Microfluidic chip and design method and application thereof

A microfluidic chip and fluid technology, applied in biochemical equipment and methods, methods for stress-stimulated microbial growth, chemical instruments and methods, etc., to achieve the effects of good biocompatibility, easy operation, and high capture efficiency

Pending Publication Date: 2020-01-03
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the problem of separation and capture of two kinds of single cells in the above-mentioned background technology, and also solves the research problem of the interaction between multiple cells and single cells

Method used

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  • Microfluidic chip and design method and application thereof
  • Microfluidic chip and design method and application thereof
  • Microfluidic chip and design method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Such as figure 1 , the fabrication of the microfluidic chip in this embodiment adopts the soft lithography method, including the following steps:

[0050] Mold production: heat the silicon wafer in the water tiger acid solution to boiling for 30 minutes, wash it with ultrapure water, and blow it dry with nitrogen gas. 65, baked for 10 minutes, 95m for 4 minutes, exposed 3 times with a UV exposure machine, each time for 30s, and dried with nitrogen after development, and the pattern of cell capture was obtained on the silicon wafer.

[0051] Chip production: Add trifluoro(1H, 1H, 2H, 2H perfluorooctyl) silane and mold in a dry vessel, keep the vacuum for 2 hours to make the surface of the mold hydrophobic, polydimethylsiloxane (PDMS) prepolymer Mix with the initiator at a mass ratio of 10:1 and pour into the mold, and place it at 75 for 2 hours after removing air bubbles. The polymerized PDMS sheet is peeled off, cut into shape, punched with a flat syringe needle, and ...

Embodiment 2～4

[0057] The method for designing the size of each part of the microfluidic chip includes the following steps:

[0058] 1) Utilizing the principle of “the least path of flow resistance”, the flow of fluid in the channel usually occurs at a low Reynolds coefficient (Re). For laminar flow in a rectangular channel without drop and obstacles, the resistance calculation formula is

[0059]

[0060] Among them, μ is the viscosity of the fluid, Q is the flow rate, Q=V×A, V is the flow velocity, A is the cross-sectional area of ​​the channel, L, w, h are the length, width and height of the channel respectively, and α is related to the aspect ratio The dimensionless parameter of ,

[0061]

[0062]

[0063] 2) Calculate the pressure drop △P:

[0064] For the resistance of the capture unit, it is approximated as a rectangular resistance;

[0065] The pressure drop of the fluid through the first main channel and the narrow channel, where P 0 Indicates narrow channel, P 1 Indic...

Embodiment 5～8

[0080] Water is used as the simulated material, the length of the main channel between the capture units is 150 μm, the width and length of the entrance of the capture unit remain unchanged, and the shape of the capture unit is changed. The shapes of the capture units in Examples 3 to 8 are triangle, semi-ellipse, square and circle respectively .

[0081] Such as Figure 5 As shown, the four shapes of the capture unit have no effect on the flow resistance, but the fluid flow lines passing through the capture unit are different. When the capture unit is triangular and semi-elliptical, the fluid is more aggregated, which can reduce the accumulation of cells in the capture unit. Cells occupy a larger area ratio. The circle and rectangle have more nonlinear fluid in the capture unit, and the pressure diagram shows that the circle and rectangle are more likely to generate shear force at the connection between the main channel and the capture unit.

[0082] 1. Single-cell capture ...

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Abstract

The invention discloses a microfluidic chip and a design method and application thereof. The microfluidic chip includes a main channel, capture units, and a narrow channel; the main channel is used for transferring cells and cell culture media; the plurality of capture units are arranged on the side wall of the main channel oppositely and at intervals, and the capture units are used for capturingand culturing single cells; the narrow channel is used for communicating two capture units to form an communication path between the single cells after capture and preventing the captured single cellsfrom flowing into the main channel on the opposite side. The microfluidic chip is simple to make and easy to operate, the capture and culture of two kinds of single cells can be realized, researcheson cell-cell interactions are conducted, and the microfluidic chip is suitable for exploring cell heterogeneity and disease pathogenesis. The microfluidic chip can be used for carrying out researcheson the mechanism of tumorigenesis and development and providing a basis and novel research ideas for constructing an effective tumorigenesis model in vitro, and a certain theoretical support is provided for tumorigenesis and development.

Description

technical field [0001] The invention relates to a microfluidic chip and its design method and application, which are used for the capture and culture of single cells in two kinds of cells and the research on the interaction between cells. Background technique [0002] A single cell is the basic unit that constitutes a living body, and traditional cell research is always carried out on a group of cells. The results obtained are often the average value of a group of cells, which conceals the information differences between individuals. In the study of proteomics, it is found that the same cell behaves and responds completely differently in different periods and in different environments. In tumor cells and stem cells Among them, the heterogeneity of cells is the most prominent. Micro-total analysis system, also known as microfluidic chip (microfluidic chip) or lab on a chip (LOC), refers to the integration or basic integration of chemistry and biology on a chip of several squ...

Claims

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Application Information

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IPC IPC(8): C12M3/00B01L3/00
CPCB01L3/502707B01L2200/10B01L2300/0887B01L2300/12C12M23/16C12M35/08
Inventor 林雪霞宿建龙
Owner HUAQIAO UNIVERSITY
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