59results about How to "High flocculation activity" patented technology

The invention relates to an extra-cellular polysaccharide of aerobic Ruthia sp. strain metabolin and preparation and application thereof. An aerobic strain ZHT4-13 is separated from adhesive sludge of wild Ruditapes philippinarums in the Bohai sea offshore of China and is identified as a Ruthia sp. The aerobic strain is treated by seed culture and amplification culture, and a fermentation broth of the aerobic strain is precipitated with ethanol and separated centrifugally to obtain the extra-cellular polysaccharide MBF4-13. The extra-cellular polysaccharide MBF4-13 is measured to have high flocculation activity, the FR value to kaolin reaches over 80 percent, and the removal rate to hexavalent chromium ions reaches 69.3 percent; the extra-cellular polysaccharide MBF4-13 has high activity to decolorize high-concentration wastewater, and the decolorization ratios to methylene blue, ink blue, malachite green and crystal violet reach 86.11 percent, 99.49 percent and 97.84 percent; and the extra-cellular polysaccharide MBF4-13 plays a role of obviously improving the performance and the structure of activated sludge. The extra-cellular polysaccharide has potential value of developing a novel microbial flocculant.

The invention relates to a preparation process of preparing a flocculating agent by utilizing sludge, and discloses a method of preparing flocculating agent by utilizing sludge and application thereof. The method comprises the following steps: mutually connecting the two pretreatments of the sludge in series to change the virtual structure of the sludge, changing physical and chemical properties of the sludge particles and microbial cells in the sludge, and enabling the sludge to possess higher adsorption property of the flocculating agent. The flocculation rate to kaolin can be up to 95% above. The processing method mainly comprises the steps of adjusting the pH value, heating and centrifuging and so on. The process has the characteristics of low cost, simple process, short preparation time, convenience in application and good processing effect and the like.

The invention relates to an extra-cellular polysaccharide of aerobic Ruthia sp. strain metabolin and preparation and application thereof. An aerobic strain ZHT4-13 is separated from adhesive sludge ofwild Ruditapes philippinarums in the Bohai sea offshore of China and is identified as a Ruthia sp. The aerobic strain is treated by seed culture and amplification culture, and a fermentation broth ofthe aerobic strain is precipitated with ethanol and separated centrifugally to obtain the extra-cellular polysaccharide MBF4-13. The extra-cellular polysaccharide MBF4-13 is measured to have high flocculation activity, the FR value to kaolin reaches over 80 percent, and the removal rate to hexavalent chromium ions reaches 69.3 percent; the extra-cellular polysaccharide MBF4-13 has high activity to decolorize high-concentration wastewater, and the decolorization ratios to methylene blue, ink blue, malachite green and crystal violet reach 86.11 percent, 99.49 percent and 97.84 percent; and theextra-cellular polysaccharide MBF4-13 plays a role of obviously improving the performance and the structure of activated sludge. The extra-cellular polysaccharide has potential value of developing a novel microbial flocculant.

The invention relates to an engineering bacterium with overexpressed uridine diphosphoglucose pyrophosphorylase gene and establishment thereof, belonging to the technical fields of gene engineering and microbial fermentation. By cloning the key enzyme uridine diphosphoglucose pyrophosphorylase gene in the polysaccharide flocculant synthesis route, an Escherichia coli-Bacillus shuttle plasmid is utilized to establish a recombinant expression vector. The recombinant plasmid is transformed into Bacillus licheniformis by electric transformation to establish recombinant Bacillus licheniformis HN301-2; and compared with the initial strain, the polysaccharide flocculant yield of the recombinant Bacillus licheniformis is enhanced by 15.6%, and the fermentation liquid flocculation activity is enhanced by 70%. The engineering bacterium is hopeful to be used in industrial production of polysaccharide flocculants, and can enhance the yield and lower the cost.

