36 results about "Galactokinase" patented technology

The invention discloses a fluorescent probe for analyzing, testing and screening a galactokinase inhibitor. The structure of the fluorescent probe is shown in the formula (I) in the specification, wherein X is O or NH, and R2 is selected from compounds as shown in the specification. The fluorescent probe can simplify the conventional screening process of the galactokinase inhibitor and is directly used in any cell system expressing galactokinase without use of purified and refined galactokinase protein; with the application of the fluorescent probe, purposes of analyzing, testing and screening the galactosidase inhibitor can be achieved conveniently and rapidly, and an effective means and a useful tool are provided for discovery and development of lead compounds and effective drugs capable of preventing and treating the galactosemia disease.

By amplifying the upstream and downstream sequences of genome DNA of galactokinase of Rhodosporidium toruloides, a promoter and a terminator are obtained. The promoter and terminator can be widely applied to gene expression, operation of genetic engineering, and strain improvement of Rhodosoporidium, Sporidiobolus, Sporobolomyces, and Rhodotorula. The nucleotide sequences of the promoter and the terminator are SEQ ID No.1 and SEQ ID No.2 respectively. The invention also relates to a DNA expression cassette or recombinant carrier comprising the elements, a method utilizing related elements to establish gene engineering strains of Rhodosoporidium, Sporidiobolus, and Rhodotorula, and corresponding strains.

The invention discloses a method for synthesizing UDP-galactose and a method for synthesizing a galactosyl compound, and belongs to the technical field of bioengineering. The invention relates to a method for generating UDP-galactose by catalyzing UTP, ATP and D-galactose through a thermophilic enzyme method two-step reaction. The method comprises the following two steps: i) taking D-galactose and ATP as substrates, and catalytically synthesizing 1-P-Gal (1-phosphate-galactose) by using thermophilic galactokinase TTHA0595; and (ii) by taking the 1-P-Gal and the UTP as the substrates, carrying out catalytic synthesis on the UDP-galactose by using the thermophilic UDP-glucose pyrophosphorylase TTE0732. The synthesized UDP-galactose is used as a glycosyl donor, and thermophilic galactosyl transferase TON1857 can be used for further catalyzing substrate compounds such as vanillin, p-nitrophenol or silybin to synthesize the galactosyl compound, so that the method for synthesizing the galactosyl compound by the thermophilic enzyme method is constructed.

The invention relates to application of galactokinase in synthesizing N-acetylgalactose-1-phosphoric acid and derivatives thereof, belonging to the technical field of biotechnology. The application method comprises the following steps: (1) preparing a galactokinase solution from galactokinase of which the amino acid sequence is disclosed as SEQ ID No.1; (2) preparing an N-acetylgalactose solution or N-acetylgalactose derivative solution from N-acetylgalactose or N-acetylgalactose derivatives; and (3) proportionally mixing the galactokinase solution with the N-acetylgalactose solution or N-acetylgalactose derivative solution, adding MgCl2 and ATP (adenosine triphosphate), reacting, and purifying after the reaction, thereby obtaining the N-acetylgalactose-1-phosphoric acid or derivatives thereof. The invention overcomes the technical defect that the galactokinase can not be applied to actual production since the galactokinase can not be synthesized into N-acetylgalactose-1-phosphoric acid or the synthesis efficiency of N-acetylgalactose-1-phosphoric acid is very low in the prior art.

The present invention relates to a Streptococcus thermophilus strain, wherein the strain is galactose-fermenting, wherein the strain carries a mutation in the DNA sequence of the glcK gene encoding a glucokinase protein, wherein the mutation inactivates the glucokinase protein or has a negative effect on expression of the gene, and wherein the strain carries a mutation in the promoter region of the GalK gene encoding a galactokinase protein.

A promoter and a terminator which can be widely applied to gene expression, genetic engineering operation and strain improvement of Rhodosoporidium, Sporidiobolus, Sporobolomyces and Rhodotorula in red yeast through amplifying the upstream and downstream sequences of Rhodosporidium toruloides galactokinase genome DNA and carrying out biological information analysis and functional verification, the nucleotide sequence of the promoter is represented by SEQ ID NO:1, and the nucleotide sequence of the terminator is represented by SEQ ID NO:2. The invention also relates to DNA expression box or a recombinant vector containing above elements, a method using the correlated elements to construct Rhodosoporidium, Sporidiobolus, Sporobolomyces and Rhodotorula gene engineering strains, and corresponding strains.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactose kinase, acetyl-CoA carboxylase, pyruvate dehydrogenase The series of catalytic reactions are completed, and the present invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

Disclosed are inhibitors of human galactokinase of formula (1) that are useful in treating or preventing a galactokinase mediated disease or disorder, e.g., galactosemia. Also disclosed are a composition comprising a pharmaceutically acceptable carrier and at least one inhibitor of the invention, and a method of treating or preventing such disease or disorder in a mammal. Formula (I)

The invention discloses a bovine lactoferricin peptide constitutive type yeast expression vector and a preparation method thereof. The expression vector is obtained by introducing a bovine lactoferricin peptide gene into pYES2 plasmids and utilizing a TPI (triosephosphate isomerase) promoter to substitute a GAL1 (galactokinase 1) promoter of the pYES2 plasmids. The pYES2-TPI-LfcinB recombinant plasmids constructed by the preparation method disclosed by the invention can effectively and stably secrete bovine lactoferricin peptide without using an inducer, so that production cost and production complexity are lowered.

