31results about How to "Reduce the injection dose" patented technology

The present invention is enroloxacin suspension and its preparation process and belongs to veterinary medicine technology. The enroloxacin suspension includes enroloxacin 8-12 wt%, butyl oleate 3-8 wt%, polysorbate 3-8 wt% and propylene glycol 30-50 wt% except water. The enroloxacin suspension is prepared through D phase emulsifying process, and is white and ropy. The enroloxacin suspension has slow release of the effective medicine component, high bioavailability, enhanced medicinal effect, long effective blood medicine concentration maintaining period up to 48 hr, low cost and other advantages.

The invention discloses a siRNA-IR sequence for sex reversal of male macrobrachium rosenbergii and application of the siRNA-IR sequence. Expression of an IR gene is inhibited by a siRNA interference technology to promote sex reversal of the male macrobrachium rosenbergii to obtain neo-females. IR gene fragments of macrobrachium rosenbergii are screened and obtained from macrobrachium rosenbergii transcriptome, and the siRNA is designed and synthesized according to a macrobrachium rosenbergii IR gene sequence, named siRNA-IR. The siRNA-IR is transformed into macrobrachium rosenbergii by a microscopic injection method before a sex differentiation stage to interfere and inhibit expression of the IR gene in the macrobrachium rosenbergii and promote sex interversal of the macrobrachium rosenbergii. Compared with a dsRNA interference technology, the siRNA technology has smaller injection dosage, and can reduce the cost, effectively reduce immunological stress reactions of post-larvae of themacrobrachium rosenbergii and increase the survival rate of neo-females.

The invention discloses a recombinant human chorionic gonadotrophin-Fc fusion protein (HCG-Fc for short) and a preparation method thereof. The HCG-Fc protein is a dimerized fusion protein, and the amino acid sequence of the fusion protein sequentially comprises an HCG beta subunit, an HCG alpha subunit, a flexible peptide joint and a human IgG Fc mutant from an N end to a C end. Compared with the existing chorionic gonadotrophin, the fusion protein has longer in-vivo half life, higher bioavailability and lower side effect. The invention also relates to an application of a recombinant HCG-Fc fusion protein composition in preparation of medicaments for treating and / or preventing infertility.

The invention relates to mixture indicator and an animal indicating method, belongs to an indicating method, and provides mixture indicator and an animal indicating method adopting the mixture indicator. Raw materials of the mixture indicator comprise magnetic particle and edible oil, wherein the magnetic particle at least contains iron and carbon. The mixture indicator is injected into an object to be indicated, a detector determines whether the object is indicated or not, and the indicated object is replaced after the indication is completed. Because the raw materials of the mixture indicator have simple component, stable chemical property, easy obtaining of the raw materials, low cost and easy preparation, the injection quantity of the mixture indicator is little, the indicating speed is very high, and the indicating cost is very low. The invention not only influences the indicated object little, but also has higher safety to a human body, has wide range of application, especially the application to living microbe, such as the cultivation of bionts of rabbit fish, amphioxus, loach, bird, and the like, and can be also used as an indicator of a nonmagnetic product (the product without influencing magnetic lines).

The invention discloses a siRNA-IR sequence of male Macrobrachium rosenbergii sex transition and its application; it uses siRNA interference technology to inhibit the expression of IR gene, promotes the sex transition of male Macrobrachium rosenbergii, and obtains pseudo-female shrimp; The IR gene fragment of Macrobrachium rosenbergii was screened and obtained from the transcriptome of Macrobrachium rosenbergii, and siRNA was designed and synthesized according to the IR gene sequence of Macrobrachium rosenbergii, named siRNA‑IR. Before the sex differentiation period, it was introduced into Macrobrachium rosenbergii by microinjection method, interfered and inhibited the expression of IR gene in Macrobrachium rosenbergii, and promoted the sex transition of Macrobrachium rosenbergii. Compared with dsRNA interference technology, siRNA interference technology has a smaller injection dose, can reduce costs, can effectively reduce the immune stress response of postlarvae of Macrobrachium rosenbergii, and improve the survival rate of pseudo female shrimp.

The invention discloses a medical inflation and curing bone-forming agent for strengthening a vertebral body, which is prepared by mixing the following components in percentage by weight: 10 to 20 percent of expanding agent, 30 to 40 percent of curing agent, 30 to 40 percent of bone-forming agent and the balance of water, wherein the total weight percentage of the expanding agent, the curing agent, the bone-forming agent and the distilled water is 100 percent. The whole strengthening degree of the vertebral body can be obviously enhanced under an osteoporosis condition; the stability of the vertebral body is improved; the leakage of a filled material is reduced; the distribution uniformity of the filled material in the vertebral body is improved; new bones are promoted to be formed in the vertebral body at the same time; an osteoporosis state is improved; and the clinical treatment effect is effectively improved. The medical inflation and curing bone-forming agent is a vertebral body strengthening material, which is obvious in expansion effect and good in mechanical property and bone-forming effect.

