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Hydrogen peroxide sensor, preparation method thereof, and application thereof in detecting unicellular hydrogen peroxide

A hydrogen peroxide and sensor technology, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems that the sensor is not suitable for capillary electrophoresis electrochemical detection, the production of hydrogen peroxide sensor is cumbersome, and the detection limit is not low enough, so as to promote the transfer and production of electrons. Simple, high-sensitivity effects

Inactive Publication Date: 2013-07-10
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing pair H 2 o 2 detection, the sensitivity is not high, the detection limit is not low enough, and the electroactive substances that may exist in cells such as ascorbic acid and cysteine ​​have a negative effect on H 2 o 2 The measurement has interference
[0005] The Chinese patent with the application number 201110020554.4 discloses a preparation method of a nano-Co-Fe Prussian blue complex-carbon nanotube composite hydrogen peroxide sensor. The production of the hydrogen peroxide sensor in this patent is relatively cumbersome, and the sensor is too large to be suitable for capillary electrophoresis electrochemical detection.

Method used

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  • Hydrogen peroxide sensor, preparation method thereof, and application thereof in detecting unicellular hydrogen peroxide
  • Hydrogen peroxide sensor, preparation method thereof, and application thereof in detecting unicellular hydrogen peroxide
  • Hydrogen peroxide sensor, preparation method thereof, and application thereof in detecting unicellular hydrogen peroxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation method of hydrogen peroxide sensor:

[0033] (1) A platinum wire (diameter 200 μm, length about 5 cm) is penetrated into a quartz capillary tube with a length of about 3 cm, an inner diameter of 250 μm, and an outer diameter of 375 μm, and the platinum wire is exposed at both ends. Coat one end of it with JC-311 epoxy resin glue, put the platinum wire back into the capillary from the other end, fill the capillary with platinum wire coated with epoxy resin glue, and make the platinum wire just exposed to the capillary port, The copper wire polished with metallographic sandpaper and the capillary are glued together with 502 glue, and the contact length is about 1cm. Wind the exposed platinum wire on the copper wire, coat the platinum wire and copper wire with silver paste, and dry at 70°C for 30min. Insert the electrode into a quartz glass tube with a length of about 3cm, an inner diameter of about 0.5mm, and an outer diameter of about 1mm, so that the part c...

Embodiment 2

[0037] Preparation method of hydrogen peroxide sensor:

[0038] Including steps (1), (2), (3), the mass ratio of platinum nanoparticles to multi-walled carbon nanotubes in step (3) is 1:1, 1:3, 1:7, 1:9 respectively, other steps such as Example 1.

Embodiment 3

[0040] Preparation method of hydrogen peroxide sensor:

[0041] Including steps (1), (2), and (3), the concentrations of platinum nanoparticles / multi-walled carbon nanotubes in the platinum nanoparticles / multi-walled carbon nanotubes dispersion in step (2) are 3 mg / mL and 7 mg / mL respectively , 10mg / mL, other steps are as in Example 1.

[0042] Detection of hydrogen peroxide in single cells

[0043] Self-assembled capillary electrophoresis system: CHI810c electrochemical analyzer (Shanghai Chenhua Instrument Co., Ltd.); 0-30kV high voltage power supply (Shandong Normal University Instrument Factory, Shandong).

[0044] The hydrogen peroxide sensor prepared in Example 1 was used.

[0045] Experimental steps:

[0046] (1) Extraction of neutrophils: Mix 2 mL of fresh anticoagulated blood, 2 mL of whole blood and tissue homogenate dilution in a 10 mL centrifuge tube, and let stand at 20-30°C for 30 minutes. At this time, a large number of red blood cells sink to the bottom of ...

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Abstract

The invention relates to a hydrogen peroxide sensor, a preparation method thereof, and application of the hydrogen peroxide sensor in detection of unicellular hydrogen peroxide. The hydrogen peroxide sensor is prepared by loading platinum nano-particles on multi-wall carbon nanotubes by ethylene glycol reduction method and then preparing platinum nano-particle / multi-wall carbon nanotube dispersed liquid therefrom, and finally, dipping a platinum electrode with the dispersed nano-particles / multi-wall carbon nanotubes. The sensor provided by the invention is high in sensitivity, low in detection limit and wide in linear range; the sensor is taken as a detector of capillary electrophoresis; and quantitative determination of H2O2 in single cells is realized.

Description

technical field [0001] The invention mainly relates to a hydrogen peroxide sensor, which utilizes capillary electrophoresis electrochemical method to detect H in a single cell 2 o 2 , belongs to the technical field of bioelectrical analysis chemical detection. Background technique [0002] h 2 o 2 It is one of the more important metabolites in the body. It can pass through the cell membrane and is a relatively stable active oxygen. Many reports have also demonstrated that it is a second messenger of cell signaling. and H 2 o 2 It is closely related to the occurrence, development and apoptosis of tumors. 2 o 2 Assays can diagnose and prevent diseases induced by oxidative stress and damage. [0003] Many analytical methods have been used for H 2 o 2 detection, such as fluorescence, spectrophotometry, electrochemical detection and chemiluminescence. Capillary electrophoresis electrochemical analysis method is widely used in single cell analysis due to its advantages...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/447
Inventor 王晓蕾李玲君马艳芳王军刘冬菊
Owner SHANDONG NORMAL UNIV
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