The invention relates to a method and reagent for immunological detection of a homogeneous enzyme determination technology, and is used for determination of the ADMA (N<G>,N<G>,dimethyl-L-arginine, asymmetric dimethylarginine, ADMA) content in a detected sample. According to the invention, anti-ADMA specific antibodies and enzyme-ADMA complexes are used, the immunological reaction of the enzyme-ADMA complexes and the anti-ADMA specific antibodies is utilized, after antigen antibody complexes are formed, the activity of the enzyme-ADMA complexes is inhibited, the changes of enzymatic activity in a reaction system is determined to obtain the number of combined enzyme markers, so as to obtain the content of ADMA in the detected sample. The method has the advantage of capability of realizing immunoassay as the common biochemical enzyme method, so as to simultaneously determine multiple samples on a full-automatic biochemical analyzer.