36 results about "UDPglucose pyrophosphorylase" patented technology

The invention concerns the use of duplex oligonucleotides about 25 to 30 base pairs to introduce site specific genetic alterations in plant cells. The oligonucleotides can be delivered by mechanical (biolistic) systems or by electrpoporation of plant protoplasts. Thereafter plants having the genetic alteration can be generated from the altered cells. In specific embodiments the invention concerns alteration in the gene that encode acid invertase, UDP-glucose pyrophosphorylase, polyphenol oxidase, O-methyl transferase, cinnamyl alcohol dehydrogenase, ACC synthase and ACC oxidase or etr-1 or a homolog of etr-1, and plants having isolated point mutations in such genes.

The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

The invention discloses a corn endosperm ADP-glucose pyrophosphorylase mutant, encoding genes thereof and application of the same two. The protein can be the protein (1) formed by the first amino acid sequences showed as in a sequence table and can also be the protein (2) which is formed by substituting and / or deleting the first amino acid residue sequences showed as in a sequence table and / or adding one or several amino acid residues to the first amino acid residue sequences, has ADP-glucose pyrophosphorylase activity and is derived from the protein (1). The corn endosperm ADP-glucose pyrophosphorylase mutant and the encoding thereof play important roles in the corn variety improvement.

The present invention relates to novel mutant polynucleotide molecules encoding enzymes with enhanced thermostability. When expressed in plants, these polynucleotides lead to increased yield in plants grown under conditions of heat stress. In one embodiment, the polynucleotide molecule of the present invention encodes maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Also contemplated by the present invention are plants and plant tissues grown to contain or transformed with such mutant polynucleotides and expressing a polypeptide encoded by such polynucleotides. The invention also relates to methods for isolating polynucleotides and polypeptides within the scope of the invention. The present invention also provides methods of increasing yield in plants grown under conditions of heat stress.

The invention relates to an engineering bacterium with overexpressed uridine diphosphoglucose pyrophosphorylase gene and establishment thereof, belonging to the technical fields of gene engineering and microbial fermentation. By cloning the key enzyme uridine diphosphoglucose pyrophosphorylase gene in the polysaccharide flocculant synthesis route, an Escherichia coli-Bacillus shuttle plasmid is utilized to establish a recombinant expression vector. The recombinant plasmid is transformed into Bacillus licheniformis by electric transformation to establish recombinant Bacillus licheniformis HN301-2; and compared with the initial strain, the polysaccharide flocculant yield of the recombinant Bacillus licheniformis is enhanced by 15.6%, and the fermentation liquid flocculation activity is enhanced by 70%. The engineering bacterium is hopeful to be used in industrial production of polysaccharide flocculants, and can enhance the yield and lower the cost.

The invention provides for sequencing a nucleic acid molecule based on the detection of base incorporation by the release of pyrophosphate (PPi) using a new enzyme system comprising adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) and its substrate ADP-glucose.

The invention provides for sequencing a nucleic acid molecule based on the detection of base incorporation by the release of pyrophosphate (PPi) using a new enzyme system comprising adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) and its substrate ADP-glucose.

The subject invention concerns polynucleotides encoding a small subunit of plant AGP having one or more mutations in the amino acid sequence wherein the mutation confers increased heat stability to the expressed AGP enzyme. Mutations in the N-terminus of the small subunit of heat labile plant AGP results in AGP enzymes that are significantly more heat stable compared to wild type AGP in that the mutant AGP retains significant levels of enzymatic activity following exposure to heat treatment. In one embodiment, the polynucleotide encodes a mutant small subunit of maize AGP. The subject invention also concerns methods for providing a plant with increased resistance to heat conditions. Plants with heat labile AGP can be transformed with a polynucleotide of the present invention. The subject invention also concerns these transformed plants and transgenic progeny thereof. The subject invention also concerns mutant polypeptides encoded by polynucleotides of the present invention.

