The invention discloses a fermented traditional Chinese medicine microbial preparation for a parent grass carp and a preparation method of the microbial preparation and relates to a traditional Chinese medicine microbial preparation. The preparation method of the microbial preparation comprises the steps of activating and carrying out enlarged culture on aspergillus oryzae, bacillus subtilis, lactobacillus plantarum and candida utilis, grinding astragalus mongholicus, codonopsis pilosula, bighead atractylodes rhizome, rehmannia glutinosa, Chinese angelica, radix paeoniae alba, pimpinella thellungiana, eucommia, herba epimedii, cuscuta chinensis, a glossy privet fruit, fructus lycii, tangerine peel, rice sprouts and the like to form traditional Chinese medicine powder, preparing soya bean meal, maize cob particles, bran and the like into a solid fermentation medium, putting the solid fermentation medium into a fermentation tank, inoculating aspergillus oryzae seed liquid, candida utilis seed liquid, bacillus subtilis seed liquid and lactobacillus plantarum seed liquid, carrying out aerobic fermentation, and after 36-48h of the fermentation, carrying out anaerobic fermentation to form the fermented traditional Chinese medicine microbial preparation for the parent grass carp. The microbial preparation can be used for antenatal intensive culture and postnatal rapid recovery of the parent grass carp with multiple spawning per year, improves disease resistance of the parent grass carp and increases the egg laying amount.