification; and then, carrying out a series of function tests, thereby obtaining 1 strain of the high-affinity human
monoclonal antibody against yellow fever virus. The affinity between the antibody and the
antigen is 1.49pm; moreover, the antibodyhas very strong yellow fever
virus neutralization activity and IC50 at 4ng / ml. In addition, the antibody is capable of completely protecting mice from yellow fever virus at
lethal dose. Thus, the high-affinity human
monoclonal antibody against yellow fever virus has application values in
clinical treatment and prevention of yellow fever virus.