145results about How to "Low heterogeneity" patented technology

The present inventors succeeded in improving the antibody constant region to have increased stability under acid conditions, reduced heterogeneity originated from disulfide bonds in the hinge region, reduced heterogeneity originated from the H chain C terminus, and increased stability at high concentrations as well as in discovering novel constant region sequences having reduced Fcγ receptor-binding, while minimizing the generation of novel T-cell epitope peptides. As a result, the present inventors successfully discovered antibody constant regions with improved physicochemical properties (stability and homogeneity), immunogenicity, safety, and pharmacokinetics.

The invention relates to methods of increasing the titre of a protein of interest in a cell as well as the improved production and purification of optimised biomolecules, one component of which is the domain CH3. A frequently observed effect in biomolecules is the cleaving of the C-terminal amino acid(s), e.g. the C-terminal lysine. The usually incomplete processing of the heavy chain of antibodies for example leads to product heterogeneity. To prevent this product heterogeneity the corresponding codon of the C-terminal lysine of the heavy antibody chain has been deleted by recombinant DNA technology. These optimised antibodies lead to a product titre which is higher than in the wild-type. In addition, they prove advantageous during purification by having better elution characteristics as a result of the reduced charge heterogeneity.

The invention provides a big data cross-modal retrieval method based on deep integration Hash. The method comprises the following steps that: identifying the data type of data to be retrieved, if thedata type of the data to be retrieved is an image, inputting an image to be retrieved into a trained image Hash network, and obtaining an image binary system code corresponding to the image to be retrieved; calculating a first Hamming distance between the image binary system code corresponding to the image to be retrieved and the binary system code corresponding to each statement in a search library; and selecting a preset quantity of statements with a minimum Hamming distance from the search library as the data type of the data to be retrieved as an image retrieval result. By use of the big data cross-modal retrieval method and system based on the deep integration Hash, an internal cross corresponding relationship between visual data and natural language is captured, so that the compact Hash code of the image and the statement is generated in an end-to-end deep learning framework, and cross-modal retrieval accuracy is improved.

The present invention relates generally to the field of generating fusion proteins to be used in cancer therapy, and more specifically, to nucleotide sequences encoding the fusion proteins, wherein the chimeric fusion proteins comprises at least one targeting moiety and at least one immunomodulatory moiety that counteracts the immune tolerance of cancer cells.

A light emitting device has a light emitting element mounted on a substrate, a glass member sealing the light emitting element, a transparent member to transmit light emitted from the light emitting device, the transparent member being positioned outside the glass member, and a powdery phosphor attached to an inner surface, an outer surface or both of the inner surface and outer surface of the transparent member.

A method for identifying a quantitative trait loci for a complex trait that is exhibited by a plurality of organisms in a population. The population is divided into a plurality of sub-populations using a classification scheme. Depending on what is known about the population, either a supervised or unsupervised classification is used. The classification scheme is derived from a plurality of cellular constituent measurements obtained from each organism in the population. For each sub-population in the plurality of sub-populations, a quantitative genetic analysis is performed on the sub-population in order to identify one or more quantitative trait loci for the complex trait.

Characterizing a reservoir with electromagnetic imaging surveys includes normalizing measured voltage data by transmitter moment, sorting the normalized voltage data into common receiver profiles, densely resampling transmitter locations using common positions for the receiver profiles, coarsely resampling the data at discreet transmitter locations, defining a starting model for inversion, weighting the data by a factor, converting the normalized voltage data to ratios, calculating a conductivity image using a ratio inversion method, and verifying that an inversion has converged and the image is geologically reasonable. The image can then be displayed. The invention can be used for cross-well, surface-to-borehole, and borehole-to-surface measurements by which the effects of steel casing are reduced.

A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

The present invention provides an image correction system and method for multi-projection, the image correction system including a plurality of projection surfaces which are installed in a single theater and a projection device which projects images on the plurality of projection surfaces, in which the images projected by the projection device on the plurality of projection surfaces are corrected based on relative differences in properties of the plurality of projection surfaces.

Commercially pure titanium having UFG structure and enhanced mechanical and biomedical characteristics has nanocrystalline alpha-phase grains with a hexagonal close-packed lattice, in which the share of grains with a size of 0.1 . . . 0.5 μm and a grain shape coefficient of no more than 2 in the mutually perpendicular planes makes no less than 90%, over 60% of the grains having high-angle boundaries disoriented in relation to the adjacent grains by the angles from 15 to 90°.The method for making a rod of the material provides for equal-channel angular pressing of a billet at T≦450° C. with the total accumulated true strain e≧4 to effect severe plastic deformation of the billet and subsequent thermomechanical treatment with a gradual decrease of the temperature in the range of 450 . . . 350° C. and the strain rate of 10−2 . . . 10−4 s−1 with the strain degree from 40 to 80% to effect additional plastic deformation.