The invention belongs to the technical field of edible fungi hereditary and genetic engineering, and particularly relates to grifola frondosa UDP-glucose pyrophosphorylase and application thereof. Through the genetic engineering technology, the UDP-glucose pyrophosphorylase gene is overexpressed or silenced in grifola frondosa, the quantity of mycelia in grifola frondosa recombinant bacteria and the yield of mycelia polysaccharides and exopolysaccharides can be significantly increased or reduced separately, and the composition of monosaccharide in the mycelia polysaccharides and exopolysaccharides of the grifola frondosa is significantly affected. The grifola frondosa UDP-glucose pyrophosphorylase provides a research basis for mastering a controlling mechanism for synthesis / metabolism of grifola frondosa polysaccharide and efficiently fermenting and producing a stable-quality polysaccharide product.

The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions and / or increased starch biosynthesis. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses as compared to the yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGPase. The subject invention also comprises a mutant plant small subunit of AGPase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.

The invention provides an ADP-glucose pyrophosphorylase mutant and a screening method and application thereof. The screening method includes the steps of firstly, synthesizing first chain cDNA; secondly, performing PCR amplification; thirdly, building AGPase large and small subunit cDNA prokaryotic expression vectors; fourthly, performing active screening. The method has the advantages that distant hybridization is performed on AGPase large subunit genes and small subunit genes from different species, and the recombinant gene expression vectors are obtained in a highly-controllable manner; escherichia coli glgC mutant strains are converted and inoculated to a Conberg enrichment solid culture medium, and iodine-potassium iodide dyeing is used to screen the high-activity ADP-glucose pyrophosphorylase mutant. The screening method is simple and practical and stable and reliable. The enzyme activity and glycogen content of the ADP-glucose pyrophosphorylase mutant are evidently higher than those of wild corn AGPase, evident change of the substrate affinity of the ADP-glucose pyrophosphorylase mutant is avoided, and the sensitivity of the ADP-glucose pyrophosphorylase mutant to activating agent is increased.

The invention discloses a corn endosperm ADP-glucose pyrophosphorylase mutant, a screening method and application thereof. The screening method comprises the following steps: 1) using hydroxylamine hydrochloride mutagen to randomly mutate the large and small subunit cDNA of corn endosperm AGPase under the condition of 70-80°C warm bath; The wild-type maize endosperm AGPase large and small subunit cDNAs were transformed into Escherichia coli glgC mutant strains, and positive single clones were screened; 3) The positive clones were streaked and inoculated on a Conberg enriched solid culture plate, and cultured at 35-39°C; 4) Use iodine-potassium iodide solution to stain the single colony grown on the Conberg enriched solid culture plate, and compare the color with the control colony. The mutant strain is darker than the control color. After sequencing verification, the corn endosperm ADP- Glucose pyrophosphorylase mutants. The maize endosperm ADP-glucose pyrophosphorylase mutant and its screening method of the present invention will play an important role in the improvement of maize varieties.

The invention discloses genetically engineered bacteria and application thereof to preparation of rebaudioside A. The genetically engineered bacteria capable of producing stevia rebaudiana glycosyl transferase UGT76G1 and UDP-glucose pyrophosphorylase UGPase are obtained through construction, and perform overexpression on the UDP-glucose pyrophosphorylase UGPase on the basis of performing constitutive expression on recombinant bacteria of the stevia rebaudiana glycosyl transferase UGT to increase the glycosyl donor quantity of glycosylation reaction. A whole-cell catalytic reaction system is established, and stevioside is converted into the rebaudioside A. The dosage concentration of the bacteria is 1 to 10 g / L, the dosage of glucose is 20 to 120 g / L and the dosage of the substrate stevioside is 1 to 20 g / L. In the catalytic reaction process, a cell catalyst is subjected to surfactant treatment, the cell permeability is improved and the yield of the rebaudioside A is greatly increased. A synthetic route of intracellular UDPG is utilized, so the glycosyl donor quantity of the intracellular UDPG is increased, additional addition is avoided and regeneration cycle is realized; and cultivation of yeast and expression of recombinase are conducted simultaneously, so the cycle of the cultivation of the recombinant bacterial and the expression of the recombinase is shortened.

Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding ADP-glucose pyrophosphorylase small subunit (AGPaseSS) proteins, polypeptides encompassing AGPaseSS proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.