The invention belongs to the technical field of animal model construction, and relates to a construction method of a Parkinson's disease animal model. The construction method includes the step: integrally applying an inhibitor of a vesicular monoamine transporter II and an environmental neurotoxin to an animal. According to the construction method, the inhibitor of the vesicular monoamine transporter II and the environmental neurotoxin are targeted to the VMAT2 (vesicular monoamine transporter II) and a mitochondrial complex I, the PD (Parkinson's disease) animal model induced by non-single factors is coincident with an etiological idea that a Parkinson's disease is induced by multi-factors, and characteristics of human Parkinson's diseases can be more effectively simulated. According to the construction method, DTBZ and MPTP are combined to construct the Parkinson's disease animal model, dosage of the MPTP is remarkably lower than that of the MPTP in a classical Parkinson's disease model induced by the MPTP, on one hand, slow progressing characteristics of the human Parkinson's diseases can be effectively simulated, on the other hand, experiment operators are obviously protected,and side effects are reduced.