Commercially pure titanium having UFG structure and enhanced mechanical and biomedical characteristics has nanocrystalline alpha-phase grains with a hexagonal close-packed lattice, in which the share of grains with a size of 0.1 . . . 0.5 μm and a grain shape coefficient of no more than 2 in the mutually perpendicular planes makes no less than 90%, over 60% of the grains having high-angle boundaries disoriented in relation to the adjacent grains by the angles from 15 to 90°.The method for making a rod of the material provides for equal-channel angular pressing of a billet at T≦450° C. with the total accumulated true strain e≧4 to effect severe plastic deformation of the billet and subsequent thermomechanical treatment with a gradual decrease of the temperature in the range of 450 . . . 350° C. and the strain rate of 10−2 . . . 10−4 s−1 with the strain degree from 40 to 80% to effect additional plastic deformation.

The present inventors succeeded in improving the antibody constant region to have increased stability under acid conditions, reduced heterogeneity originated from disulfide bonds in the hinge region, reduced heterogeneity originated from the H chain C terminus, and increased stability at high concentrations as well as in discovering novel constant region sequences having reduced Fcγ receptor-binding, while minimizing the generation of novel T-cell epitope peptides. As a result, the present inventors successfully discovered antibody constant regions with improved physicochemical properties (stability and homogeneity), immunogenicity, safety, and pharmacokinetics.

The present invention relates generally to the field of generating fusion proteins to be used in cancer therapy, and more specifically, to nucleotide sequences encoding the fusion proteins, wherein the chimeric fusion proteins comprises at least one targeting moiety and at least one immunomodulatory moiety that counteracts the immune tolerance of cancer cells.

The invention provides a cross-modal pedestrian re-identification method and system based on a heterogeneous hierarchical attention mechanism, and the method comprises the steps: extracting pedestrianimage features and text description features, and enabling the pedestrian image features and the text description features to serve as initial global features of a pedestrian image channel and a textdescription channel; Establishing a heterogeneous hierarchical attention model, and enhancing pedestrian picture features and text description features by using a bidirectional cross-mode fine-grained matching attention mechanism and a context-guided local feature aggregation attention mechanism by the model; A heterogeneous hierarchical attention model is trained in a two-stage training mode, preliminary training is carried out in the first stage by utilizing pedestrian category supervision information, training in the second stage is carried out by utilizing cross-modal samples to match thepedestrian category supervision information on the basis, and pedestrian re-identification is carried out by utilizing the trained model. The pedestrian re-identification method and device can improve the accuracy of pedestrian re-identification.

A method for identifying a quantitative trait loci for a complex trait that is exhibited by a plurality of organisms in a population. The population is divided into a plurality of sub-populations using a classification scheme. Depending on what is known about the population, either a supervised or unsupervised classification is used. The classification scheme is derived from a plurality of cellular constituent measurements obtained from each organism in the population. For each sub-population in the plurality of sub-populations, a quantitative genetic analysis is performed on the sub-population in order to identify one or more quantitative trait loci for the complex trait.

The invention relates to a profile modifying / water plugging agent for an oil well, in particular to grafted polymer microspheres for oil displacement modification and a preparation method thereof. The grafted polymer microspheres are prepared from etherified starch, acrylamide, N,N-methylene bisacrylamide and ammonium persulfate in the mass ratio of (1-2) to (0.5-1.5) to (0.04-0.2) to (0.1-0.5). The polymer microspheres have the advantages of controllable particle size, high homogeneity, high temperature resistance, high salt resistance, wide raw material source and low manufacturing cost, is not hydrolyzed in a severe saline environment, and is non-degradable; starch microspheres can further go deep into an oil reservoir after being swelled by absorbing water; meanwhile, the polymer microspheres have the rigid characteristic of starch micro molecules and the flexible changeability characteristic of the acrylamide, have high deepening and blocking performance, and are environmentally friendly; by adopting the grafted polymer microspheres, the non-homogeneity of a rock stratum is lowered, and the crude oil recovery factor is increased.

The present disclosure relates to a method of reducing heterogeneity in antibodies during culturing, wherein the heterogeneity is due to proportion of charge variant of the antibody. The disclosure also comprises a process of growing cells in a cell culture system that results in antibodies with the reduced heterogeneity. In one embodiment antibody heterogeneity is reduced by the addition of divalent transitional metal ions such as zinc (Zn2+) to the cell culture medium. In another embodiment antibody heterogeneity is reduced by decreasing the osmolality of the cell culture medium.

Travoprost medicament compositions for non-daily topical application are useful for simulating or inducing the growth and / or decreasing the loss and / or increasing the density and / or reducing the heterogeneity in the diameter of human hair shafts / follicles, e.g., for the treatment of androgenic alopecia, such medicament compositions being at least twice applied over a time interval of greater than 24 hours.

The invention relates to a spot-color ink color matching method based on particle swarm optimization (PSO). According to the traditional method, spot-color ink color matching can not be precisely realized. The method disclosed by the invention comprises the following steps: calibrating and coloring; solving a correction factor with a polynomial fitting method; then, initializing a PSO algorithm parameter; randomly generating an initial group in a feasible solution space; calculating the fitness value of each individual; revising the position of a particle individual; and finally, detecting a termination condition. With the method, the color matching precision is more accurate, and the color matching efficiency is greatly improved. Spectral distributions between a color matching sample and a standard sample are very close, and metamerism isomerism is explained to be low.

