Method for single cell classification and screening and device therefor

Abstract

The invention provides a method for single cell classification and screening and a device therefor. The method comprises the following steps of carrying out comparison of reads obtained by sample sequencing and a reference genome, carrying out data filtration of a comparison result, determining a consistent genotype of all single cell samples according to filtered data, saving the consistent genotype of the all single cell samples into a SNP data set, extracting genotype files of loci corresponding to reference genome SNP data set positions from the saved SNP data set, selecting a cell mutation SNP locus, and carrying out cell classification and functional gene screening according to a genotype file of the cell mutation SNP locus. The method and the device avoid cell marking, solve the problem that the traditional single cell classification method can not realize classification of a certain cell subset having no corresponding specific markers, realize complete analysis of genetic variation information of a single cell genome, and greatly improve the accuracy of cell subset classification.

Description

technical field [0001] The present invention relates to bioinformatics, and in particular to single cell sorting and screening methods and devices used in said methods. Background technique [0002] There are significant differences in gene expression, copy number variation, and epigenetics among different individuals, between different tissues of individuals, and even different parts of the same tissue. Cell-to-cell heterogeneity also exists, even in vitro cultured cell populations with identical genetic backgrounds. Cellular heterogeneity is particularly pronounced for stem or precursor cells, since any change in state is heritable. In order to better study cell biology and reveal the rules of cell heterogeneity, it is very necessary to develop technical methods applied to single cell research. Therefore, some scholars proposed the concept of "single cell analysis (SCA)", from "omics (Omics) " point of view. Single cell sorting and screening provide an important basis f...

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Problems solved by technology

On the one hand, these techniques use surfactants, fluorescent dyes, and antigen antibodies, which are highly toxic to cells and can only sort specifically labeled or non-specifically labeled single cell suspensions. The early sample preparation process is cumbersome, and currently many fluorescent The specificity of probes and monoclonal antibodies (including CD molecules on the cell surface) is more controversial, and many cell subpopulations do not have corresponding specific markers / specific antigens; on the other hand, these technologies use biology, immunology, chemistry, etc. Methods, through phenotypic measurement (including cell size, cell granularity, cell surface area, nuclear-to-cytoplasmic ratio, etc.), for statistical analysis, the sensitivity for subgroup classification, screening and detection is low, and there is a lack of effective accuracy assessment

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