The invention discloses a hot pot seasoning containing an antibacterial peptide; no preservatives are added, spicy fragrance is strong, spicy flavor is soft, no raw spicy flavor exists, especially with scientific compounding of a composite antibacterial peptide, conventional food preservatives are completely replaced, the composite antibacterial peptide not only contains bacteria antibacterial peptides and capsicum antimicrobial peptides, but also has a natural chili pepper fragrance and soft spicy flavor, and is quite suitable for addition of the hot pot seasoning; with scientific compoundingof a seasoning sauce, the taste and flavor of the hot pot are enhanced; the functional properties such as the antioxidant, antibacterial and pharmacological properties of the hot pot seasoning are enhanced, and comprehensive flavor substances of food materials in a roast-smoking state are collected, so the hot pot seasoning has a strong and attractive characteristic roast-smoked flavor, and a leading card of organic integration of the roast-smoked flavor and the hot pot seasoning are created; a hot pot soup prepared by the hot pot seasoning has the advantages of delicious taste, outstanding beef flavor, strong roast-smoked flavor, low cholesterol content and strong food safety, and a new shortcut is explored for preparation of the hot pot seasoning with functional characteristic flavor.