Primates Parkinson's disease model behavior assessment optimal method

Abstract

The invention belongs to the technical field of bioscience, and particularly relates to a primates Parkinson's disease (PD) model behavior assessment optimal method. The method comprises the steps of establishing a rhesus monkey PD animal model; injecting MPTP into muscle of a rhesus monkey once a day till typical and stable PD clinical symptoms appear, wherein dosage is 0.2-0.5 mg / kg according to individual sensitivity difference; conducting behavior assessment on the rhesus monkey at two fixed time periods each day while MPTP injection is conducted, and taking the average score of one week as the final score of the week. A set of complete primates PD model behavior assessment method is provided and can be used for new drug efficacy assessment and horizontal and vertical comparative study of different new drugs. The assessment method absorbs the advantages and discards the disadvantages of different rating scales, and can reflect individual clinical difference comprehensively and accurately based on practical experience and clinical performance characteristics of rhesus monkeys, and individual clinical difference includes individual sensitivity difference, disease severity difference, disease sequence difference and individual PD feature difference.

Description

technical field [0001] The invention belongs to the technical field of life sciences, and in particular relates to a behavioral evaluation and optimization method of a primate Parkinson's disease model. Background technique: [0002] Parkinson's disease (Parkinson's disease, PD) is an senile neurodegenerative disease, the incidence rate is second only to Alzheimer's disease, the main clinical symptoms are tremor, muscle rigidity, slow movement and abnormal posture balance, the main pathological features are Substantia nigra dopamine (dopamine, DA) neurons severe degeneration loss and Lewy body (lewy body, LB). PD mainly occurs in the elderly population over 60 years old, with an incidence rate of 1%-2%, and the incidence rate of people over 40 years old exceeds 0.1%, showing a younger onset trend. With the prolongation of the average life expectancy of the population and the emergence of aging, the incidence of PD is on the rise. It is estimated that by 2030, the number of ...

AI Technical Summary

Problems solved by technology

So far, there are various methods of using primates to make PD models, including models of acute unilateral damage, models of acute bilateral damage (such as intraperitoneal and intramuscular injection of modeling reagents), and models of subacute or chronic systemic damage (such as muscle Injection, intranasal spray modeling reagents), etc., each modeling method has different advantages and disadvantages, and the clinical manifestations of animals also vary greatly

The diagnosis of Parkinson's disease and its models mainly depends on behavioral evaluation, but the current behavioral scoring standards are uneven, with different emphases. There is no comprehensive, comprehensive, and universal scoring standard for the primate PD model

Images