Flow sorting device for cell beads

Abstract

The invention provides a flow sorting device for cell beads, and particularly provides a magnetic field generation device. The magnetic field generation device comprises a magnetic generation unit, a fixing device and magnetic noise eliminating assemblies, wherein the fixing device is used for fixing the magnetic generation unit; the magnetic noise eliminating assemblies are arranged at two ends of the fixing device; the magnetic generation unit comprises four magnetic assemblies which are arranged along the same axis; the four magnetic assemblies are fixed to be opposite each other two by two by the fixing device; the four magnetic assemblies surround to form a channel through which a pipeline is permitted to pass; in the magnetic assemblies, the magnetic poles of the opposite surfaces of the two opposite magnetic assemblies are N poles; and the magnetic poles of the opposite surfaces of the other two opposite magnetic assemblies are S poles. The invention further provides a method for sorting cells or separating target molecules on the basis of the magnetic field generation device. By the device and the method provided by the invention, efficient, reliable and high-flux cell sorting and enriching and target molecules separating can be achieved.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a cell magnetic bead flow sorting device. Background technique [0002] Magnetic bead enrichment or separation is an emerging molecular and cell biology technology at the end of the last century, see US Patents US5411863 and US5385707 for details. This technology has been widely used in molecular biology nucleic acid extraction, solid-phase cDNA library construction, protein or cell sorting or enrichment. Its construction principle is to bind specific ligands or antibodies on the surface of microbeads by means of biochemical technology. Its working principle is to mix the magnetic bead suspension with ligand (antibody) on the surface and the target molecule or cell solution (suspension) to be selected, and through the ligand-receptor binding reaction or antigen-antibody reaction, the magnetic beads and the target Molecules or cells are tightly combined, and then the ma...

AI Technical Summary

Problems solved by technology

The magnetic field is only used to capture the sample bound to the magnetic beads, and cannot make any guarantee for the capture efficiency

The magnetic field and its generating device for cell magnetic bead sorting mentioned above cannot effectively guarantee the speed and efficiency of cell sorting due to the design limitation of the magnetic field itself.

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