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Determination method of galactose and galactose diagnosis/measurement kit

A technology of galactose and galactokinase, applied in the field of medical/food inspection and determination, can solve the problems affecting the physical and intellectual development of infants and young children, and the absorption of elements and other problems

Inactive Publication Date: 2011-12-28
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Although milk is good, not all people can enjoy it. Due to the lack of lactase, the human body cannot digest and absorb the nutrients in milk well, and will Causes absorption disorders of calcium, phosphorus, potassium, iron and other elements, affecting the physical and intellectual development of infants and young children. In the elderly, lactase deficiency is an important cause of osteoporosis

Method used

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  • Determination method of galactose and galactose diagnosis/measurement kit
  • Determination method of galactose and galactose diagnosis/measurement kit
  • Determination method of galactose and galactose diagnosis/measurement kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] Embodiment 1: the mensuration of galactose content in blood plasma

[0131] 1) Preparation of standard samples

[0132] Dissolve a certain amount of galactose in water or buffer, and then adjust the concentration of galactose to 100 micromole / liter;

[0133] 2) Preparation of samples to be tested

[0134] Plasma samples can be used directly as samples to be tested;

[0135] 3) Blank sample

[0136] The water or buffer solution is used as a blank sample, and its galactose concentration is 0 micromole / liter;

[0137] B. Preparation of reagent solution:

[0138] The galactose diagnosis / measurement reagent of the present embodiment is a single-dose reagent, which contains:

[0139] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0140] Glycerin 1mol / L

[0141] NADPH0.25mmol / L

[0142] Galactokinase 16000U / L

[0143] Pyruvate carboxylase 18000U / L

[0144] Pyruvate dehydrogenase 12000U / L

[0145] Adenosine triphosphate 5mmol / L

[0146] Monoval...

Embodiment 2

[0161] Embodiment 2: the mensuration of galactose content in blood plasma

[0162] 1) Preparation of standard samples

[0163] Dissolve a certain amount of galactose in water, and then adjust the concentration of galactose to 100 micromole / liter as a standard sample;

[0164] 2) Preparation of samples to be tested

[0165] Plasma samples can be used directly as samples to be tested;

[0166] 3) Blank sample

[0167] Described water is used as a blank sample, and its galactose concentration is 0 micromole / liter;

[0168] B. Preparation of reagent solution:

[0169] Galactose Diagnostic / Assay Reagent is a two-dose reagent containing:

[0170] Reagent 1 with the following composition:

[0171] Phosphate buffer 100mmol / L

[0172] NADH0.25mmol / L

[0173] Adenosine triphosphate 5mmol / L

[0174] Oxaloacetate 3mmol / L

[0175] Acetyl-CoA 4mmol / L;

[0176] Reagent 2 with the following composition:

[0177] Phosphate buffer 100mmol / L

[0178] Ethylene glycol 5mol / L

[0179]...

Embodiment 3

[0198] Example 3: Determination of Galactose Content in Plasma Samples

[0199] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:

[0200] B. Preparation of reagent solution:

[0201] Galactose Diagnostic / Assay Reagent is a three-dose reagent containing:

[0202] Reagent 1 with the following composition:

[0203]Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0204] NADH0.25mmol / L

[0205] Adenosine triphosphate 5mmol / L

[0206] Monovalent phosphate 15mmol / L

[0207] Oxaloacetate 3mmol / L

[0208] Acetyl-CoA 4mmol / L;

[0209] Reagent 2 with the following composition:

[0210] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0211] Ammonium sulfate 1mol / L

[0212] Pyruvate carboxylase 28000U / L

[0213] Pyruvate dehydrogenase 50000U / L

[0214] Reagent 3 with the following composition:

[0215] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0216] Ammonium sulfate ...

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Abstract

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on the development of galactose kinase, pyruvate carboxylase, and pyruvate dehydrogenase. The series of catalytic reactions are completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

Description

【Technical field】 [0001] The invention relates to the technical field of medical / food inspection and determination, more specifically, the invention relates to a galactose diagnosis / measurement method and a kit thereof. 【Background technique】 [0002] Milk is known as "liquid gold" in the nutritional field. As early as 1992, seven departments in my country jointly proposed the slogan "a glass of milk strengthens a nation". Except for cellulose, milk contains almost all kinds of nutrients needed by the human body. After lactose in milk enters the human body, it is decomposed into glucose and galactose by the action of lactase in the small intestine. Galactose is an essential substance for the development of the baby's brain and is closely related to the rapid growth of the baby's brain. [0003] Although milk is good, not all people can enjoy it. Due to the lack of lactase, some people cannot digest and absorb the nutrients in milk well, and it will also cause absorption ob...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N21/31G01N21/33C12Q1/54C12Q1/48C12Q1/32C12Q1/25
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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