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Glycine Determination Method and Glycine Determination Kit

A technology of glycine aminotransferase and glycine, which is applied in the field of glycine determination methods and kits thereof, and can solve problems such as non-use of glycine

Inactive Publication Date: 2011-12-28
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to my country's "Hygienic Standards for the Use of Food Additives" GB2760, as a food additive, glycine is only allowed to be used in flavoring agents and soy milk, and the maximum limit is 1 gram per kilogram, but it is not allowed to be used in milk-containing beverages

Method used

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  • Glycine Determination Method and Glycine Determination Kit
  • Glycine Determination Method and Glycine Determination Kit
  • Glycine Determination Method and Glycine Determination Kit

Examples

Experimental program
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Effect test

Embodiment 1

[0130] Embodiment 1: Determination of glycine content in milk

[0131] 1) Preparation of standard samples

[0132] Dissolve a certain amount of glycine in water or buffer, and then adjust the concentration of glycine to 1 mmol / L;

[0133] 2) Pretreatment of samples to be tested

[0134] The milk sample is used as the sample to be tested without pretreatment;

[0135] 3) Blank sample

[0136] Described water or buffer solution are used as blank sample, and its glycine concentration is 0 mmol / L;

[0137] B. Preparation of reagent solution:

[0138] The glycine determination reagent of the present embodiment is a single-dose reagent, which contains:

[0139] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0140] Glycerin 1mol / L

[0141] NADPH0.25mmol / L

[0142] Glycine aminotransferase 16000U / L

[0143] Carbamyl phosphate synthase 18000U / L

[0144] Malonate semialdehyde dehydrogenase 12000U / L

[0145] Ketoglutarate 15mmol / L

[0146] Adenosine diph...

Embodiment 2

[0162] Embodiment 2: the mensuration of glycine content in the milk

[0163] 1) Preparation of standard samples

[0164] Dissolve a certain amount of glycine in water, then adjust the concentration of glycine to 1 mmol / L, as a standard sample;

[0165] 2) Preparation of samples to be tested

[0166] The milk sample is used as the sample to be tested and does not need to be processed;

[0167] 3) Blank sample

[0168] Described water is used as blank sample, and its glycine concentration is 0 mmol / liter;

[0169] B. Preparation of reagent solution:

[0170] Glycine Assay Reagent is a two-part reagent containing:

[0171] Reagent 1 with the following composition:

[0172] Phosphate buffer 100mmol / L

[0173] NADH / NAD + 0.25mmol / L

[0174] Ketoglutarate 15mmol / L

[0175] Adenosine diphosphate 5mmol / L

[0176] Monovalent phosphate 15mmol / L

[0177] Carbamoyl phosphate 5mmol / L

[0178] Acetyl-CoA 15mmol / L;

[0179] Reagent 2 with the following composition: ...

Embodiment 3

[0201] Embodiment 3: the mensuration of glycine content in the milk sample

[0202] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:

[0203] B. Preparation of reagent solution:

[0204] Glycine Assay Reagent is a three-part reagent that contains:

[0205] Reagent 1 with the following composition:

[0206] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0207] NADH0.25mmol / L

[0208] Ketoglutarate 15mmol / L

[0209] Adenosine diphosphate 5mmol / L

[0210] Monovalent phosphate 15mmol / L

[0211] Carbamoyl phosphate 5mmol / L

[0212] Acetyl-CoA 15mmol / L;

[0213] Reagent 2 with the following composition:

[0214] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0215] Ammonium sulfate 1mol / L

[0216] Carbamyl phosphate synthase 28000U / L

[0217] Malonate semialdehyde dehydrogenase 50000U / L;

[0218] Reagent 3 with the following composition:

[0219] Tris(carboxymethyl)aminomethane-hydrochl...

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Abstract

The invention relates to a method for measuring glycine content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on glycine transaminase, carbamoyl phosphate synthetase, malonate semialdehyde dehydrogenation A series of catalyzed reactions of the enzyme is completed, and the invention also relates to a glycine determination kit. The determination method of the invention has high sensitivity and small error, so the determination method and the kit of the invention can be widely used in food inspection.

Description

【Technical field】 [0001] The invention relates to the technical field of food inspection and determination, more specifically, the invention relates to a glycine determination method and a kit thereof. 【Background technique】 [0002] Glycine is a non-essential amino acid. Glycine has a unique sweet taste, which can ease the acid and alkali taste, cover the bitter taste of saccharin added in food and enhance the sweet taste. If the human body consumes too much glycine, not only will it not be absorbed and utilized by the human body, but it will also break the body's absorption balance of amino acids and affect the absorption of other amino acids, resulting in nutritional imbalance and affecting health. Glycine cannot be used by the body, it can only be decomposed and converted into heat energy, which will increase the burden on the liver. The nitrogen-containing compounds in the decomposition products have to be excreted through the urine, which will increase the burden on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31G01N21/33C12Q1/52C12Q1/32C12Q1/25
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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