The present invention relates to the determination method of galactose content, the composition and the component of reagent by enzyme colorimetric method and enzyme-linked method technology, the technical principle of its determination is based on galactokinase, carbamoyl phosphate synthetase, malonate semialdehyde A series of catalytic reactions of the dehydrogenase is completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactose kinase, carbamoyl phosphate synthase, hydrogen cyanide synthase The series of catalytic reactions are completed, and the present invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The present invention relates to the measuring method of galactose content using enzyme colorimetric method and enzyme-linked method technology, the composition and component of reagent, the technical principle of its measuring is based on galactokinase, acetyl coenzyme A carboxylase, formate dehydrogenase The series of catalytic reactions are completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactose kinase, carbamoyl phosphate synthetase, pyruvate dehydrogenase The series of catalytic reactions are completed, and the present invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactose kinase, pyruvate carboxylase, malonate semialdehyde desorption The series of catalyzed reactions of the hydrogenase is completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, the technical principle of which is based on galactose kinase, pyruvate carboxylase, phosphogluconate dehydrogenation A series of catalyzed reactions of the enzyme is completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a determination method for the content of ammonia (ammonia ions) by using an enzymatic colorimetric technology and an enzymatic couple reaction and to composition and components of a reagent. A technical principle used in the method is that determination is realized through series reactions of ammonia kinase, galactokinase and galactose dehydrogenase. The invention also relates to an ammonia (ammonia ion) diagnostic / assay kit. The determination method provided in the invention has high sensitivity and a small error; the determination method and the kit in the invention can be widely applied in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of the measurement is based on galactose kinase, carbamoyl phosphate synthase, phosphogluconate The series of catalyzed reactions of the hydrogenase is completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactose kinase, carbamoyl phosphate synthetase, malate dehydrogenase The series of catalytic reactions are completed, and the present invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactose kinase, acetyl-CoA carboxylase, hydrogen cyanide synthase The series of catalytic reactions are completed, and the present invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention discloses a fluorescent probe for analyzing, testing and screening a galactokinase inhibitor. The structure of the fluorescent probe is shown in the formula (I) in the specification, wherein X is O or NH, and R2 is selected from compounds as shown in the specification. The fluorescent probe can simplify the conventional screening process of the galactokinase inhibitor and is directly used in any cell system expressing galactokinase without use of purified and refined galactokinase protein; with the application of the fluorescent probe, purposes of analyzing, testing and screening the galactosidase inhibitor can be achieved conveniently and rapidly, and an effective means and a useful tool are provided for discovery and development of lead compounds and effective drugs capable of preventing and treating the galactosemia disease.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on the development of galactose kinase, pyruvate carboxylase, and hydrogen cyanide synthase. The series of catalytic reactions are completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The present invention relates to the measuring method of the galactose content of utilizing enzyme colorimetric method and enzyme-linked method technology, the composition and the composition of reagent, the technical principle of its measuring is based on galactokinase, acetyl coenzyme A carboxylase, malonate semialdehyde A series of catalytic reactions of the dehydrogenase is completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on the development of galactose kinase, pyruvate carboxylase, and pyruvate dehydrogenase. The series of catalytic reactions are completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, the technical principle of which is based on the series of galactose kinase, pyruvate carboxylase and formate dehydrogenase After the catalytic reaction is completed, the present invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

The present disclosure describes a genetically engineered bacteria that relieves the catabolite repression problem exerted by the Spot 42 small regulatory RNA by adding a galactokinase that does not contain the Spot 42 binding region. As such, galK and galM and the like can be expressed allow better galactose utilization.

By amplifying the upstream and downstream sequences of genome DNA of galactokinase of Rhodosporidium toruloides, a promoter and a terminator are obtained. The promoter and terminator can be widely applied to gene expression, operation of genetic engineering, and strain improvement of Rhodosoporidium, Sporidiobolus, Sporobolomyces, and Rhodotorula. The nucleotide sequences of the promoter and the terminator are SEQ ID No.1 and SEQ ID No.2 respectively. The invention also relates to a DNA expression cassette or recombinant carrier comprising the elements, a method utilizing related elements to establish gene engineering strains of Rhodosoporidium, Sporidiobolus, and Rhodotorula, and corresponding strains.

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on the development of galactose kinase, carbamoyl phosphate synthase and formate dehydrogenase The series of catalytic reactions are completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.