109results about How to "Sensitive measurement" patented technology

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

Methods and devices for enhanced ultrasound detection based upon changing temperature and ultrasound reflectivity of a temperature-dependent contrast agent bound to an ultrasound target are disclosed. The methods and devices can be used for enhanced imaging alone or in conjunction with drug delivery, with therapeutic approaches such as hyperthermia or cryotherapy or with other imaging modalities.

The invention is based on an HVAC (heating, ventilation and air-conditioning) system which comprises the following components: a) a fluid flow duct (1), b) a fluid flow valve (7) which is arranged therein and has a valve body (5) in the fluid flow duct (1) and a valve motor (15) which moves the valve body (5), c) a control circuit for activating the valve motor, d) a sensor (8) in the fluid flow duct (1) and e) an evaluation module for evaluating signals of the sensor. In order to produce an automated functional control, the following procedure is adopted: f) a first actuation signal is preset for the valve motor by the control circuit, and the actuation signal corresponds to a first setpoint position of the valve body (5), g) registration of a first signal of the sensor (8) by the evaluation module, and h) determination of a functional diagnosis of the fluid flow valve on the basis of the first signal of the sensor.

The invention belongs to the technical field of flexible electron sensors, and particularly relates to a wearing type flexible pressure sensor and a preparing method thereof. The wearing type flexible pressure sensor comprises five parts of an upper support substrate, a super soft Ecoflex pressure detection head, PVDF piezoelectric thin film, an array form micro boss support structure and a lower support substrate. The super flexible Ecoflex pressure detection head can sense pressure to deform, and further compress the PVDF piezoelectric thin film to output an electric signal. The invention further discloses the preparing method of the wearing type flexible pressure sensor. The super soft Ecoflex pressure detection head can very sensitively sense weak pulse visual skipping of a human body, and solve the problems that wearable intelligent equipment cannot measure a pressure signal and is not flexible. The wearing type flexible pressure sensor further has the advantages of being small and exquisite in size, comfortable to wear, long in service life, low in preparation cost and the like, and being suitable for being used in large-scale production.

The invention is related to a laser diode based multiple beam laser spot imaging system for characterization of vehicle dynamics. A laser diode based, preferably VCSEL based laser imaging system is utilized to characterize the vehicle dynamics. One or more laser beams are directed to the road surface. A compact imaging system including an imaging matrix sensor such as a CCD or CMOS camera measures locations or separations of individual laser spots. Loading status of vehicles and vehicles' pitch and roll angle can be characterized by analyzing the change of laser spot locations or separations.

A detection system combines an excitation radiation source (18) providing excitation radiation (10) to an analysis region of a sample (14) within a substrate (16) having a detection surface, a detector (22) for detecting radiation collected from the analysis region comprising the detection surface of the sample resulting from the excitation, and a magnet arrangement (24) beneath the analysis region of the sample, and stationary with respect to the excitation radiation source and light coupling arrangement, for attracting magnetic beads (15) within the sample to the substrate surface The detection radiation is collected from the detection surface of the substrate, to give an enhanced surface specificity The invention combines the advantages of surface detection with a simple low cost magnetic system for bringing the target to the surface

Embodiments of the invention relate to resonant circuits; particularly but not exclusively the embodiments relate to resonant circuits in RPID (radio frequency identification) responsive to a wide frequency range. A controllable electric resonator comprising an inductor coupled to a first capacitor to form a resonant circuit, the resonator further comprising a controllable element, a second capacitor controllable coupled across said first capacitor by said controllable element, and a control device to control said controllable element such that a total effective capacitance of said first and second capacitor varies over a duty cycle of an oscillatory signal on said resonator.

In a microsensor with a micro-electromechanical sensor element and an integrated circuit for measuring, calibration and compensation electronics, whereby the sensor is connected electrically to the integrated circuit (IC), the micro-electromechanical sensor element is arranged directly on the integrated circuit with accurate positioning, and is connected with electric conductivity via a circulating soldered joint.

A non-invasive electro-optic probe and probe method for probing electric fields in semiconductor devices comprising directing laser output at a semiconductor device to be probed employing a laser having an operating wavelength such that photon energy of a second harmonic wavelength of the operating wavelength is below the band gap of a semiconductor comprised by the semiconductor device and detecting second harmonic wavelength but not operating wavelength radiation from the semiconductor device.

