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In vitro conservation method of churysanthemum by reducing macroelements in the medium

A large amount of elements, in vitro preservation technology, applied in horticultural methods, plant preservation, botanical equipment and methods, etc., can solve the problem of frequent transfer of normal subculture preservation of tissue culture, susceptibility to extreme natural disasters and pests and diseases, preservation Large workload and other issues to achieve the effect of preventing caste degradation and virus infection, facilitating transportation and communication, and reducing succession work

Inactive Publication Date: 2007-08-01
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] Technical problem The purpose of the present invention is to provide a method for reducing the amount of cultivation and preservation of chrysanthemum germplasm resources due to the large workload of planting and preservation in the field, which are easily affected by extreme natural disasters and pests, and the normal subculture of tissue culture, frequent transfer and high cost. The method of in vitro preservation of chrysanthemums with a large amount of elements in the base is used for in vitro preservation of chrysanthemum germplasm resources. Not only the plants grow normally after one year of preservation, but also the offspring of the preserved materials have no genetic variation

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  • In vitro conservation method of churysanthemum by reducing macroelements in the medium

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Embodiment Construction

[0029] The method for preserving chrysanthemum germplasm resources in vitro provided by the present invention, its embodiment is as follows:

[0030] 1. In vitro preservation of chrysanthemum germplasm resources: 'Olympic Torch' (Dendranthema×grandiflorum Ramat.'Aoyunhuoju') selected by our research group (known and public varieties, see references Chen Fadi, Fang Weimin, etc. New varieties of chrysanthemums Yixia flower type potted Chrysanthemum series. Acta Horticultural Science, 2005, 32(3): 567) varieties were used as test materials. The foot buds of 'Olympic Torch' were taken as explants, sterilized with 75% ethanol for 30 seconds, rinsed twice with sterile water, sterilized with 0.1% mercury chloride for 8 minutes, washed five times with sterile water, and then cut into Stem segments with 1 node in the leaves were inoculated into MS medium. After 5 days of explant inoculation, the germination rate of axillary buds was 83.3%; 20 days later, the axillary buds were transfe...

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Abstract

The invention relates to a method for conserving chrysanthemum in vitro through reducing major element in the medium, which is special for the in vitro conservation of the chrysanthemum germplasm resource. The invention comprises the steps of: taking the suckers of the chrysanthemum as the explants, and axillary buds for subculture, and conserving on the 1 / 2MS, 1 / 2MS (1 / 2NH4+) or 1 / 4MS medium containing 6-BA0.3mg / L and NAA0.1mg / L. The survival rate for a 12-month conservation is 100%, 8 months longer than that of conservation on the normal MS medium. After the conservation material recovers to normal growth, the descendant is proved to have no genetic variance through the detection of POD, EST and ISSR. The invention uses the method of conserving chrysanthemum test-tube plantlet through reducing major element in the medium and identifies the genetic stability of the descendant, which proves that the plants grow normally after one-year conservation and the descendant has no genetic variation.

Description

1. Technical field [0001] The invention relates to a method for in vitro preservation of chrysanthemums by reducing a large amount of elements in a culture medium, and is specially used for in vitro preservation of chrysanthemum germplasm resources. 2. Technical background [0002] Chrysanthemum (Dendranthema × grandiflorum Ramat.) is one of the top ten traditional famous flowers in my country and one of the four major cut flowers in the world. It has multiple values ​​such as ornamental, edible and medicinal. my country is the origin center of cultivated chrysanthemums and the distribution center of Chrysanthemum germplasm resources. There are more than 20 species of more than 40 species of Chrysanthemum distributed in my country, and more than 3,000 cultivated chrysanthemum varieties. Cultivated chrysanthemums have a high degree of heterozygosity in genetics, and the traits of offspring are separated. It is difficult to preserve germplasm resources through seeds, but asexu...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00A01H4/00
Inventor 房伟民王艳芳陈发棣管志勇刘兆磊滕年军陈素梅赵宏波许高娟缪恒彬姜贝贝丁玲
Owner NANJING AGRICULTURAL UNIVERSITY
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