The invention discloses an operation workshop scheduling modeling method based on a genetic algorithm. The method comprises the steps of JSP genetic algorithm design of reverse cross of a stored gene segment, eM-Plant simulation modeling, data collection, improvement of mutation operator and obtaining of an optimized scheme; the JSP genetic algorithm design of the reverse cross of the stored gene segment comprises the steps of randomly generating an initial group according to a sequence code, calculating the fitness of the initial group, judging whether the cycling times is satisfied, outputting an optimal result and program running time if the cycling times is satisfied, drawing an algorithm performance trace diagram, drawing an optimal scheduling trace diagram, selecting through a roulette wheel if the cycling times cannot be satisfied, reversely crossing the stored gene segment, randomly mutating the gene segment, calculating the fitness of a novel population, re-inserting a filial-generation population to the parental population, and recording the performance of the optimal result trace algorithm. By adopting the method, the running of the production workshop can be optimized and coordinated, the design effect is good, the process is simple, and the production danger and production cost can be reduced.