Glycine Determination Method and Glycine Determination Kit
A glycine and reagent technology, applied in the field of glycine determination method and its kit, can solve the problem of not allowing use and the like
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Embodiment 1
[0123] Embodiment 1: Determination of glycine content in milk
[0124] 1) Preparation of standard samples
[0125] Dissolve a certain amount of glycine in water or buffer, and then adjust the concentration of glycine to 1 mmol / L;
[0126] 2) Pretreatment of samples to be tested
[0127] The milk sample is used as the sample to be tested without pretreatment;
[0128] 3) Blank sample
[0129] Described water or buffer solution are used as blank sample, and its glycine concentration is 0 mmol / L;
[0130] B. Preparation of reagent solution:
[0131] The glycine determination reagent of the present embodiment is a single-dose reagent, which contains:
[0132] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0133] Glycerin 1mol / L
[0134] NADP + 0.75mmol / L
[0135] Hydrogen cyanide synthase 16000U / L
[0136] Carbamyl phosphate synthase 18000U / L
[0137] Glutamate synthase 12000U / L
[0138] Adenosine triphosphate 5mmol / L
...
Embodiment 2
[0152] Embodiment 2: the mensuration of glycine content in the milk
[0153] 1) Preparation of standard samples
[0154] Dissolve a certain amount of glycine in water, then adjust the concentration of glycine to 1 mmol / L, as a standard sample;
[0155] 2) Preparation of samples to be tested
[0156] The milk sample is used as the sample to be tested and does not need to be processed;
[0157] 3) Blank sample
[0158] Described water is used as blank sample, and its glycine concentration is 0 mmol / L;
[0159] B. Preparation of reagent solution:
[0160] Glycine Assay Reagent is a two-part reagent containing:
[0161] Reagent 1 with the following composition:
[0162] Phosphate buffer 100mmol / L
[0163] NAD + 1.25mmol / L
[0164] Adenosine triphosphate 5mmol / L
[0165] Glutamine 5mmol / L;
[0166] Reagent 2 with the following composition:
[0167] Phosphate buffer 100mmol / L
[0168] Ethylene glycol 5mol / L
Embodiment 3
[0188] Embodiment 3: the mensuration of glycine content in the milk sample
[0189] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:
[0190] B. Preparation of reagent solution:
[0191] Glycine Assay Reagent is a three-part reagent that contains:
[0192] Reagent 1 with the following composition:
[0193] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0194] NAD + 1.25mmol / L
[0195] Adenosine triphosphate 6mmol / L
[0196] Glutamine 4mmol / L;
[0197] Reagent 2 with the following composition:
[0198] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0199] Ammonium sulfate 1mol / L
[0200] Carbamyl phosphate synthase 28000U / L
[0201] Glutamate synthase 50000U / L;
[0202] Reagent 3 with the following composition:
[0203] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0204] Ammonium sulfate 2mol / L
[0205] Hydrogen cyanide synthas...
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