The invention discloses a carrier-free immobilized culture method of a microbial flocculating agent. The method is characterized by comprising the following steps of: adding self-flocculating bacteria spore suspension in a volume which is 0.1 to 1 percent of the volume of a culture medium into the culture medium, wherein each millimeter of suspension contains 106 self-flocculating bacteria spores; putting the culture medium into a shaking bed, and performing culture and fermentation, wherein the culture conditions comprise that the revolution speed is 150 to 180 revolutions per minute, the temperature is 20 to 30 DEG C and the pH is 3.5 to 10.5; and standing after the suspension is cultured for 24 to 120 hours, and separating mycelium pellets generated by the self-flocculating bacteria and the fermentation solution, wherein the solid part is the mycelium pellets, and the liquid part is the microbial flocculating agent. Sucrose is used as a carbon source, sodium nitrate is used as a nitrogen source, the self-flocculating bacteria generate stress reaction by using the characteristic that the self-flocculating bacteria can form the mycelium pellets and improving the salt concentration of the culture medium, and the purpose of generating the microbial flocculating agent by the self-flocculating bacteria is fulfilled. The microbial flocculating agent prepared by the method has the advantages of low cost, extensive production conditions and high flocculating activity, and can be used for treating multiple kinds of printing and dyeing wastewater.

The invention relates to a method for preparing a compound flocculating agent, which comprises the following steps of preparing a biological flocculating agent solution; compounding the biological flocculating agent solution and an inorganic flocculating agent solution; dissolving PAC, PFS or the mixture of the PAC and the PFS in deionized water to obtain the inorganic flocculating agent solution, and mixing the biological flocculating agent solution and the inorganic flocculating agent solution according to the volume ratio of 3:10-15; and obtaining the compound flocculating agent. The compound flocculating agent prepared by the method can achieve high flocculating rate and small adding amount and reduce the amount of metal ions remained in the water body, thereby overcoming the defects of the inorganic flocculating agent solution used in water treatment.

The invention discloses a low-production-cost polydopamine compound oil paint flocculant which is prepared by the following steps: (1) separation and purification of strains; (2) screening of the strains; (3) fermentation cultivation of the strains; and (4) preparation of compound magnetic microspheres. The flocculant has the beneficial effects that the flocculation speed is high, the settling time is long, the feeding quantity is small, common components in oil paint can be stably coagulated, the wastewater treatment cost can be lowered, and the application prospects are wide.

The invention discloses a preparation method of a microbial sewage purification treatment agent, and belongs to the technical field of wastewater treatment. According to the invention, a culture medium containing a bacillus natto liquid is prepared from soybean milk, wherein gamma-polyglutamic acid is rich, the gamma-polyglutamic acid can be complexed with metal salt ions in water pollutants to obtain gamma-polyglutamate, the adsorbent has the advantages of biodegradability, good biocompatibility, high water-retaining property and the like, the polypeptide structure of the gamma-polyglutamic acid and carboxyl and amino groups rich in a molecular chain are extremely similar to physiological properties of microorganisms, the gamma-polyglutamic acid is extremely easy to coexist with microorganisms, a space reserved after the gamma-polyglutamic acid is biologically degraded provides a space for subsequent microorganism growth, and the quantity of active microorganisms on the adsorbent is increased, so that the biofilm culturing quantity of the adsorbent microorganisms is increased, and the application prospect is wide.

A preparation method of a biological flocculant, the present invention is characterized in that the fermentation culture of Aspergillus is adopted, and the fermentation culture is stirred for 48 to 60 hours under the conditions of temperature of 28-30° C., pH of 6-7 and ventilation; the fermentation liquid is filtered and concentrated, Ethanol was added to the concentrated solution, and after standing, centrifugation was performed, and the precipitate was washed with ethanol and dried in vacuum to obtain a crude flocculant in light yellow brown. The preparation method of the invention has the advantages of simple production conditions and low cost. The prepared biological flocculant is suitable for sewage treatment containing granular impurities or dirt, and the dosage is generally in the range of 0.1‰ to 0.3‰. It has been proved by the flocculation treatment of sewage that compared with the existing technology, it has the advantages of good flocculation effect, fast flocculation speed, low dosage, no pollution to the environment, and no toxic effect on the human body.

The invention relates to a method for preparing a microbial flocculant which is characterized by: preparing the microbial flocculant through the steps of the preparation of liquid medium, the fermentation culture of the strain, the preparation of the flocculant crude product and flocculant refined product using the delftia acidovorans as the stain resource for preparing the microbial flocculant. The mehtod finds the delftia acidovorans capable of generating the microbial flocculant with a stable flocculability and a good flocculating effect for the first time in China. The screened flocculanthas advantages of high flocculating activity, few dosage, short fermentation time, simple extraction technology, which greatly reduces the production cost. The method for preparing the flocculant is simple and easy to apply. The experiment of wastewater treatment shows that the flocculating effect is ideal after using the flocculant.

