64 results about "Disease pathogenesis" patented technology

The present invention provides methods and systems for conditioning cerebrospinal fluid (CSF). The methods provide for efficiently removing target compounds from CSF. The systems provide for a multilumen flow path and exchange of a majority volume portion of CSF in the CSF space. The removal and/or delivery of specific compounds can be tailored to the pathology of the specific disease. The removal is targeted and specific, for example, through the use of specific size-exclusion thresholds, antibodies against specific toxins, and other chromatographic techniques, as well as delivery and/or removal of targeted therapeutic agents. The invention finds use as a diagnostic, therapeutic and drug delivery platform for a variety of diseases affecting the CNS by accessing the CSF space. Exemplified disease conditions treatable by the present CSF processing systems and methods include, but are not limited to: Cerebral Vasospasm, Guillain Bane Syndrome, illustrating multi-lumen lumbar approach Alzheimer's, Parkinson's, Huntington's, Multiple Sclerosis, Amyotrophic Lateral Sclerosis, Spinal Cord Injury, Traumatic Brain Injury, Stroke, Cancer affecting the brain or spinal cord, Prion disease, Encephalitis from various causes, Meningitis from various causes, diseases secondary to enzymatic or metabolic imbalances, Biological Warfare, etc. For the first time, the present invention offers patients a disease-modifying, disruptive technology treatment platform that addresses the known disease pathogenesis of a number of neurologic conditions to which there are presently limited and ineffective treatment options.

The Chinese herbal medicine preparation for puerpera to promote lactation and strengthen physique is oral liquid or granule prepared with astragalus root, angelica, Chuanxiong rhizome, dipsacus root, vegetable sponge and other 11 kinds of Chinese medicinal materials. The present invention is developed based on Chinese medicine theory and disease treating principle and through long term research. The Chinese herbal medicine preparation can regulate the immunity of puerpera comprehensively, dredge milk secreting gland tissues, strengthen hypophysis function, tranquilize, etc. and has obvious curative effect, high safety and low cost.

In one or more embodiments, the present invention provides a novel biomarker which provides a link between a distinct clinical phenotype and a biochemical effect of an autoantibody on an enzyme implicated in disease pathogenesis. In particular, the present invention provides an isolated or purified human autoantibody to PAD3 protein. Methods of diagnosis of subjects for rheumatoid arthritis (RA) using these antibodies as well as diagnosis of the severity of RA in the subject, and methods for monitoring treatment of a subject with RA are also provided. The biomarkers provided herein are also useful in the diagnosis of connective tissue-interstitial lung disease (CT-ILD) in patients having or suspected of having RA.

The invention discloses a traditional Chinese medicinal composition for treating mammary gland tuberculosis. According to the traditional Chinese medicine, the disease pathogenesis are stagnation of liver qi, deficiency of lung and kidney yin, hyperactivity of fire due to yin deficiency, phlegm fire connotation, pus formation due to flesh decay, leakage after burst, prolonged healing, and deficiency of Qi and blood. According to the invention, traditional Chinese medicines scarlet kadsura root, pedunculate acronychia wood or root, villous amomum flower and peduncle, moniliform dendrobium herb, dunn antiotrema root, laxflower embelia root, Hydrangea aspera Don, wintersweet root or stem and nickernut caesalpinia leaf for coursing the liver, rectifying qi, nourishing yin, reducing inner heat, reducing phlegm, resolving masses, moving qi, invigorating blood, relieving swelling and dissipating binds are prepared into an oral decoction, and Japanese mazus herb, variegatedleaf begonia herb, Chinese squill, circaeashape loosestrife herb, emblic leafflower bark and Japanese spiraea leaf are prepared into an external ointment; the composition conducts internal and external therapy on patients and reaches a total effective rate of 93.75% through clinical test, and shows no side effects.

The invention discloses a method for enriching low-abundance protein in serum. The method adopts heparin-agarose as a filler for a purification column; 1.5 mol/L NaCl is used as an eluant; a composition obtained from elution contains abundantly-enriched low-abundance protein; the content of high-abundance protein is very low; the method solves the problem of the interference of the high-abundance protein in the serum, obtains the low-abundance protein of the target class needed in the research and provides the research condition for the further in-depth study of disease diagnosis of a high-efficiency and special low-abundance protein identifier. The method selects the heparin-agarose as a purification material which has low price, simple and feasible chromatography process and good purification effect, can obtain serum protein with more varieties; and the heparin-agarose can be repeatedly utilized. The method can be used for manufacturing a merchandised albumen purification column and has large significance on applying functional proteomics to the search of a disease diagnosis marker, the research of disease pathogenesis and the development and popularization of medicaments.

The invention discloses a method for calculating and identifying protein kinase phosphorylation specific sites, which is characterized in that, the method comprises the following steps: a) establishing a new protein sequence structure characterization method-amino acid three-dimensional character scoring based on the active ingredient analysis method; b) using the amino acid three-dimensional character scoring for characterizing the structure features of the protein kinase phosphorylation specific sites; c) using a Fisher criterion scoring method for selecting parameters which are closely related to the features of the protein kinase phosphorylation specific sites; and d) and establishing a protein kinase phosphorylation specific site identification model by a radial basis kernel support vector machine, carrying out the self-replacement verification respectively, and using the retaining 1/10 cross-verification and the external verification for verifying the predictive capability of the method. The method can be used for the identification of the protein kinase phosphorylation specific sites, explore the protein phosphorylation rules under physiological and pathological states, further elaborate the nature of life and the disease pathogenesis and provide the important support for developing new drugs.

