The invention discloses a method for performing
genome amplification by using
embryo blastula-stage cells, performing
chromosome detection on the preimplantational
embryo by combining a high-flux sequencing technique and screening out the
chromosome normal
embryo. The method can comprehensively and completely analyze the
genetic variation information of the embryo
genome, thereby instructing the preimplantational embryo selection, reducing the
hereditary diseases and enhancing the success rate of
test tube babies. The method comprises the following steps:
blastula-stage trophocyte separation;
genome amplification;
DNA (deoxyribonucleic acid) segmentation; and
Proton library establishment, mounting sequencing and
sequencing data analysis. By using the
blastula-stage embryo to perform trophocyte separation detection, the method avoids the injuries of cleavage-stage
cell separation to the embryo, obtains higher
cell quantity than the cleavage stage, and enhances the success rate and amplification effect of
genome amplification. After the blastula-stage embryos are subjected to the natural
elimination process, the high-quality blastula-stage embryo is selected for detection, thereby saving the cost.