Pancreatic cancer double-targeted polymer magnetic nanoparticles and preparation method and application thereof

A technology of magnetic nanoparticles and polymers, applied in the field of biomedicine, can solve the problems of weak specificity and imaging ability, poor tumor selectivity of pancreatic cancer, and large injection volume of contrast agent.

Active Publication Date: 2019-03-01
贵州中医药大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0016] 2. The hyaluronic acid-vitamin E succinate polymer micelles in the prior art have not been modified by polypeptides, and have the following defects when used in magnetic resonance imaging: 1) The in

Method used

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  • Pancreatic cancer double-targeted polymer magnetic nanoparticles and preparation method and application thereof
  • Pancreatic cancer double-targeted polymer magnetic nanoparticles and preparation method and application thereof
  • Pancreatic cancer double-targeted polymer magnetic nanoparticles and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0091] Dual-targeting polymeric magnetic nanoparticles for pancreatic cancer

[0092] Ingredients: CKAAKN-hyaluronic acid-vitamin E succinate polymer micelles, iron ferric oxide magnetic nanoparticle solution, deionized water.

[0093] Preparation:

[0094] Weigh CKAAKN-hyaluronic acid-vitamin E succinate polymer micelles and dissolve them in deionized water, add oil-soluble ferric iron tetroxide magnetic nanoparticle solution under ultrasonic stirring, stir for 15-25min under ultrasonic, after 45 Rotary evaporation in a water bath at -65°C to obtain targeted polymer magnetic nanoparticles: CKAAKN-hyaluronic acid-vitamin E succinate@USPIO.

Embodiment 2

[0096] Dual-targeting polymeric magnetic nanoparticles for pancreatic cancer

[0097] Ingredients: 40 mg of CKAAKN-hyaluronic acid-vitamin E succinate polymer micelles, 50 μL of ferroferric oxide magnetic nanoparticle solution, and 16 ml of deionized water.

[0098] Preparation:

[0099] Weigh CKAAKN-hyaluronic acid-vitamin E succinate polymer micelles and dissolve them in deionized water, add oil-soluble ferroferric oxide magnetic nanoparticle solution under ultrasonic stirring, stir for 15 minutes under ultrasonic, and place in a water bath at 45°C Rotary evaporation to obtain targeted polymer magnetic nanoparticles: CKAAKN-hyaluronic acid-vitamin E succinate@USPIO.

Embodiment 3

[0101] Dual-targeting polymeric magnetic nanoparticles for pancreatic cancer

[0102] Ingredients: 60 mg of CKAAKN-hyaluronic acid-vitamin E succinate polymer micelles, 150 μL of ferroferric oxide magnetic nanoparticle solution, and 24 ml of deionized water.

[0103] Preparation:

[0104] Weigh CKAAKN-hyaluronic acid-vitamin E succinate polymer micelles and dissolve them in deionized water, add oil-soluble ferric iron tetroxide magnetic nanoparticle solution under ultrasonic stirring, stir under ultrasonic for 25min, and place in a 65°C water bath Rotary evaporation to obtain targeted polymer magnetic nanoparticles: CKAAKN-hyaluronic acid-vitamin E succinate@USPIO.

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Abstract

The invention discloses pancreatic cancer double-targeted polymer magnetic nanoparticles. The pancreatic cancer double-targeted polymer magnetic nanoparticles are prepared from the following components: CKAAKN-hyaluronan-vitamin E succinate polymer micelle, a ferroferric oxide magnetic nanoparticle solution and deionized water through a following method that a hyaluronan-vitamin E succinate copolymer modified by CKAAKN polypeptides is wrapped with ultra-small paramagnetic Fe3O4 magnetic nanoparticles. According to the pancreatic cancer double-targeted polymer magnetic nanoparticles, the polymer magnetic nanoparticles subjected to targeted modifying by the CKAAKN polypeptides can simulate a Wnt protein to be specifically bound with a pancreatic cancer cell membrane receptor through a Wnt signaling pathway, meanwhile, a hyaluronan receptor CD44 and a receptor for hyaluronan-mediated motility (RHAMM) can also be highly expressed on the surfaces of tumor cells, therefore, pancreatic cancercells can be subjected to specific targeting in early diagnosis of pancreatic cancer, and accordingly, a double-targeted magnetic resonance contrast agent with higher specificity and imaging abilityis provided for early diagnosis of the pancreatic cancer.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a pancreatic cancer double-targeting polymer magnetic nanoparticle and a preparation method and application thereof. Background technique [0002] At present, the diagnosis of pancreatic cancer is mainly through imaging diagnosis and detection of serum tumor markers. Carbohydrate antigen CA19-9 is still the most widely used serum tumor marker for pancreatic cancer diagnosis and recurrence monitoring in clinical practice, and has a high diagnostic value for pancreatic cancer. Highly expressed and thus lack sufficient specificity. The commonly used imaging detection methods for pancreatic cancer include surface ultrasound, magnetic resonance imaging (MRI), computed tomography (CT) and positron emission computed tomography (PET), etc. Often helpless, unable to make an accurate diagnosis. Therefore, in order to improve the diagnostic rate and diagnostic level of early pancreatic cancer,...

Claims

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Application Information

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IPC IPC(8): A61K49/14A61K49/12A61K49/18A61K47/42A61K47/36
CPCA61K49/12A61K49/14A61K49/1863A61K49/1866
Inventor 汪祖华周英姜成菊宋信莉缪艳燕张永萍陈晓兰朱修良
Owner 贵州中医药大学
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