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Determination method of galactose and galactose diagnosis/measurement kit

A technology of galactose and galactokinase, applied in the field of medical/food inspection and determination

Inactive Publication Date: 2011-12-28
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Although milk is good, not all people can enjoy it. Due to the lack of lactase, the human body cannot digest and absorb the nutrients in milk well, and will Causes absorption disorders of calcium, phosphorus, potassium, iron and other elements, affecting the physical and intellectual development of infants and young children. In the elderly, lactase deficiency is an important cause of osteoporosis

Method used

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  • Determination method of galactose and galactose diagnosis/measurement kit
  • Determination method of galactose and galactose diagnosis/measurement kit
  • Determination method of galactose and galactose diagnosis/measurement kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0134] Embodiment 1: the mensuration of galactose content in blood plasma

[0135] 1) Preparation of standard samples

[0136] Dissolve a certain amount of galactose in water or buffer, and then adjust the concentration of galactose to 100 micromole / liter;

[0137] 2) Pretreatment of samples to be tested

[0138] Plasma samples are used as samples to be tested without pretreatment;

[0139] 3) Blank sample

[0140] The water or buffer solution is used as a blank sample, and its galactose concentration is 0 micromole / liter;

[0141] B. Preparation of reagent solution:

[0142] The galactose diagnosis / measurement reagent of the present embodiment is a single-dose reagent, which contains:

[0143] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0144] Glycerin 1mol / L

[0145] NADPH0.25mmol / L

[0146] Galactokinase 16000U / L

[0147] Carbamyl phosphate synthase 18000U / L

[0148] Isocitrate dehydrogenase 12000U / L

[0149] Adenosine triphosphate 4mmol / ...

Embodiment 2

[0166] Embodiment 2: the mensuration of galactose content in blood plasma

[0167] 1) Preparation of standard samples

[0168] Dissolve a certain amount of galactose in water, and then adjust the concentration of galactose to 100 micromole / liter as a standard sample;

[0169] 2) Preparation of samples to be tested

[0170] Plasma samples are used as samples to be tested without pretreatment;

[0171] 3) Blank sample

[0172] Described water is used as a blank sample, and its galactose concentration is 0 micromole / liter;

[0173] B. Preparation of reagent solution:

[0174] Galactose Diagnostic / Assay Reagent is a two-dose reagent containing:

[0175] Reagent 1 with the following composition:

[0176] Phosphate buffer 100mmol / L

[0177] NADH / NAD + 0.25mmol / L

[0178] Adenosine triphosphate 4mmol / L

[0179] Glutamic acid 12mmol / L

[0180] Carbamoyl phosphate 5mmol / L

[0181] Ketoglutarate 12mmol / L;

[0182] Reagent 2 with the following composition:

[0183]...

Embodiment 3

[0204] Example 3: Determination of Galactose Content in Plasma Samples

[0205] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:

[0206] B. Preparation of reagent solution:

[0207] Galactose Diagnostic / Assay Reagent is a three-dose reagent containing:

[0208] Reagent 1 with the following composition:

[0209] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0210] NADH0.25mmol / L

[0211] Adenosine triphosphate 4mmol / L

[0212] Monovalent phosphate 15mmol / L

[0213]Glutamic acid 12mmol / L

[0214] Carbamoyl phosphate 5mmol / L

[0215] Ketoglutarate 12mmol / L;

[0216] Reagent 2 with the following composition:

[0217] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0218] Ammonium sulfate 1mol / L

[0219] Carbamyl phosphate synthase 28000U / L

[0220] Isocitrate dehydrogenase 50000U / L

[0221] Reagent 3 with the following composition:

[0222] Tris(carboxymethyl)aminomethane-hydrochlo...

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Abstract

The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on galactokinase, carbamoyl phosphate synthetase, and isocitric acid dehydrogenation A series of catalyzed reactions of the enzyme is completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.

Description

【Technical field】 [0001] The invention relates to the technical field of medical / food inspection and determination, more specifically, the invention relates to a galactose diagnosis / measurement method and a kit thereof. 【Background technique】 [0002] Milk is known as "liquid gold" in the nutritional field. As early as 1992, seven departments in my country jointly proposed the slogan "a glass of milk strengthens a nation". Except for cellulose, milk contains almost all kinds of nutrients needed by the human body. After lactose in milk enters the human body, it is decomposed into glucose and galactose by the action of lactase in the small intestine. Galactose is an essential substance for the development of the baby's brain and is closely related to the rapid growth of the baby's brain. [0003] Although milk is good, not all people can enjoy it. Due to the lack of lactase, some people cannot digest and absorb the nutrients in milk well, and it will also cause absorption o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66G01N21/31C12Q1/54C12Q1/48C12Q1/32C12Q1/25
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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