The invention relates to a powerful antifatigue super oxide dismutase (SOD) health wine and a preparation method thereof, belonging to the technical field of health food. The health wine is prepared by mixing the following main traditional Chinese medicine raw materials, such as ginseng, cordyceps sinensis, gynostemma pentaphylla, cortex lycii radicis, cartialgenous, astragalus mongholicus, angelica sinensis, safflower, penisotariae, Chinese yam, green plum, medlar, sea otter, sea horse and plant extract-SOD with a proper amount of distilled spirit. The health wine has the effects of powerfulanti-fatigue, improving immunity, generating sperm and tonifying the kidney, stimulating the menstrual flow and invigorating the circulation of blood, keeping up spirits and tonifying qi, invigorating the circulation of blood and eliminating stasis, strengthening tendon and bone, activating physiological functions, promoting metabolism of human body and the like and has the functions of enhancingsexual passion, enlarging penis, lengthening sexual intercourse time, beautifying people and eliminating toxicant, promoting human health and the like and enjoys obvious opsonic action on impotence, premature ejaculation, sexual debility, sexlessness, soreness of waists and pain of legs, numbness of muscles and bones, abnormal menstruation and dysmenorrhea. In the preparation method of the invention, the traditionally medicinal material soaking production technology is replaced by the production technology in which a raw material extract liquor is used for preparing the wine, thus not only effects and nutritious ingredients of the medicine are improved, but also production time is reduced; meanwhile, the health wine prepared by the method is free from animal fishy taste, zoohormone, and strong foreign flavour of medicines and is a natural green health food.