The invention particularly relates to a kind of antibody labeled porphyrin iso-indole free radical compound, which has the structural formula as shown in the formula I, and further relates to a synthetic method of the antibody labeled porphyrin iso-indole free radical compound and the application of the antibody labeled porphyrin iso-indole free radical compound used as an electron paramagnetic resonance diagnostic agent. A porphyrin iso-indole free radical compound and a tumor-targeting group GPC3-mAb monoclonal antibody are connected through a covalent bond so as to form a compound; the compound forms the electron paramagnetic resonance diagnostic agent with a medicinal vector or a forming body. The diagnostic agent can be selectively absorbed by hepatoma cells, the targeted imaging can be achieved, the contrast degree and definition of imaging can be improved, the imaging effect is good, the using amount is low, and the toxicity is less.

A dual-targeting polymer magnetic nanoparticle for pancreatic cancer, the magnetic nanoparticle is made of the following components and methods: CKAAKN-hyaluronic acid-vitamin E succinate polymer micelles, ferroferric oxide magnetic nanoparticle solution , deionized water, hyaluronic acid-tocopherol succinate copolymer modified by CKAAKN polypeptide is encapsulated with ultra-small paramagnetic Fe 3 o 4 Made of magnetic nanoparticles, the present invention designs polymer magnetic nanoparticles modified by CKAAKN polypeptides to mimic Wnt protein to specifically bind to pancreatic cancer cell membrane receptors through the Wnt signaling pathway, and at the same time, the surface of tumor cells can also highly express hyaluronic acid Acid receptors——CD44 and hyaluronic acid-mediated automaticity receptors (RHAMM), so they can specifically target pancreatic cancer cells in the early diagnosis of pancreatic cancer, thus providing a specific and imaging method for early diagnosis of pancreatic cancer Dual-targeting MRI contrast agents with enhanced capabilities.

The invention discloses a method for preparing a Gd3<+> magnetic resonance imaging contrast agent with graphene oxide serving as a carrier, and belongs to the technical field of preparation of medical materials. The method comprises the following steps of: 1) dispersing graphene oxide into distilled water to obtain a graphene oxide dispersing solution; 2) adding an alkaline substance and chloroacetic acid or sodium monochloroacetate to the graphene oxide dispersing solution, ultrasonically dispersing, centrifugally washing and processing, and dialyzing to obtain carboxylation graphene oxide; and 3) dispersing the carboxylation graphene oxide into the distilled water to obtain a carboxylation graphene oxide dispersing solution, adding an aqueous solution of gadolinium salt to the carboxylation graphene oxide dispersing solution, adding a DTPA (Diethylene Triamine Pentacetic Acid) aqueous solution after the stirring reaction is done, uniformly mixing to obtain a mixing solution, and dialyzing to obtain carboxylation graphene oxide with Gd3<+> loaded on the surface, thus obtaining the Gd3<+> magnetic resonance imaging contrast agent. The method is simple and feasible, and the Gd3<+> is firmly loaded; and the preparation magnetic resonance imaging contrast agent is high in water-borne dispersion stability and has high longitudinal relaxation rate.

The present invention is enroloxacin suspension and its preparation process and belongs to veterinary medicine technology. The enroloxacin suspension includes enroloxacin 8-12 wt%, butyl oleate 3-8 wt%, polysorbate 3-8 wt% and propylene glycol 30-50 wt% except water. The enroloxacin suspension is prepared through D phase emulsifying process, and is white and ropy. The enroloxacin suspension has slow release of the effective medicine component, high bioavailability, enhanced medicinal effect, long effective blood medicine concentration maintaining period up to 48 hr, low cost and other advantages.

The invention provides a nuclear magnetism-optical dual-mode imaging biconical DNA nanoprobe, and a preparation method and an application thereof. The dual-mode imaging DNA probe is prepared from a DNA nanostructure, a nucleic acid aptamer, a chemical fluorescent molecule and a nuclear magnetism resonance imaging contrast agent. The DNA probe provided by the invention improves the stability of the nuclear magnetism resonance imaging contrast agent and the chemical fluorescent molecule in body circulation, reduces the potential biological toxicity of gadolinium ions, and improves the targeting ability of tumors by using the nucleic acid adapter, and maintains the T1 relaxation efficiency equivalent to that of a clinical nuclear magnetism resonance contrast agent when the gadolinium content is only 1 / 20 of the clinical dose. With the synergistic effect of magnetic resonance imaging and fluorescence imaging, the DNA probe achieves higher resolution ratio and sensitivity of tumor imaging, and provides powerful technical means for early diagnosis of tumors.

