The disclosure relates to the field of
molecular pathology (for example,
cancer diagnosis, prognosis, treatment and / or therapy prediction) through the detection of
RNA, mutations, copy number changes and determination of the
methylation status of specific sequences of the
genome of individual patients in hybridization assays (
southern blot, ISH,
dot blot) including in situ determination of the
methylation status of specific sequences of the
genome of individual patients in individual cells. More specifically, this disclosure relates to: a) target-specific probes covalently attached to a labeled
tail, b) the synthesis method of said the probe, c) the usage of said the probe such as an
in situ hybridization-based method to correlate the
methylation status of a
promoter region of a
gene in a
biopsy or
cytology specimen of a patient to the morphology and localization in that specimen, and d) kits comprising the target-specific probes. The latter method and products allow detection of (epi) genetic changes in specific
cell types of histological or cytological (
cancer) specimens or on membranes that will contribute to scientific research and that will help physicians to accurately diagnose diseases and / or start an appropriate treatment.