The present invention is directed to a non-immunogenic cellular composition comprising: a cell having a cell surface and antigenic determinants on the cell surface; an optional linker molecule covalently attached to the cell surface; and a hydrophilic, biocompatible, non-immunogenicity providing compound or polymer (e.g., polyethylene glycol or a derivative thereof) covalently attached to the linker molecule or directly to the cell. In one embodiment, the linker molecule is covalently attached directly to the antigenic determinant on the cell surface. In an alternate embodiment, the linker molecule may be covalently attached to a non-antigenic site on the cell surface, but will camouflage the antigenic determinant on the cell surface. Various uses of the resulting non-immunogenic cell are also provided, including a method of decreasing phagocytosis of a cell, a method of decreasing an adverse reaction to a transfusion, a method of decreasing rejection of a transplanted cell, tissue or organ, and a method of decreasing antibody-induced aggregation of cells.

The invention provides an ATR spectrum measurement type Fourier transform spectrometer based on integrated optical waveguide. The spectrometer comprises an integrated optical waveguide electro-optical modulator, a voltage function generator, a broadband light source, a photoelectric detector, a signal processing chip and a fiber having an ATR sensitive window. According to the invention, light from the light source is coupled into the fiber, passes through an ATR sensitive window zone along the fiber and coupled into the integrated optical waveguide electro-optical modulator; voltage varying with time is applied on the integrated optical waveguide electro-optical modulator by using the voltage function generator, the intensity of output light of the integrated optical waveguide electro-optical modulator is measured by using the photoelectric detector simultaneously, and the signal processing chip connected with the photoelectric detector and the voltage function generator carries out Fourier transform processing on measured optical signals so as to obtain the spectrum of incident light. The spectrometer provided in the invention can determine visible-infrared absorption spectrum of solid, liquid and monomolecular layers adsorbed on the surface of the ATR sensitive window, has strong anti-interference capability and is applicable to on-site rapid quantitative detection.

A multi-well helium Dewar is provided for recirculating coolant about a cryostat probe; the Dewar includes a first well containing a first coolant reservoir, and a second well containing a second coolant reservoir. A fluid connection extends between the first and second coolant reservoirs. A low impedance conduit further connects a top end of the second well to the first well. In this regard, the Dewar at least partially contains a cryocooler within the first well and a cryostat probe within the second well. Vibrational noise is reduced by the incorporation of soft mounting components for connecting various features. A thermal shield of the Dewar can be thermally connected to isolated components for additional cooling power and increased efficiency during initialization. A second fluid connection may include a valve attached to a counter-flow heat exchanger for efficiently re-condensing excess gas within the Dewar.

A method of concentrating a gaseous substance includes the steps of isolating a trap in which an analyte to be concentrated has been absorbed from a carrier gas passed through the trap and reducing the pressure within the trap to a first pressure. The analyte is desorbed from the trap and the analyte released from the trap is diffused into an analyser operating at a second pressure lower than the first pressure.

The invention discloses a micro thrust measurement device with an adjustable and high-magnetism gathering damper, which comprises a target system, a displacement sensor system, a target mounting block and a damping system, wherein in the target system, connection and positioning between an elastic beam and the target mounting block is realized through vertical angle aluminum, the verticality of the elastic beam is ensured, and the target is arranged at the bottom end of the elastic beam; in the displacement sensor system, a sensor mounting rod is vertically arranged, the top end is fixed with the bottom surface of the target mounting block, and the bottom end of the sensor mounting rod is provided with the displacement sensor via a sensor mounting plate; in the magnetic damping system, a magnetic stripe is formed by stripe magnets and soft iron blocks, the magnetism gathering effect is good, and the magnetic stripe is arranged on a base with an adjustable height to enable the damping force of the damper to be adjustable conveniently. The micro thrust measurement device with the damping system has the advantages that the thrust frame movable frame can be ensured to be quickly stabilized via the damper, the experiment time can be reduced, the experiment errors can be reduced, and the normal operation by the thruster is not disturbed.

