Use of mixed duplex oligonucleotides to effect localized genetic changes in plants
a technology of mixed duplex oligonucleotides and genetic changes, which is applied in the direction of peptide sources, applications, peptides, etc., can solve the problems of non-regenerable tissue, and the inability to disclose the use of mdon to make genetic changes
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example 1
Male Sterility
[0051] Certain commercially grown plants are routinely grown from hybrid seed including corn (maize, Zea maize), tomatoes and most other vegetables. The production of hybrid seed requires that plants of one purebred line be pollinated only by pollen from another purebred line, i.e., that there be no self pollination. The removal of the pollen-producing organs from the purebred parental plants is a laborious and expensive process. Therefore, a mutation that induces male-sterility i.e., suppresses pollen production or function, would obviate the need for such process.
[0052] Several genes have been identified that are necessary for the maturation or function of pollen but are not essential for other processes of the plant. Chalcone synthase (chs) is the key enzyme in the synthesis of flavonoids, which are pigments found in flowers and pollen. Inhibition of chs by the introduction of a chs antisense expressing gene in the petunia results in male sterility of the plant. V...
example 2
Alteration of Carbohydrate Metabolism of Tubers
[0055] Once harvested, potato tubers are subject to disease, shrinkage and sprouting during storage. To avoid these losses the storage temperature is reduced to 35-40° F. However, at reduced temperatures, the starch in the tubers undergoes conversion to sugar, termed “cold sweetening”, which reduces the commercial and nutritional value of the tuber. Two enzymes are critical for the cold sweetening process: acid invertase and UDP-glucose pyrophosphorylase. Zrenner, R., et al., 1996, Planta 198, 246-252 and Spychalla, J. P., et al., 1994, J. Plant Physiol. 144, 444-453, respectively. The sequence of potato acid invertase is found in EMBL database Accession No. X70368 (SEQ ID NO. 1) and the sequence of the potato UDP Glucose pyrophosphorylase is reported be Katsube, T. et al., 1991, Biochem. 30, 8546-8551. Accordingly, the present embodiment of the invention provides for a method of preventing cold sweetening by the interruption of the ac...
example 3
Reduction in Post Harvest Browning Due to PPO
[0056] Polyphenol oxidase (PPO) is the major cause of enzymatic browning in higher plants. PPO catalyzes the conversion of monophenols to o-diphenols and of o-dihydroxyphenols to o-quinones. The quinone products then polymerize and react with amino acid groups in the cellular proteins, which results in discoloration. The problem of PPO induced browning is routinely addressed by the addition of sulfites to the foods, which has been found to be associated with some possible health risk and consumer aversion. PPO normally functions in the defense of the plant to pathogens or insect pests and, hence, is not essential to the viability of the plant. Accordingly, the present embodiment of the invention provides for a method of preventing enzymatic browning by the interruption of the PPO gene by introduction of a frameshift, one or more in-frame termination codons or by interruption of the promoter in apple, grape, avocado, pear and banana.
[005...
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