The invention discloses a function and application of thymidylate synthetase CAD (carbamoyl-
phosphate synthetase II, aspartate transcarbamylase and dihydroorotase) genes in treating
fatty liver and type-II
diabetes mellitus, and belongs to the field of
gene functions and application. Functions of CAD genes are studied by a high-
fat diet (HFD) induced model, and results discover that the levels of
body weight and fasting blood-glucose of a CAD
gene knock-out mouse in an HFD group are higher than those of a WT (
Wild Type) mouse in a control group; glucose tolerance tests by
intraperitoneal injection discover that the tolerance of the CAD
gene knock-out mouse on glucose is remarkably weakened; the integral liver appearance,
liver weight, liver / weight ratio and lipid component
pathological staining result of the mouse indicate that the
fatty liver disease of the CAD knock-out mouse in the HFD group is remarkably severe, the
lipid accumulation is remarkably increased, and these results indicate that CAD gene knock-out can remarkably deteriorate
fatty liver and type-II
diabetes mellitus. Regarding the effects of CAD, the CAD can be used for preparing medicaments for preventing, relieving and / or treating fatty liver and / or type-II
diabetes mellitus.