Method for improving quality of cotton fibers by overexpressing GhCAD6 gene

Abstract

The invention relates to a method for improving quality of cotton fibers by overexpressing GhCAD6 gene, belonging to the technical field of biology. The method comprises the following steps: constructing a plant overexpression vector pCAMINBIA-2300-GhCAD6 from styrone dehydrogenase gene GhCAD6 in cotton fibers, transforming the vector into agrobacterium LB4404 by agrobacterium mediation, and integrating into cotton to obtain the trans-styrone dehydrogenase gene cotton plant. The field and molecular detection analysis detects that the lignin content in cotton fibers obviously increases in different development periods, the fibrocyte wall thickness obviously decreases in the cotton secondary wall rapid deposition stage, and the ripe cotton fiber surface becomes more compact and smooth. The method can maximally enhance the length of the cotton fibers by 16.935%, enhance the specific strength by 25.10% and enhance the fiber yield by 3.80%, thereby greatly improving the quality of the cotton fibers.

Description

1. Technical field [0001] The invention relates to a transgenic breeding method, in particular to a method for improving cotton fiber quality by overexpressing cinnamyl alcohol dehydrogenase gene GhCAD6. 2. Background technology [0002] Cotton is one of the main economic crops in our country, and our country is also the largest producer and consumer of cotton in the world; cotton fiber is the main raw material for people's clothing and daily textiles, and occupies an important position in the national economy and people's lives. However, the quality of my country's cotton fiber is not satisfactory, and it is difficult to meet the various needs of the domestic and foreign markets and the textile industry. There are two main defects in fiber quality: one is that the fiber specific strength is relatively low, which has a certain impact on the product quality of cotton yarn; the other is that the matching of various indicators of fiber quality is unreasonable. After the mid-19...

AI Technical Summary

Problems solved by technology

However, the quality of cotton fiber in my country is not satisfactory, and it is difficult to meet the various needs of domestic and foreign markets and the textile industry.

Fiber quality mainly has two defects: one is that the fiber specific strength is relatively low, which has a certain impact on the product quality of cotton yarn; the other is that the fiber quality indicators are unreasonably matched

After the mid-1990s, my country’s cotton quality improvement entered a bottleneck period, and the specific strength did not match other physical performance indicators. Although the goal was still to focus on improving fiber strength while maintaining other quality indicators, due to the lack of excellent germplasm lines and parents materials, and lack of innovative technology, so the main quality indicators have not changed significantly

Moreover, the expression of most exogenous genes in cotton fibers cannot be well and stably inherited, so the application value in cotton production is very limited

At present, there are no relevant research and application reports

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