The present invention provides a fusion polypeptide comprising a first domain and a second domain, wherein the first domain comprises a polypeptide ligand which binds to a cell surface-associated antigen and the second domain comprises aglycosylated interferon α 2b (IFNα2b) having a sequence of SEQ ID NO: 1 or SEQ ID NO: 2. The aglycosylated IFNα2b further comprises one or more amino acid substitutions or deletions which attenuate the activity of the aglycosylated IFNα2b.

The present invention discloses a method for producing kapok scribbled by ring spinning, in which the mixed raw materials of kapok fibers and other fibers are subjected to the processes of opening and scutching, carding, drawing, roving, spinning, etc., and a device of guiding cotton sliver is provided after a carding procedure, wherein the said spinning adopts a double-short-apron drafting mode, the said carding is performed twice, and the said drawing is performed twice. The blended yarns produced according to the inventive method have the superior technical indexes in terms of the single yarn breaking tenacity, single yarn elongation at break, etc. which are better than the industrial standard of cotton berber yarn. And the shell fabric produced from the product of the invention has the superior technical indexes in terms of the fabric heat retention, fabric moisture vapour transmission, fabric touching cold-warm feeling, fabric bulking intensity, fabric compressive elastic recovery ratio and fabric soft draping property, etc. which are better than those of the similar products such as the pure cotton and polyester cotton, etc.

By altering amino acid sequences, the present inventors successfully produced constant regions that can confer antibodies with particularly favorable properties for pharmaceutical agents. When used to produce antibodies, the altered constant regions produced according to the present invention significantly reduce heterogeneity. Specifically, the antibody homogeneity can be achieved by using antibody heavy chain and light chain constant regions introduced with alterations provided by the present invention. More specifically, the alterations can prevent the loss of homogeneity of antibody molecules due to disulfide bond differences in the heavy chain. Furthermore, in a preferred embodiment, the present invention can improve antibody pharmacokinetics as well as prevent the loss of homogeneity due to C-terminal deletion in antibody constant region.

Cosmetic / pharmaceutical compositions for promoting hair growth and / or retarding hair loss, and / or for increasing the mean diameter of strands of hair and / or decreasing the heterogeneity thereof, and / or for increasing hair density, and / or for improving the quality and / or the appearance of a head of hair, and / or for inducing repigmentation of the hair, contain thus effective amounts of intimate admixture of vitamin A, vitamin C, vitamin E, and zinc and selenium values.

A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

A spread illuminating apparatus is provided that includes two light conductive plates. Each light conductive plate has point light sources having respective different emission wavelengths and disposed at one side surface thereof. Since a light exiting mechanism is formed at a region in one major surface of the light conductive plate located apart from the light sources, lights emitted from the light sources into the light conductive plate are adapted to exit out mostly from a region in the other major surface located apart from the light sources. The lights are allowed to be well mixed with one another before exiting out the light conductive plate, thus preventing the color heterogeneity and coloration problem. When the two light conductive plates are stacked together such that their respective one side surfaces are oppositely located apart from each other, white light is achieved entirely across the light conductive plate.

The invention discloses a foam producing agent for wet-type dust collection. According to the weight percentage, the foam producing agent for the wet-type dust collection comprises: 0.2%-6% of lauramide propyl glycine betaine, 0.15%-0.3% of hexadecyl tri-methyl ammonium bromide, 0.4%-1.3% of fatty alcohol-polyoxyethylene ether sodium sulfate, 1%-2% of sodium dodecyl benzene sulfonate, 0.2%-0.8% of dodecyl benzene glycine betaine, 0.2%-0.9% of fatty acid glycerolipid, 0.4%-2% of potassium polyacrylate, 1.5%-2.4% of perfluor octyl sulfo tetraethyl ammonium salt, 0.5%-1.5% of sucrose fatty acid ester, 0.3%-1.2% of diocytyl sulfo sodium succinate, and the balance is water. The foam producing agent for the wet-type dust collection is capable of cohering the dusts on the ground for a long time, avoiding the dusts from flying along with the wind because of the drying ground, and performing the collecting function to a duster for a long time. The foams produced by the foam producing agent for the wet-type dust collection have good biological degradability, and both the environmental protection coefficient and the safety coefficient are high.

An optical information medium comprises a supporting substrate, an information recording surface 4 on the supporting substrate and a light-transmitting layer of resin on the information recording surface. Recording light and / or reproducing light are incident on the information recording surface through the light-transmitting layer. The light-transmitting layer is obtained by curing a resin layer containing an ultraviolet-curing cationic resin. If the light-transmitting layer is formed by repeating the cycle of resin coating and curing or drying a plurality of times, the uniformity of thickness of the light-transmitting layer can be much more improved and the amount of warpage can be much more reduced. Repetition of the cycle of resin coating and curing or drying is also effective for resins other than the ultraviolet-curing cationic resin.