The invention relates to an injectable composition containing tilmicosin and sulfonamides, and a preparation process thereof. The composition comprises 4.0%-30.0% (W / V) of sulfonamides, 4.0%-20.0% (W / V) of tilmicosin, 0.05%-0.40% (W / V) of antioxidant, 0.01-15% (W / V) of cosolvent, 1.0%-10.0% (W / V) of pH value buffer salt, 0.01%-0.5% (W / V) of preservative and 0.01%-0.04% (W / V) of complexing agent. The injectable composition containing tilmicosin and sulfonamides provided by the invention has the advantages of high drug ingredient content, low usage volume, the alleviation of pain and the reduction of irritation of animals, the reduction of adverse effect and safety accidents caused by an organic solvent, low manufacture cost, simple production and the like. The injectable composition containing tilmicosin and sulfonamides can be used for preventing and treating bacterial mixed infection diseases.

The invention discloses a corpse conserving agent and a preparation method thereof. The corpse conserving agent comprises the following raw materials with the following volume content: 150 to 400 parts of formaldehyde, 60 to 200 parts of benzoic acid-ethyl alcohol solution, 20 to 100 parts of sodium acetate solution, 10 to 120 parts of acetone, 3 to 80 parts of glacial acetic acid and 0.5 to 1.5 parts of perfume. The benzoic acid-ethyl alcohol solution is formed by benzoic acid dissolved in 95 percent ethyl alcohol, and the sodium acetate solution is formed by sodium acetate dissolved in distilled water. The preparation method comprises the following steps: (1) taking the benzoic acid to dissolve in the 95 percent ethyl alcohol so as to form the benzoic acid-ethyl alcohol solution and then shaking evenly; (2) taking the sodium acetate solution to dissolve in the distilled water so as to form the sodium acetate solution and then mixing uniformly; and (3) mixing and shaking the formaldehyde, the acetone, the benzoic acid-ethyl alcohol solution, the sodium acetate solution and the glacial acetic acid, and finally dropping the perfume. By using the corpse conserving agent of the invention, only individual positions of the corpse are required to be injected, without corpse immersion, therefore, the purposes of short-term corpse corrosion protection and preservation can be achieved,the toxic action of formaldehyde can be reduced, and the corpse conserving agent has the advantages of mold prevention, security and corrosion protection, stable performance and no pollution to the surrounding environment.

The invention discloses a recombinant adenovirus associated virus vector containing an ND4 gene expression cassette. A skeleton of the recombinant adenovirus associated virus vector is pAAV9, and theND4 gene expression cassette contains a human ND4 gene or an optimized human ND4 gene. The invention also discloses a preparation method and application of the recombinant adenovirus associated virusvector. According to the preparation method, pAAV9 is used as a gene vector of ND4, and when ND4 is transduced into eyes of a patient, the expression amount on the retina is higher while the safety isensured, the higher transduction efficiency and efficient expression capability are achieved, and the optic nerve cell infection capability is stronger. When the recombinant adenovirus associated virus vector is prepared into a vitreous cavity injection medicine, the effect of the gene in Leber's gene therapy can be enhanced, the injection dosage can be reduced, and the healing time can be shortened.

The invention relates to mixture indicator and an animal indicating method, belongs to an indicating method, and provides mixture indicator and an animal indicating method adopting the mixture indicator. Raw materials of the mixture indicator comprise magnetic particle and edible oil, wherein the magnetic particle at least contains iron and carbon. The mixture indicator is injected into an objectto be indicated, a detector determines whether the object is indicated or not, and the indicated object is replaced after the indication is completed. Because the raw materials of the mixture indicator have simple component, stable chemical property, easy obtaining of the raw materials, low cost and easy preparation, the injection quantity of the mixture indicator is little, the indicating speed is very high, and the indicating cost is very low. The invention not only influences the indicated object little, but also has higher safety to a human body, has wide range of application, especially the application to living microbe, such as the cultivation of bionts of rabbit fish, amphioxus, loach, bird, and the like, and can be also used as an indicator of a nonmagnetic product (the product without influencing magnetic lines).

The invention relates to a polysaccharide macromolecular paramagnetic metal complex and a synthesis method and an application thereof. Polysaccharide macromolecules are used as carriers, and a ligand compound, the side chains of which contain open-chain or annular polyamino polycarboxylic compounds, acts with paramagnetic metal ions to form the paramagnetic metal complex, wherein the paramagneticmetal ions are positive divalent or trivalent ions of metal elements, the atomic numbers of which are 21-29, 42 and 44 or 57-71. The polysaccharide macromolecular paramagnetic metal complex can be used as a magnetic resonance imaging contrast agent for lymphatic organs, lymphatic vessels, lymphatic systems and cardiovascular systems of people or other mammals.

The invention discloses a pedicle screw medical anchoring agent. The pedicle screw medical anchoring agent is prepared from additives, fillers and water by mixing according to a weight percentage, wherein the pedicle screw medical anchoring agent comprises: by weight, 50 to 75% of the additives, 10 to 20% of the fillers and the balance water. The pedicle screw medical anchoring agent can condense fast, has slight expansion, can reinforce a pedicle screw trajectory, has obvious fixing effects and good biological performances, can obviously improve a reinforcement degree of a pedicle screw trajectory local under the condition of osteoporosis, can improve stability and reliability of an inner spinal instrumentation, and can effectively improve clinic effects.