The invention discloses a method for preparing immunoactive peptide by taking red pine seed meal as a raw material, belonging to the technical field of agricultural product deep processing and comprehensive utilization of byproducts thereof. The red pine seed meal immunoactive peptide with molecular weight of 3,000-10,000 Da, protein content of 92-96 percent, moisture of 2-4 percent, 0.5-1.5 percent of ash content, 0.40-0.60 percent of lymphocyte conversion value and 45-65 percent of NK (Natural Killer) cell activity is obtained through raw material pretreatment, complex enzyme controllable enzymolysis, membrane separation, vacuum concentration and vacuum freeze drying processes, and immunoactive evaluation is performed by using lymphocyte conversion and NK cell activity measurement. The selected red pine seed meal is originated from the byproducts produced after oil expression of the red pine seed meal by a cold expression method, and is not in contact with any organic extraction solvent, the red pine seed meal immunoactive peptide is conveniently and effectively separated, and the original quality and functions of the red pine seeds of Changbai mountains are maintained.