A method for designing a two-dimensional array overlay target set comprises the steps of: selecting a plurality of two-dimensional array overlay target sets having different overlay errors; calculating a deviation of a simulated diffraction spectra for each two-dimensional array overlay target set; selecting a sensitive overlay target set by taking the deviations of the simulated diffraction spectra into consideration; and designing a two-dimensional array overlay target set based on the structural parameters of the sensitive overlay target set.

The invention discloses a micro mechanical differential capacitive pressure gauge. Two capacitors of the pressure gauge are formed by three capacitive polar plates: an upper polar plate formed through depositing a metal on a glass sheet or a silicon wafer; a lower polar plate formed by heavily doped silicon and an intermediate movable polar plate which is partly connected with a pressure-sensitive membrane and formed by monocrystal silicon or polycrystalline silicon. The pressure gauge is based on a basic silicon cup structure in a pressure gauge structure, when a pressure is applied to the sensitive membrane, the membrane is deformed and the intermediate polar plate connected with the sensitive membrane is also deformed so that the capacitances of the upper and lower capacitors are changed and thus differential signal output is formed. The preparation process of the pressure gauge is simple and advantages of a differential structure can be used to realize differential detection of pressure.

A MEMS transducer configured to operate as a microphone, the MEMS transducer comprising a flexible membrane, the flexible membrane having a first surface and a second surface, wherein the first surface of the flexible membrane is fluidically isolated from the second surface of the flexible membrane. Also, a MEMS device comprising a MEMS transducer, an electronic device comprising a MEMS transducer and / or a MEMS device, and a method for forming a MEMS device.

Methods and devices for enhanced ultrasound detection based upon changing temperature and ultrasound reflectivity of a temperature-dependent contrast agent bound to an ultrasound target are disclosed. The methods and devices can be used for enhanced imaging alone or in conjunction with drug delivery, with therapeutic approaches such as hyperthermia or cryotherapy or with other imaging modalities.

The invention discloses an ultrashort pulse time width measurement device and method based on spectral interference. The device comprises a to-be-measured ultrashort pulse laser, a 50:50 beam splitter, a measurement optical path, a reference optical path, a beam combiner and a spectral analyzer. According to the method, a to-be-measured ultrashort pulse is divided into two to-be-measured ultrashort pulse beams with equal power; the to-be-measured ultrashort pulse beams are emitted to the beam combiner through the measurement optical path, time delay of the to-be-measured ultrashort pulse in the measurement optical path is regulated, and the polarization state of the to-be-measured ultrashort pulse in the measurement optical path is regulated to be matched with a to-be-measured ultrashort pulse in the reference optical path; the optical power of the to-be-measured ultrashort pulse in the reference optical path is regulated to be equal to the optical power of the to-be-measured ultrashort pulse output by the measurement optical path; and the change of the spectral interference oscillation degree of the to-be-measured ultrashort pulse is measured, and the time width of the to-be-measured ultrashort pulse is calculated. Through the ultrashort pulse time width measurement device and method, the mode of measuring frequency-doubled signal intensity of the to-be-measured ultrashort pulse based on a frequency doubling crystal in traditional ultrashort pulse time width measurement is abandoned, measurement sensitivity is high, and meanwhile good applicability is achieved.

The invention relates to MFPW radar level gauging with a distance approximation. The level gauging includes an approxiamtion of a distance to the surface of a product kept in a tank. The approxiamtion is determined by relating an amplitude of a first harmonic of an IF signal with an amplitude of a second harmonic of the IF signal. Basically, each harmonics represents a given distance range. By determining the received power in two or more harmonics, and correlating them to each other, the distance may be estimated. Depending on modulation and other parameters the distance dependence may be very different, and may be selected to suit the application.

The invention belongs to the technical field of inorganic material application, and discloses a preparation method and application of a dual-function biosensor HRP@ZIF-8 / DNA. According to the invention, a biomineralization method is adopted to embed horseradish peroxidase in a pore structure of a metal organic framework ZIF-8, thereby realizing immobilization of the enzyme; in addition, good affinity to fluorophore-labeled single-stranded DNA (ssDNA) is conferred by ligand rich in pi electrons and partially coordinated metal ions through pi-pi stacking and electrostatic interaction, making itan excellent fluorescence quencher. According to the invention, the carrier function and the fluorescence quenching property of ZIF-8 are combined for the first time to develop a HRP@ZIF-8 / DNA hybridsystem for a fluorescence detection of Hg2+ and a visual detection of phenol. The sensor of the invention has a detection limit of 0.22 nM to mercury, and meanwhile, the sensor is used as a colorimetric sensor to detect phenol at a micromolar level, and the detection method is economic, effective, highly selective and sensitive.

