Method for predicting chemicotherapy sensitivity by using cervical carcinoma side population cell

Abstract

A disclosed method for predicting chemicotherapy sensitivity by using cervical carcinoma side population cell comprises the following steps: preparing cervical carcinoma cell, and performing in-vitro adherent culture on the cervical carcinoma cell; collecting cervical carcinoma cell at logarithmic phase, and adding the cell into Hoechst33342; sorting SP cells by using a superspeed flow sorting system, and applying SP cell and NSP cell obtained through sorting to further experiment research; and then adding cis-platinum with different concentrations respectively into NSP cell and SP cell, staining by using an Annexin V FITC cell apoptosis detection kit, performing detection by using a flow cytometer, performing different doses of radiotherapy on SP cell and NSP cell, staining by using an Annexin V FITC cell apoptosis detection kit, and performing detection by using a flow cytometer. The cell apoptosis rate is equal to UR% plus LR%. Cervical cancer stem cell screening is performed by taking SP cell as an entry point, chemoradiotherapy sensibility difference and possible mechanism are analyzed, and theoretical basis is provided for researching and developing tumor stem cell resisting medicines, some cytokines interdicting signal path and chemical substances.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for predicting sensitivity to radiotherapy and chemotherapy by using cervical cancer side population cells. Background technique [0002] Cervical cancer is the second most common malignant tumor in women worldwide, second only to breast cancer. There are about 100,000 new cases of cervical cancer in my country every year, accounting for about 1 / 5 of the new cases of cervical cancer in the world. In developing countries, the death rate of women's cervical cancer exceeds that of breast cancer, ranking first among the causes of death from malignant tumors in women. In recent years, the morbidity and mortality of cervical cancer in some areas of our country have been increasing, and some areas have also shown a trend of younger patients. The treatment of cervical cancer adopts a comprehensive treatment plan based on surgery and radiotherapy, supplemented by chemotherapy....

AI Technical Summary

Problems solved by technology

Radiotherapy has been used in various clinical stages of cervical cancer, but its curative effect has not been improved in recent years. Some tumor cells are not very sensitive to radiotherapy. The 5-year survival rate of clinical stage I and II patients after radiotherapy is 65% to 85%. ; About 20% to 50% of patients with stage Ⅲ and Ⅳ

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