53 results about "Flow sorting" patented technology

Many areas of biomedical research depend on the analysis of uncommon variations in individual genes or transcripts. Here we describe a method that can quantify such variation at a scale and ease heretofore unattainable. Each DNA molecule in a collection of such molecules is converted into a single particle to which thousands of copies of DNA identical in sequence to the original are bound. This population of beads then corresponds to a one-to-one representation of the starting DNA molecules. Variation within the original population of DNA molecules can then be simply assessed by counting fluorescently-labeled particles via flow cytometry. Millions of individual DNA molecules can be assessed in this fashion with standard laboratory equipment. Moreover, specific variants can be isolated by flow sorting and employed for further experimentation. This approach can be used for the identification and quantification of rare mutations as well as to study variations in gene sequences or transcripts in specific populations or tissues.

This invention relates to a high-speed network service flow sorting method, which is suitable for network flow monitor and analysis based on service flow and can automatically detect new service flows based on the service flow definition rule and the actual network flow. The method applies three stages of searching: 1, applying Hash method, scattering distribution of flow recording as far as possible, 2, providing two ways to avoid the Hash conflict, one is linear link list, the other is a searching tree, 3, linear searching and recording refreshment.

The invention relates to a robustness sorting learning method based on multi-objective particle swarm optimization and an application thereof, and the method comprises the following steps of 1, designing an effective deviation function and a robustness variance function of a sorting model based on a deviation-variance equalization theory, and constructing two optimization performance indexes of sorting learning; step 2, on the basis of a multi-objective particle swarm optimization algorithm framework, iteratively optimizing the two objectives of the effectiveness deviation function and the robustness variance function of the sorting model on the sorting learning data set to train the sorting model so as to generate a sorting model archiving solution set; and step 3, selecting a Pareto optimal sorting model with a maximum net flow sorting value from the sorting model filing solution set generated in the previous step as a trained final sorting model based on the idea of a preference sequence structure assessment method PROMEHEE II in a multi-attribute decision theory. Compared with the prior art, the method has the advantages of improving the overall user satisfaction, enhancing theuser experience and the like.

The invention discloses a human monocolonal antibody with high neutralization activity for Zika virus and application thereof, and belongs to the technical field of medicines. Escherichia coli-expressed Zika E protein is taken as an antigen, a memory B cell capable of specifically binding the Zika E protein is screened from PBMCs of a Zika patient in convalescence through flow sorting, then the screened single B cell is subjected to RT-PCR to obtain a variable region sequence and fragment of the antibody, and the variable region sequence and fragment of the antibody are further connected to anexpression vector together with a constant region. After the expression and purification of mammalian cells, a serious of function tests including binding force with ZIKE-E protein, in-vitro neutralization effect, in-vivo protective capability and the like are carried out, and 3 strains of the human monocolonal antibody with full protection against Zika virus infection are obtained.

The invention provides a flow sorting device for cell beads, and particularly provides a magnetic field generation device. The magnetic field generation device comprises a magnetic generation unit, a fixing device and magnetic noise eliminating assemblies, wherein the fixing device is used for fixing the magnetic generation unit; the magnetic noise eliminating assemblies are arranged at two ends of the fixing device; the magnetic generation unit comprises four magnetic assemblies which are arranged along the same axis; the four magnetic assemblies are fixed to be opposite each other two by two by the fixing device; the four magnetic assemblies surround to form a channel through which a pipeline is permitted to pass; in the magnetic assemblies, the magnetic poles of the opposite surfaces of the two opposite magnetic assemblies are N poles; and the magnetic poles of the opposite surfaces of the other two opposite magnetic assemblies are S poles. The invention further provides a method for sorting cells or separating target molecules on the basis of the magnetic field generation device. By the device and the method provided by the invention, efficient, reliable and high-flux cell sorting and enriching and target molecules separating can be achieved.

The invention discloses a network data acquisition system and a network data acquisition method. The network data acquisition system comprises an interface adaption module, a flow sorting and load balancing module, a protocol decoding and event message aggregation module, a multi-interface event data association module, and a controller module. By adopting the technical scheme provided by the invention, the problems of the prior art such as rigidity and inflexibility of the data acquisition method of the data acquisition system can be solved, and the configurability and the customization of the data acquisition can be realized, and at the same time, the flexible deployment of the data acquisition system networking can be realized.

A port monitor monitors network traffic that passes through a port of a switch. Frame flows routed through the switch are counted by hardware frame counting logic, which includes a content-addressable memory (CAM) and counters corresponding to various frame flows. Port monitor software includes logical containers (“buckets”), which are used to record and sort counts of the frame flows from hardware based on activity of the frame flows. Frame flow sorting is based on confidence building algorithms such that the thrashing of frame flows between buckets is reduced. Storage and sorting of the frame flows in software allows the activity level of any number of frame flows associated with the switch to be counted, regardless of hardware limitations on how many frame flows can be counted. This allows a real-time analysis of frame flows and a determination of frame flows that are major users of Fibre Channel bandwidth.

The present invention relates to a system and method of genomic profiling and is particularly useful in genomic differentiation of heterogeneous and polyclonal neoplastic cell populations, preferably of flow sorted formalin fixed paraffin embedded samples. The present invention includes methods of improving resolution for identifying aberration in variable carcinoma genomes and / or heterogeneous cell populations. The present invention also includes kits configured to improve genomic resolution and the ability to identify genomic aberration in variable and / or heterogeneous cell populations.

The invention discloses a construction method and an application of an NK (natural killer) cell activation testing receptor signal path NKG2D fluorescence report system. A human NKG2D extracellular region gene sequence and mouse NKG2D transmembrane region and cytoplasmic region gene sequences are obtained by the aid of PCR (polymerase chain reaction) technology, the human and mouse NKG2D gene sequences are connected together by the aid of overlapping PCR technology, and the constructed human and mouse heterozygous NKG2D sequences are inserted into a pLXSN16E6E7 vector to form a vector pLXSN16E6E7-mhNKG2D. A human DAP12 gene sequence is constructed by the aid of the PCR technology and inserted into the pLXSN16E6E7 vector to form a vector pLXSN16E6E7-hDAP12. Plasmids of the two vectors and helper plasmids pCL-ECO of a retrovirus system are co-transfected with HEK293T cells to obtain virus particles containing reinfected mouse cells 3A9. Cell supernatants containing virus particles infect the cells 3A9, a cell line 12A9 steadily expressing mouse and human infusion NKG2D and human DAP12 is screened out by means of repeated flow sorting and re-culture, and downstream signals of NKG2D can be activated under the stimulus of human NKG2D ligand MICA / MICB, so that green fluorescence emitted by the cells can be detected by GFP (green fluorescent protein) expression.