37 results about "Cinnamyl-alcohol dehydrogenase" patented technology

The invention concerns the use of duplex oligonucleotides about 25 to 30 base pairs to introduce site specific genetic alterations in plant cells. The oligonucleotides can be delivered by mechanical (biolistic) systems or by electrpoporation of plant protoplasts. Thereafter plants having the genetic alteration can be generated from the altered cells. In specific embodiments the invention concerns alteration in the gene that encode acid invertase, UDP-glucose pyrophosphorylase, polyphenol oxidase, O-methyl transferase, cinnamyl alcohol dehydrogenase, ACC synthase and ACC oxidase or etr-1 or a homolog of etr-1, and plants having isolated point mutations in such genes.

The invention relates to a method for simultaneously detecting gene mutations of acetaldehyde dehydrogenase 2 and alcohol dehydrogenase 2. Concretely, a probe effective for detection of genetic polymorphism of the ALDH2 gene rs671 and the ADH2 gene rs1229984, a method for simultaneously detecting gene mutations and a kit for the purpose are provided. Thus the invention provides a probe for polymorphism detection, namely a probe for detection of at at least one type of genetic polymorphism of the ALDH2 gene rs671 and the ADH2 gene rs1229984, characterized in that: the probe comprises at least 1 type of fluorescently labeled oligonucleotide selected from (P1) to (P3') defined in the description.

The invention discloses a cinnamyl alcohol dehydrogenase of wheat and an encoding gene and application thereof; the cinnamyl alcohol dehydrogenase is the protein of the following a) or b): a) the protein composed of the amino acid sequences represented by the sequences 2 in the sequence table; b) the protein derived from a) and related to the cinnamyl alcohol dehydrogenase function by substituting and / or deleting and / or adding one or more amine acid in the amino acid sequences represented by the sequences 2 in the sequence table. The invention further provides the encoding gene of the cinnamyl alcohol dehydrogenase, and application of the encoding gene in culturing lodging-resistant plants. The encoding gene is introduced in the plants; and the transformed plant hosts can be wheat. The protein in the invention utilizes coniferyl aldehyde as base materials and has enzyme activities of 94.49 nmol / sec / mg, showing that the protein can control the lignin synthesis of the wheat. Northern hybridization is carried out on the Ta-CADI genes of the stems of easily-lodging and lodging-resistant wheat, which shows that the Ta-CADI genes not only control the lignin synthesis of the wheat, but also are closely related to the lodging-resistant characteristics of the stems of the wheat.