A method and apparatuses for determining the total content of proteases by means of measuring their enzyme activity after previous deinhibition is disclosed. In biological samples, lysosomal proteases are completely (e.g. in blood serum) or partly (e.g. in tissue homogenates) inhibited. A method proposed for the deinhibition entails immersing a solid support material (e.g. nylon or nitrocellulose) as plastic strip to which is linked, covalently or by adsorption, an inhibitor-binding substance which binds the inhibitor corresponding to the protease more strongly than the protease in the sample for measurement. After the deinhibition has taken place, the plastic strip is removed from the liquid sample for measurement, and the sample for measurement is passed on for measurement of the enzyme activity. Fluorogenic substrates from which the fluorogen 7-amino-4-trifluoromethylcoumarin is eliminated proved to be particularly advantageous for the activity measurement. These substrates make it possible for the sensitivity on measurement in microtitre plates with a fluorescence reader to be at least 10 times higher compared with conventional AMC substrates in blood serum, and thus for the fluorimetric determination of such enzyme activities in blood serum to be efficient with this measuring arrangement which is widely used in clinical laboratories.

The invention relates to a device for monitoring the temperature of an oil tank by FBGs (Fiber Bragg Grating) and belongs to the technical field of FBG detection. The device comprises an ABE (Amplified Spontaneous Emission) light source, a spectrum demodulation instrument, a computer and the like and is characterized in that the ASE light source is connected with the input end of a 3db coupler by an optical fiber; one output end of the 3db coupler is connected onto an optical switch by an optical fiber; the output end of the optical switch is connected with a splitter by an optical cable; the output end of the splitter is connected with a plurality of FBGs by optical cables; the other output end of the 3db coupler is connected onto the spectrum demodulation instrument by an optical fiber; the spectrum demodulation instrument is connected with the computer; and the computer is connected with an alarm. According to the device disclosed by the invention, the temperature calibration is firstly carried out and then the temperature of the oil tank is timely monitored; and the device has the advantages of rapidness, accuracy, sensitivity, safety and stability for measuring and capability of simultaneously measuring the oil temperature and the oil gas temperature by the computer and can replace an original complex measurement instrument easy to influence by external factors, so that the cost is reduced.

The invention relates to the determination of resistances of microorganisms which produce β-lactamases, in particular “extended spectrum β-lactamases” (ESBL). The invention provides a method whereby the microbial resistance can be measured very simply and quickly by means of the catalytic effect of the microbially produced β-lactamases on β-lactam antibiotics, which consists in a hydrolytic cleavage of the β-lactam ring. The method determines the resistance of the bacteria a few hours after a suitable substrate, either a β-lactam antibiotic or a customized β-lactam derivative, has been added to a suspension of the microbes, by direct mass spectrometric measurement of the substrate breakdown caused by the β-lactamases.

The invention belongs to the technical field of online monitoring of partial discharge of electric power paper oil insulation electrical equipment and relates to a method for detecting the partial discharge direction of a compound eye type optical fiber EFPI. A system for detecting the partial discharge direction of the compound eye type optical fiber EFPI is utilized; voltage signals output by five identical amplifiers are connected to an oscilloscope, power is supplied, a compound eye type optical fiber external cavity type Fabry-Perot sensor is placed at the top end of an insulating rod, and the insulating rod is rotated toward an optical fiber external cavity type Fabry-Perot sensing head with the strongest signal till the partial discharge pulse amplitude of an optical fiber external cavity type Fabry-Perot sensing head located in the middle is the largest and the partial discharge pulse amplitudes of optical fiber external cavity type Fabry-Perot sensing heads placed on the periphery are roughly equal; then, the orientation of the optical fiber external cavity type Fabry-Perot sensing head located in the middle in the compound eye type optical fiber external cavity type Fabry-Perot sensor is the direction of the partial discharge point. The method is high in sensitivity, good in directivity and high in anti-interference capacity and can be used for locating the partial discharge direction of key portions of electrical equipment conveniently and accurately.