The present invention relates to means and method for isolating naturally-occurring microorganisms (non-pathogenic bacteria, yeasts or fungi) capable of binding toxins from microorganisms such as bacteria, viruses, fungi, yeasts, or protozoans and/or receptors for these toxins on the surface of mammalian cells, thereby making these receptors inaccessible for said toxins. The naturally-occurring microorganisms that are obtainable by the means and methods of the present invention can be used for adsorbing toxins from pathogenic microorganisms and/or blocking receptors for such toxins on the surface of mammalian cells. These toxin-receptor interactions are known to be critical for disease pathogenesis, making both the toxins and receptors a target for the naturally-occurring microorganisms of the present invention.

Described herein is a microphysiological system for models of disease. Specifically, induced pluripotent stem cells (iPSCs) and iPSC-derived cells, including those obtained from disease patients, are seeded onto microfluidic “chip” devices to study cellular development and disease pathogenesis. Herein, neurodegenerative disease modeling, including Parkinson's Disease (PD) is shown to reproduce key PD pathology in a vascularized human model that contains neurons relating to PD pathology. Such compositions and methods are used for research for PD biomarkers, patient screening for PD risk assessment, and therapeutic discovery and testing. A panel of biomarkers are generated through analysis of living PD-chips by neural activity, whole transcriptomic, proteomic, and metabolomic analysis, and functional enzyme tests of media and tissue. Introducing therapeutics through a vasculature channel, coupled with blood brain barrier penetration studies can be assessed for efficacy in the human neural cells present in the PD-Chip.

The invention is directed to co-culture systems comprising (i) rotating wall vessel (RWV)-cultured epithelial or differentiated tissue attached to microcarrier beads and (ii) the peripheral tissue equivalent (PTE) module of the MIMIC® system, and to methods of using the co-culture systems for assessing chemical or biological (bacterial or viral) insults. The system models mucosal exposure to chemicals, pathogens or antigen at various sites in the human body. The microcarrier and MIMIC® co-culture approach provides an in vitro co-culture model that simultaneously demonstrates mucosa-mediated antigen presentation and immunogenic responses. Models of the present invention can be used, for example, in assessments of disease pathogenesis and in pharmaceutical development, reproductive physiology, and immunological and toxicological evaluations. Models of the present invention can generate patient-specific localized mucosal immunology using primary cells, resembling the human physiological situation.

Induced pluripotent stem cell (iPSC)-based organoid technology has tremendous potential to elucidate the intestinal and colonic epithelium's role in health and disease. Described herein are methods and compositions for generation of intestinal and colonic cells from iPSCs. Derivation of iPSCs from subjected afflicted with early onset and very early onset Inflammatory Bowel Disease (IBD), serves as an excellent model for understanding disease pathogenesis.

This invention relates to statistically significant methods for metabolomics data analysis that incorporate the structure information of metabolites. Understanding of disease pathogenesis and drug effects, as well as prediction of variation in drug response can be achieved by analyzing quantitative data measuring metabolomics biomarker profiles from biological samples. This invention is to boost the statistical power of analyzing metabolomics data. The comprising methods may include retrieving information of metabolites' chemical structures, converting them into structural data, and integrating the structural data into analysis of metabolite concentration data to improve the evaluation of metabolites and to better identify metabolomics signatures.

The invention discloses a making method of human cockayne syndrome specificity adult stem cells. The method comprises the following steps of (1) performing reprogramming on adult fiber cells from cockayne syndrome patient sources carrying cockayne syndrome specificity ERCC6 gene mutation in an excised manner, so as to obtain induction multipotential stem cells namely CS-iPSC; and (2) performing directional induced differentiation on the CS-iPSC to obtain adult stem cells, so as to obtain the human cockayne syndrome specificity adult stem cells. The prepared mesenchymal stem cells or neural stem cells can be used as an effective platform for high-efficacy high-flux individualized drug screening, and a foundation is established for disease research, disease model development, disease pathogenesis research and disease treatment. The making method has great application prospects in individualized treatment and translational medicine.

The invention discloses a construction method for constructing an Alzheimer's disease animal model and a nucleic acid composition and application thereof, and relates to the technical field of genetic engineering. According to the invention, Thy1-hAPP Sweep, Indiana and Thy1-hPSEN1 M146V and L286V are inserted into a target animal genome and are specifically expressed in a brain region, so that an animal model which can be stably inherited and has a spontaneous classic phenotype of Alzheimer's disease (AD) is obtained. Amyloid protein deposition occurs when the animal model is 1.5 months old, and the amyloid protein deposition increases along with age and is age-dependent. Pathologically along with glial cell hyperplasia, the appearance time is earlier than that of a common AD model. Besides, on the basis that the animal model generates a large amount of A beta deposition, the cognitive and memory ability declines when the animal model is 5 months old, and a convenient and effective way is provided for function analysis of AD related genes, research of disease pathogenesis and drug research and development.