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Hemi-parkinsonism monkey model and medicament screening method thereof

A Parkinson's disease and model technology, which is applied in the field of monkey hemiparkinson's disease model and its drug screening, can solve the problems of unsafe modeling operation, high MPTP concentration, and low success rate of modeling, achieving economical and safe The effects of increased sex, low drug concentration, and long infusion time

Inactive Publication Date: 2012-03-21
WEST CHINA HOSPITAL SICHUAN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this literature, the injection volume is small, the concentration of MPTP is high, the modeling operation is unsafe, and it is easy to cause the failure of modeling. In the literature, 5 monkeys were used for modeling, and 2 monkeys did not develop PD symptoms and rotation behaviors within 2 weeks. Then repeated injection of MPTP 1 time, the success rate of modeling is low

Method used

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  • Hemi-parkinsonism monkey model and medicament screening method thereof
  • Hemi-parkinsonism monkey model and medicament screening method thereof
  • Hemi-parkinsonism monkey model and medicament screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 The preparation of monkey hemiparkinson's disease model of the present invention

[0037] 1. Purpose of the experiment

[0038] Establish a hemic MPTP monkey Parkinson's disease model with typical symptoms and conduct a comprehensive evaluation of the model to make it suitable for various researches such as new drug evaluation.

[0039] 2. Experimental method

[0040] 2.1 Experimental animals selected:

[0041] Referring to the inclusion criteria of experimental monkeys (see below), 3 healthy adult macaques were purchased from Chengdu Ping An Experimental Animal Breeding Center. Aged 5-7 years old, weight 5.0-6.1Kg, all male, represented by M1, M2, M3 respectively. After passing the inspection and quarantine, they are placed in the Large Animal Breeding Center of the National (Chengdu) Traditional Chinese Medicine Safety Assessment Center (GLP) for breeding.

[0042] Inclusion criteria for experimental monkeys:

[0043] ①, age 4-7 years old;

[0044] ...

Embodiment 2

[0127] Example 2 Validate the model of hemiparkinsonism with drugs for the treatment of hemiparkinsonism and preliminarily establish a related drug screening platform

[0128] A, get the monkey hemiparkinson's disease model prepared in Example 1;

[0129] b. Using currently recognized drugs for the treatment of hemiparkinson's disease (Madopar, Sining) applied to the hemimonkey Parkinson's disease model;

[0130] c. Observe the effect of the candidate drug on the quantitative indicators of hemiparkinson's disease, and score (the scoring standard is the behavioral scoring table in the text), and evaluate the potential drug for treating hemiparkinson's disease. It also verified the feasibility of using the hemimonkey Parkinson's disease model animal of the present invention for drug screening.

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Abstract

The invention provides a preparation method for a hemi-parkinsonism monkey model. The method comprises the following steps of: applying 20 ml of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) of which the dosage is 0.45-0.9 mg / kg to a monkey at the uniform speed 3ml / min. The invention further provides application of an animal model to screening of a medicament for treating the hemi-parkinsonism. The invention further provides a method for screening a medicament for treating the hemi-parkinsonism. The hemi-parkinsonism monkey model established with the method has the advantages of remarkable and typical symptoms and high stability after modeling; and an experimental animal survives easily, and is easy to breed. The stable time of the model is relatively long, and multiple stages of medicament treatment evaluation can be performed. Moreover, the model can undergo own control in the entire disease process, the using quantity of the model is saved, the requirement of the animal experiment ethnics is met, and the model is more economical and reasonable.

Description

technical field [0001] The invention relates to a monkey hemiparkinson's disease model and a drug screening method thereof. Background technique [0002] There are many experimental animal models of Parkinson's disease (PD), including surgical injury of mice and rats or models of damage caused by stereotaxic injection of 6-hydroxydopa. The most valuable model animals should be species that have similar structure, function and complexity to the human nervous system, especially the basal ganglia of the midbrain. The primate PD animal model can reflect the basic characteristics of PD symptoms, and is currently the animal model closest to human PD, which provides a good foundation and platform for the basic research of PD. Liu Sheng, et al., MPTP-induced hemiparkinsonism model in monkeys, "Chinese Medical Imaging Technology", Volume 22, Issue 3, 2006, reported that 1-methyl-4-benzene was injected through one side of the internal carotid artery Base-1,2,3,6-tetrahydropyridine (...

Claims

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Application Information

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IPC IPC(8): A61K49/00A01K67/027
Inventor 李鹏王伟李宏霞王莉刘文科徐策
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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