The invention discloses a method for preparing a microbial flocculant through enteromorpha hydrolysate fermentation. The method comprises the steps of preparation of liquid strain, fermentation and product aftertreatment. The fermentation process comprises the steps that 1, fresh enteromorpha is cleaned, salt, sediment and unwanted algae are removed, then the fresh enteromorpha is smashed and added to a hydrolysis tank, water is added to regulate acidity, alkali is added for neutralization, filter pressing and clarification are conducted, and then a hydrolysate is obtained; 2, the hydrolysate is diluted till the sugar concentration of reducing sugar is 15-30 g / L, fermentation liquor is prepared and added to a fermentation tank, and high-temperature sterilization and cooling are conducted; 3, a seed solution is inoculated into the fermentation tank for fermentation, and fermentation is ended after the content of residual sugar in the fermentation liquor is reduced to the level below 3 g / L. According to the method, enteromorpha is used as the raw material, microbial flocculant fermentation is directly conducted after acid hydrolysis filtration, the production method is simple, production cost is low, grain is saved, and waste is turned into wealth.

The invention discloses pantoea sp. W36-1, of which the preservation number is CGMCC No. is 3701, a flocculant prepared by the pantoea sp. W36-1 and application of the flocculant in removal of suspensions, dyeing agents and heavy metal in waste water. Extracellular products of the bacteria have an obvious absorption peak at 200 to 250nm, and polysaccharide solution can remove 54.89 percent of free groups in the bacteria. The method for preparing the pantoea sp. W36-1 comprises the following steps of: performing fermentation culture of the bacteria strain for 48 hours, centrifuging 100ml of fermentation solution at a rotation speed of 6,000r / min for 10 minutes, adding absolute ethanol, of which the volume is 1.5 times that of supernatant, into the supernatant, uniformly mixing the absoluteethanol and the supernatant standing the mixed solution at the temperature of 4 DEG C overnight, centrifuging the precipitate, washing the precipitate by 70-percent ethanol for 2 to 3 times, and thendrying the precipitate to obtain the finished product. The flocculent activity of the fermentation culture product of the pantoea sp. W36-1 is 81.7 to 83.15 percent, the yield of the pantoea sp. W36-1 is 6g / L and can be widely used for removing suspensions, dyeing agents, heavy metal and the like in waste water.

The invention relates to a high-temperature-resistant flocculant-producing bacterial strain, and cultivation and application methods thereof, and belongs to the field of microbiology. The high-temperature-resistant flocculant-producing bacterial strain is preserved by China General Microbiological Culture Collection Center (CGMCC), wherein the preservation number is CGMCC No.2040; the classification and nomenclature is Paenibacillus sp.; the preservation date is May 11, 2007. High-temperature-resistant paenibacillus screened from soil of fruit trees is a new bacterium which is discovered in China for the first time; the high-temperature-resistant paenibacillus is heated at a high temperature of 40 to 80 DEG C for 5 to 60 minutes and then is subjected to aerated fermentation culture at 25 to 35 DEG C; when the fermentation liquid is applied to kaolin suspension flocculant, the flocculation ratio is 88% to 97% when the proportion is 0.01% to 0.2% (v / v). When the paenibacillus provided by the invention and the fermentation liquid thereof are applied to the kaolin suspension flocculant, the culture operating process is simple and convenient, the cost is low, the flocculation activity is high, the using amount is small and secondary pollution is avoided.

The invention relates to an engineering bacterium with overexpressed uridine diphosphoglucose pyrophosphorylase gene and establishment thereof, belonging to the technical fields of gene engineering and microbial fermentation. By cloning the key enzyme uridine diphosphoglucose pyrophosphorylase gene in the polysaccharide flocculant synthesis route, an Escherichia coli-Bacillus shuttle plasmid is utilized to establish a recombinant expression vector. The recombinant plasmid is transformed into Bacillus licheniformis by electric transformation to establish recombinant Bacillus licheniformis HN301-2; and compared with the initial strain, the polysaccharide flocculant yield of the recombinant Bacillus licheniformis is enhanced by 15.6%, and the fermentation liquid flocculation activity is enhanced by 70%. The engineering bacterium is hopeful to be used in industrial production of polysaccharide flocculants, and can enhance the yield and lower the cost.