The invention discloses a method for distributing and routing optimal services of multi-layer satellite network based on minimum time delay, and aims to solve the problems of large end-to-end time delay, low handling capacity and insufficient utilization of network resource of a multi-layer satellite communication network routing method. The method comprises the following steps: determining a logic area, a virtual node number and a low-earth-orbit satellite routing list according to the initial network topology; periodically updating the node number and the routing list by a low earth orbit; updating a virtual satellite set and a member routing list according to the snapshot time sequence set by a medium earth orbit, and synchronously updating the number of an administrator by the low earth orbit; if the services arrive and the arrival rate of the current satellite area is less than the arrival rate threshold of the ground service supported by the area based on the minimum end-to-end time, transmitting the service in the low earth orbit, and otherwise, transferring the service to a high-level satellite to route, and finally sending a target node. With the adoption of the method, the performance of the multi-layer satellite communication network is improved; the method can be applied to the routing of the multi-layer satellite communication network.